MnO₂-Nanosheet-Modified Upconversion Nanosystem for Sensitive Turn-On Fluorescence Detection of H₂O₂and Glucose in Blood

Abstract

Blood glucose monitoring has attracted extensive attention because diabetes mellitus is a worldwide public health problem. Here, we reported an upconversion fluorescence detection method based on manganese dioxide (MnO2)-nanosheet-modified upconversion nanoparticles (UCNPs) for rapid, sensitive detection of glucose levels in human serum and whole blood. In this strategy, MnO2 nanosheets on the UCNP surface serve as a quencher. UCNP fluorescence can make a recovery by the addition of H2O2, which can reduce MnO2 to Mn(2+), and the glucose can thus be monitored based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2. Because of the nonautofluorescent assays offered by UCNPs, the developed method has been applied to monitor glucose levels in human serum and whole blood samples with satisfactory results. The proposed approach holds great potential for diabetes mellitus research and clinical diagnosis. Meanwhile, this nanosystem is also generalizable and can be easily expanded to the detection of various H2O2-involved analytes.

One step electrosynthesis of conjugated polymers thin film for Fe3+ detection and its potential application

“…The recoveries of the samples were found to be in the range of 93.7-105.6% with the RSD within 5.0%. The recoveries of the samples and the RSD values were satisfactory compared with some reports [57][58][59][60][61], which proved the reliability of the proposed sensing platform for Glu determination in real samples.…”

Fabrication of a LRET-based upconverting hybrid nanocomposite for turn-on sensing of H2O2and glucose

“…[43][44][45][46][47][48][49] In most cases, the UCNPs are decorated with a target recognizing unit (the energy acceptor) to form the luminescence resonance energy transfer (LRET)based hybrid nanosystems. 54,55 Wang' group proposed a glucose biosensor based on the luminescence quenching of UCNPs by oxidization products of 3,3',5,5'tetramethylbenzidine (TMB). 54,55 Wang' group proposed a glucose biosensor based on the luminescence quenching of UCNPs by oxidization products of 3,3',5,5'tetramethylbenzidine (TMB).…”

Self-assembly synthesis of Co3O4/multiwalled carbon nanotube composites: an efficient enzyme-free glucose sensor

“…1-3 Among carbon materials, CNTs are considered to be a potentially promising candidate because of their low cost, good conductivity, easily functionalized properties, large surface areas and excellent corrosion resistance in various electrolytes. [9][10][11][12][13][14][15][16] Generally these metal oxide based sensors directly electrooxidise the glucose mediated by M 2+ /M 3+ (M = metal) in alkaline medium. [6][7][8] Many researchers around the world have focused on the development of CNT decoration with metal and metal-oxide nanoparticles (NPs) to obtain the active components for non-enzymatic glucose sensors such as NiO, CuO, Co 3 O 4 , MnO 2 , etc.…”