Cyanobacterial akinete induction and its application as biofertilizer for rice cultivation

Abstract

Nostoc sp. VICCR1-1 was induced in order to form akinetes on the basis of nutrient modification. Phosphorus and iron were found to be the critical for akinete differentiation, especially when both elements were omitted. The number of akinete cells increased up to 20% when compared with culturing in BG11 0 medium (without N source). In addition, CaCl 2 played a role in heterocyst differentiation, and was able to induce heterocyst ranging between 30% and 46%. In order to prepare akinetes as inoculum, the dried form of akinetes was prepared by mixing it with montmorillonite clay. The inoculum with the amount of 2.8×10 6 cells m −2 was applied to rice (Oryza sativa) fields. After harvesting, the grain yields from chemical N fertilizer, vegetative cells, and akinete inoculum treatments were not significantly different. To monitor the persistence of Nostoc sp. VICCR1-1 after harvesting, the most probable number-denaturing gradient gel electrophoresis technique using 16S rRNA gene was employed. The results indicated that the remaining population is at 2.5× 10 5 and 1.62×10 6 cells m −2 in treatments supplied with vegetative cells and akinete inocula, respectively. Akinete induction might be one of the appropriate approaches for producing cyanobacterial inoculum.

Potential triggers of akinete differentiation in Nodularia spumigena (Cyanobacteriaceae) isolated from Australia

“…The most commonly investigated cyanobacterium genus is Anabaena (van Dok & Hart, 1996;Li et al, 1997;Sarma et al, 2004;Olli et al, 2005;Thompson et al, 2009), but other genera/species including Nostoc (Innok et al, 2009), Cylindrospermopsis (Moore et al, 2003(Moore et al, , 2005, Scytonema fritschii (Sarma & Ghai, 1998) and Aphanizomenon sp. (Wildman et al, 1975) have also been studied.…”

Review on the biotechnological and nanotechnological potential of the streptophyte genusKlebsormidiumwith pilot data on its phycoprospecting and polyphasic identification in Bulgaria

“…Thus, considering the data on long-term algal survival in soils [126], combined with the biochemical content of Klebsormidium described above and the important role of green algae in soil neogenesis [127,128], we propose the possibility to use inoculums of Klebsormisium akinetes for soil fertilization. This application is similar to the currently actively investigated and applied use of cyanoprokaryote akinetes in the algalization process designed by Venkataraman [129][130][131][132][133][134][135]. The increasing interest in algae as soil biofertilizers and biostimulants in the biotechnological toolbox lies in the benefits which they have shown in the development of green agriculture and nature farming with possibilities for coupling with waste water treatment, bioremediation and energy production [128].…”

Efecto de la Inoculación de Cianobacterias en Cultivos de Interés Comercial en Zonas Semiáridas de La Guajira - Colombia

“…Los resultados revelan un incremento en rendimiento de grano entre 15 y 20% en experimentos de campo (Innok et al, 2009). La inoculación no solo mejora el rendimiento de grano sino el aumento en el contenido de N del grano, el crecimiento vegetal (altura de planta, longitud de la hoja bandera biomasa vegetal y el número de macollas) y los componentes de rendimiento (Vaishampayan et al, 2001).…”