Mobilization of Tn1721-like structure harboring blaCTX-M-27 between P1-like bacteriophage in Salmonella and plasmids in Escherichia coli in China

Zhao, Qiu-Yun; Li, Wan; Cai, Run-Mao; Lu, Yaqin; Zhang, Yan; Cai, Peng; Webber, Mark A.; Jiang, Hongxia

doi:10.1016/j.vetmic.2020.108944

Cited by 12 publications

(18 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We surveyed two swine farms in Guangdong province and confirmed the presence of bla CTX-M-27 in 32 E. coli isolates including 62.5% (20) from farm 1 and 37.5% (12) from farm 2 (Figure 1). CTX-M-27 has been only sporadically detected in E. coli isolates from food-producing animals, and its prevalence has remained constant at <8% over the past decade [17,19]. The prevalence of bla CTX-M-27 in our study (9.61%) was higher than in previous studies.…”

Section: Phenotypes Of Bla Ctx-m-27 -Carrying Isolatescontrasting

confidence: 74%

“…These data indicated that the transmission of bla CTX-M-27 among E. coli isolates occurred via clonal expansion and horizontal transmission. The results of both clonal spread of resistant strains and horizontal transmission of the resistance plasmids contributed to the dissemination of bla CTX-M-27 -positive salmonella or E. coli isolates were also obtained in previous studies [16,17,39].…”

Section: Molecular Typing Analysissupporting

confidence: 58%

“…Recent reports documented that ST10 and ST224 E. coli are primarily carriers of CTX-M-15, CTX-M-14, CTX-M-1 and CTX-M-8 [31][32][33][34][35][36][37]. Our recent study identified ST8900 as the most prevalent ST (Sequence Typing) in CTX-M-27-positive E. coli isolates obtained during 2003 to 2009 [17]. These results indicate that the dominant ST of blaCTX-M-27-producing E. coli has changed.…”

Section: Molecular Typing Analysissupporting

confidence: 51%

“…Importantly, all 32 CTX-M-27 isolates were multidrug-resistant (MDR) strains (Table S1). The prevalence of ciprofloxacin-resistance in these isolates was 93.75% compared with 77.78% from isolates in 2003 to 2009 in China on swine farms [17], and the prevalence of ciprofloxacin resistance in bla CTX-M-14 or bla CTX-M-15 -producing E. coli almost above 80% [20,21]. Additionally, we detected QRDR (Quinolone Resistance Determining Regions) mutations in gyrA (S83L and D87N) and parC (S80I) in high-level quinolone resistant isolates (28), and 14 of these coharbored oqxAB (Figure 1).…”

Section: Phenotypes Of Bla Ctx-m-27 -Carrying Isolatesmentioning

confidence: 81%

“…The prevalence of bla CTX-M-27 in Salmonella isolated from food animals is also increasing, and transduction of bla CTX-M-27 in Salmonella isolated from pork has been recently demonstrated to be mediated by a P1-like bacteriophage that had integrated a Tn1721-like structure [16]. A prospective study utilizing salmonella and E. coli isolates from 2003 to 2014 indicated that bla CTX-M-27 could be mobilized via a Tn1721-like structure between E. coli plasmids and P1-like bacteriophage in salmonella [17].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A Novel Structure Harboring blaCTX-M-27 on IncF Plasmids in Escherichia coli Isolated from Swine in China

et al. 2021

Self Cite

View full text Add to dashboard Cite

The aim of this study was to elucidate the prevalence of blaCTX-M-27-producing Escherichia coli and transmission mechanisms of blaCTX-M-27 from swine farms in China. A total of 333 E. coli isolates were collected from two farms from 2013 to 2016. Thirty-two CTX-M-27-positive E. coli were obtained, and all were multidrug-resistant. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) profiles indicated a wide range of strain types that carried blaCTX-M-27, and the sequence type ST10 predominated. Conjugation, replicon typing, S1-PFGE and hybridization experiments confirmed that 28 out of 32 CTX-M-27 positive isolates carried blaCTX-M-27 genes on plasmids F18:A-:B10 (16) and F24:A-:B1 (12).The blaCTX-M-27 genes for 24 isolates were transmitted by plasmids with sizes ranging from 40 to 155 kb. A comparative analysis with blaCTX-M-27-plasmids indicated that the tra-trb region of F24:A-:B1 plasmids was destroyed by insertion of a complex region (eight isolates) and a novel structure containing blaCTX-M-27 in the F18:A-:B10 plasmids (12 isolates). The novel structure increased the stability of the blaCTX-M-27 gene in E. coli. This study indicated that the predominant vehicle for blaCTX-M-27 transmission has diversified over time and that control strategies to limit blaCTX-M-27 transmission in farm animals are necessary.

show abstract

Section: Phenotypes Of Bla Ctx-m-27 -Carrying Isolatescontrasting

confidence: 74%

Section: Molecular Typing Analysissupporting

confidence: 58%