Model‐based analysis of a dielectrophoretic microfluidic device for field‐flow fractionation

Mathew, Bobby; Alazzam, Anas; Abutayeh, Mohammad; Stiharu, Ion

doi:10.1002/jssc.201600350

Cited by 22 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Combining Eqs. (11) and (12), we are able to describe the iterative method for particle tracing as follows [40]: r n+1 = 2r n r n 1 + 0:5 F n m dt 2 :…”

Section: Particle Tracing Methodsmentioning

confidence: 99%

Numerical study of insulation structure characteristics and arrangement effects on cell trapping using alternative current insulating based dielectrophoresis

2019

View full text Add to dashboard Cite

Insulator-based dielectrophoresis is a recently developed technique in which insulating posts are used to produce non-uniformity in the electric eld in a microchannel. This study presents the e ects of insulating posts' geometry and arrangement on the trapping e ciency of red blood cells in an alternating current-insulator-based dielectrophoresis system. Microchannels containing square, circular, and diamond-shaped posts with particles under the in uence of positive dielectrophoresis force and uid ow were considered in this study. The nite element method was used to compute the velocity of the ow and electric eld. The numerical method was veri ed by comparing the numerical results with experimental data. Two distinct criteria for examining particle trapping for distinct shapes and arrangements of insulating posts were introduced. Particle tracing simulation was implemented to observe particle trapping and compare the trapping performance of systems with distinct posts. As shown in the results for the system with circular and square posts, insulators should be narrowed to improve particle trapping, while the diamond post should be widened to increase the trapping e ciency. In addition, the particle tracking results showed that the microchannel with square posts was more e cient in particle trapping.

show abstract

“…Combining Eqs. (11) and (12), we are able to describe the iterative method for particle tracing as follows [40]: r n+1 = 2r n r n 1 + 0:5 F n m dt 2 :…”

Section: Particle Tracing Methodsmentioning

confidence: 99%

Numerical study of insulation structure characteristics and arrangement effects on cell trapping using alternative current insulating based dielectrophoresis

2019

View full text Add to dashboard Cite

show abstract

“…Cells will levitate to heights, in a DEP‐FFF separation microdevice, depending on their properties and in the process achieve separation. The height to which a cell levitates depends on properties such as conductivity, permittivity, and density of the medium and the cell .…”

Section: Introductionmentioning

confidence: 99%

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

2017

Self Cite

View full text Add to dashboard Cite

We describe the design, microfabrication, and testing of a microfluidic device for the separation of cancer cells based on dielectrophoresis. Cancer cells, specifically green fluorescent protein-labeled MDA-MB-231, are successfully separated from a heterogeneous mixture of the same and normal blood cells. MDA-MB-231 cancer cells are separated with an accuracy that enables precise detection and counting of circulating tumor cells present among normal blood cells. The separation is performed using a set of planar interdigitated transducer electrodes that are deposited on the surface of a glass wafer and slightly protrude into the separation microchannel at one side. The device includes two parts, namely, a glass wafer and polydimethylsiloxane element. The device is fabricated using standard microfabrication techniques. All experiments are conducted with low conductivity sucrose-dextrose isotonic medium. The variation in response between MDA-MB-231 cancer cells and normal cells to a certain band of alternating-current frequencies is used for continuous separation of cells. The fabrication of the microfluidic device, preparation of cells and medium, and flow conditions are detailed. The proposed microdevice can be used to detect and separate malignant cells from heterogeneous mixture of cells for the purpose of early screening for cancer.

show abstract

“…Regarding the numerical simulation of DEP microfluidic devices, Matthew et al. conducted a study to investigate the effect of various parameters on the performance of a numerically simulated DEP device. It was concluded that the microchannel depth, electrode spacing lengths, and voltage affect the levitation height of the microparticles and the flow rate and microparticle radius were not influential.…”

Section: Introductionmentioning

confidence: 99%

“…Among the applications that computational simulations of microfluidic devices can help are blood-related separation purposes [15,16], cell separation procedures [17,18], and mixing [19,20]. Regarding the numerical simulation of DEP microfluidic devices, Matthew et al [21] conducted a study to investigate the effect of various parameters on the performance of a numerically simulated DEP device. It was concluded that the microchannel depth, electrode spacing lengths, and voltage affect the levitation height of the microparticles and the flow rate and microparticle radius were not influential.…”

Section: Introductionmentioning

confidence: 99%

Numerical analysis of a dielectrophoresis field‐flow fractionation device for the separation of multiple cell types

Shamloo

Kamali

2017

J of Separation Science

View full text Add to dashboard Cite

In this study, a dielectrophoresis field-flow fractionation device was analyzed using a numerical simulation method and the behaviors of a set of different cells were investigated. By reducing the alternating current frequency of the electrodes from the value used in the original setup configuration and increasing the number of exit channels, total discrimination in cell trajectories and subsequent separation of four cell types were achieved. Cells were differentiated based on their size and dielectric response that are represented in their real part of Clausius-Mossotti factor at different frequencies. A number of novel designs were also proposed based on the original setup configuration. It was seen that by reducing the length of the main channel and the number of electrodes at low frequencies and not changing the inlet flow velocities, cell separation was still achieved successfully, although with a slightly larger electrode voltage. The shorter main channel decreased the residence time for the cells on the chip and also reduced the overall size of the device-these were improvements over the original design. The obtained results can be used to analyze other cell types by knowing their size and dielectric properties to design geometries that can ensure separation.

show abstract

Model‐based analysis of a dielectrophoretic microfluidic device for field‐flow fractionation

Cited by 22 publications

References 31 publications

Numerical study of insulation structure characteristics and arrangement effects on cell trapping using alternative current insulating based dielectrophoresis

Numerical study of insulation structure characteristics and arrangement effects on cell trapping using alternative current insulating based dielectrophoresis

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

Numerical analysis of a dielectrophoresis field‐flow fractionation device for the separation of multiple cell types

Contact Info

Product

Resources

About