Mobilization and synthesis of seed storage and LEA proteins during natural priming of Buddleja cordata and Opuntia tomentosa

Abstract

Background: In crop plants, the induction of seed storage protein mobilization and synthesis of LEA family proteins has been extensively described.Question: Natural priming promotes mobilization and synthesis of seed storage and LEA proteins in wild plants?Species studied: Buddleja cordata and Opuntia tomentosa. Study site: Reserva Ecológica del Pedregal de San Angel (REPSA). Methods: Natural priming treatments were applied to B. cordata and O. tomentosa seeds during one and six months respectively. Phosphorylated proteins fractions were obtained by affinity chromatography from control and treated seeds. Differences in protein pattern between control and treated seeds were determined by electrophoresis and the treated seed proteins were identified by LS/LS/MALDITOF. Results:The phosphorylated fraction of B. cordata treated seeds presented two proteins that were absent in the control protein fraction. These two proteins were identified as two different 11S globulins. The phosphorylated fraction of O. tomentosa treated seeds also presented two proteins that were absent in the control protein fraction. These two proteins were identified as a 12S globulin and a LEA protein. Conclusion:Seeds are subjected to changes in soil water content during their permanence in soil; this natural priming process promotes mobilization and synthesis of storage and LEA proteins that are involved in stress resistance.Key words: Buddleja cordata, natural priming, Opuntia tomentosa, seed proteins. ResumenAntecedentes: La inducción de la movilización y síntesis de proteínas de reserva y de proteínas de la familia LEA en respuesta al acondicionamiento es un proceso ampliamente descrito en plantas cultivadas.Preguntas: ¿El acondicionamiento natural promueve la movilización y síntesis de las proteínas de reserva y LEA en plantas silvestres?Especies: Buddleja cordata y Opuntia tomentosa. Métodos: Se aplicó el tratamiento de acondicionamiento natural a las semillas de B. cordata y O. tomentosa durante 1 y 6 meses respectivamente. La fracción de proteínas fosforiladas se obtuvo por medio de una cromatografía de afinidad de las semillas control y tratadas. Las diferencias en el patrón proteico entre semillas control y tratadas se determinaron por medio de una electroforesis y las proteínas de las semillas tratadas fueron identificadas por LS/LS/MALDITOF. Resultados:En las semillas tratadas de B. cordata se detectaron dos proteínas identificadas como 11S globulinas y en las semillas tratadas de O. tomentosa se detectaron dos proteínas correspondientes a una 12S globulina y una proteína LEA. Conclusiones:Durante su estancia en el suelo, las semillas están sujetas a cambios en el contenido de agua del suelo y este proceso de acondicionamiento natural promueve la movilización y síntesis de proteínas de reserva y LEA en las semillas, involucradas en la resistencia a estrés.Palabras clave: Acondicionamiento natural, Buddleja cordata, Opuntia tomentosa, proteínas de la semilla. Botanical Sciences (1): 76-83, 2018This is an open access article ...

Effects of seed priming on germination and seedling growth of desiccation-sensitive seeds from Mexican tropical rainforest

“…During this time, seeds undergo physiological and biochemical germination-related changes (Alvarado-López et al 2018;González-Zertuche et al 2001;Long et al 2011). Additionally, stress resistance-related compounds have been found in the natural primed seeds of native species (Alvarado-López et al 2018;González-Zertuche et al 2001). We did not evaluate the stress tolerance of primed seeds; hence, further studies should analyze this capacity.…”

“…Recalcitrant seeds maintain an active metabolism after shedding (Berjak and Pammenter 2013), which might be driven by favorable moisture and temperature conditions in the soil. During this time, seeds undergo physiological and biochemical germination-related changes (Alvarado-López et al 2018;González-Zertuche et al 2001;Long et al 2011). Additionally, stress resistance-related compounds have been found in the natural primed seeds of native species (Alvarado-López et al 2018;González-Zertuche et al 2001).…”

“…Natural priming is a matrix priming variant that consists of burying in situ desiccation-tolerant seeds within a period ranging from seed dispersal to the beginning of the rainy season (González-Zertuche et al 2001;Orozco-Segovia et al 2014). After burial, unearthing, and dehydration, the seeds acquire the germination-related processes to invigorate them (Alvarado-López et al 2018;González-Zertuche et al 2001), which can even improve the growth and survival of seedlings (Benítez-Rodríguez et al 2014;Peraza-Villarreal et al 2018). In the seed bank, seeds undergo hydration-dehydration cycles in accordance with the moisture variation in the soil (Hadas 2004;Long et al 2015).…”

Changes in Growth and Heavy Metal and Phenolic Compound Accumulation in Buddleja cordata Cell Suspension Culture under Cu, Fe, Mn, and Zn Enrichment

“…Bonpl. and Kunth is distributed in a wide range of habitats in Mexico, including habitats with infertile soils [ 13 , 14 ], the most recent study to determine its hyperaccumulation potential was published by Navarrete et al [ 14 ]. In that work, the ultramafic vegetation of central and southern Mexico was assessed to identify Ni hyperaccumulator species.…”