Mo1560 Impaired Autophagy in Gastric Carcinoids and Adenocarcinoma of Both Rodent Models and Patients

Vigen, Reidar Alexander; Kodama, Yosuke; Viset, Trond; Fossmark, Reidar; Waldum, Helge L.; Kidd, Mark; Wang, Yichen; Modlin, Irvin; Chen, Duan; Zhao, Chun‐Mei

doi:10.1016/s0016-5085(12)62415-9

Cited by 8 publications

(12 citation statements)

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“…Beclin-1, the first identified mammalian autophagy effector, was reported to be deleted or significantly decreased in ovarian, breast and prostate cancer, suggesting that autophagy may be a tumor-suppressive mechanism (12). Notably, previous studies have indicated that low expression levels of Beclin-1 were predictive of poor survival in B cell lymphoma-xL-overexpressing hepatocellular carcinoma tissues (2,3). These observations indicate that autophagy has a significant role in tumorigenesis and prognosis.…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

“…Although favorable therapeutic outcomes for the treatment of gastric cancer have been achieved by a combination of surgery and chemotherapy, distant metastasis and recurrence remain common in advanced stage patients (2,3). Progression of the tumor cells may be induced by a variety of factors, including multiple structural and functional genetic alterations, cell-cycle regulators, adhesion molecules and growth factors (2). Study of these factors is of significant clinical value for further understanding the underlying molecular mechanisms of gastric cancer progression, as well as identifying novel therapeutic targets in gastric tissues (4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

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Expression and clinical significance of Beclin-1 in gastric cancer tissues of various clinical stages

Fei

Chen

et al. 2016

Oncology Letters

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression and clinical significance of Beclin-1 in gastric cancer tissues of various clinical stages

Fei

Chen

et al. 2016

Oncology Letters

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“…The volumes of GC xenografts were assessed every 5 days. After 30 days of implantation, the mice were sacri ced and the tumor volume in mm 3 was calculated by the formula1/2 (length× width 2 ). For the peritoneal dissemination assay, 1×10 6 TRPV1-overexpression BGC823 cells and the NC cells were injected into the abdominal cavity of nude mice.…”

Section: Tumor Xenograft and Peritoneal Dissemination Assay In Nude Micementioning

confidence: 99%

“…Gastric cancer (GC) is the second most common human cancers worldwide and is di cult to be diagnosed in its early stage [1]. GC is extremely hard to be cured once it develops into metastasis stage [2,3]. Although the occurrence and progression of cancer are complex, numerous evidences indicate that aberrant intracellular Ca 2+ ([Ca 2+ ] i ) signaling is involved in the development of several types of gastrointestinal (GI) cancers, including GC and colon cancer [4].…”

Section: Introductionmentioning

confidence: 99%

A Unique Role Of TRPV1 Ion Channels In The Suppression Of Gastric Cancer Development

Gao

Feng

Chen

et al. 2020

Preprint

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Background: Although the aberrant expression and function of most Ca2+-permeable channels are known to promote gastrointestinal tumors, the association of transient receptor potential vanilloid receptor 1(TRPV1) channels and gastric cancer (GC) has not been explored so far. We sought to determine their role in the development of GC and to elucidate the underlying molecular mechanisms. Methods: The expression of TRPV1 in GC cells and tissues was detected by qPCR, immunohistochemistry, western blot analysis and immunofluorescence. CCK8 and flow cytometry were used to detect the proliferation and cell cycle, while transwell assay was used to detect migration and invasion. The role of TRPV1 in GC development in vivo was tested using tumor xenograft and peritoneal dissemination assays in nude mice. Results: The decreased expression of TRPV1 protein in primary human GC tissues was closely correlated with poor prognosis of GC patients. TRPV1 protein was predominately expressed on the plasma membrane of several GC cell lines. TRPV1 overexpression attenuated proliferation, migration and invasion of GC cells in vitro, but TRPV1 knockdown increased them. Moreover, TRPV1 significantly reduced gastric tumor sizes, numbers and peritoneal dissemination in vivo. Mechanistically, TRPV1 overexpression increased [Ca2+]i, activated CaMKKβ and AMPK phosphorylation, and decreased expression of cyclin D1 and MMP2, but TRPV1 knockdown caused the opposite effects.Conclusions: TRPV1 uniquely suppresses GC through a novel Ca2+/CaMKKβ/AMPK pathway and its downregulation is correlated with poor survival in human GC. TRPV1 upregulation and its downstream signaling may be a promising strategy for GC prevention and therapy.

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Hematologic and serum biochemistry reference intervals using defined ASCVP methodology for laboratory natal multimammate mice (Mastomys natalensis)

et al. 2021

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Complete blood count, serum chemistry values, and biological reference intervals were compared between two age groups (34–49 and 84–120 days old) of healthy male and female laboratory raised natal multimammate mice ( Mastomys natalensis). Blood was collected via cardiocentesis under isoflurane anesthesia. Data sets of machine automated complete blood counts and clinical chemistries were analyzed. Significant differences between sex and age groups of the data sets were defined. The baseline hematologic and serum biochemistry values described here can improve interpretation of laboratory research using natal multimammate mice.

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Mo1560 Impaired Autophagy in Gastric Carcinoids and Adenocarcinoma of Both Rodent Models and Patients

Cited by 8 publications

References 0 publications

Expression and clinical significance of Beclin-1 in gastric cancer tissues of various clinical stages

Expression and clinical significance of Beclin-1 in gastric cancer tissues of various clinical stages

A Unique Role Of TRPV1 Ion Channels In The Suppression Of Gastric Cancer Development

Hematologic and serum biochemistry reference intervals using defined ASCVP methodology for laboratory natal multimammate mice (Mastomys natalensis)

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