Silky Oak or Silver Oak (Grevillea robusta A.Cunn. ex R.Br.) is classified as a high-value economic wood in Thailand, it is also considered to be a plant that can grow rapidly, and it has the potential to efficiently reduce greenhouse gases emitted into the atmosphere. This research aimed to study and develop an allometric equation to evaluate the biomass of F1 Silky Oak, which was imported to Thailand from Australia, and grown in Thailand’s economic woods in Silky Oak sites in Pak Chong District, Nakhon Ratchasima Province. The sample group consisted of trees of different ages (i.e., of 2 years, 3–4 years, and 7 years). An allometric equation was used to determine the tree biomass, based on mathematical models that describe the relationship between tree biomass and diameter at breast height (DBH). It was developed in the form of a quadratic equation by multiplying the square DBH by the total height (DBH2 × Ht). Subsequently, the equation was separated into different components, which corresponded with different parts of the tree (i.e., stem, branches, leaves, and roots). The following equations were obtained for the stem: Ws = 0.0721 (D2H) 0.8297 R2 = 0.998. The following equations were obtained for the branches: Wb = 0.0772 (D2H) 0.7027 R2 = 0.977. The following equations were obtained for the leaves, Wl = 0.2085 (D2H) 0.4313 R2 = 0.990. The following equations were obtained for the roots: Wr = 0.3337 (D2H) 0.4886 R2 = 0.957. The results of a laboratory elemental analysis of the carbon sequestration in the biomass, using a CHN elemental analyzer, showed that the mean percentage of carbon content in the stems, branches, leaves, and roots was 45.805. Applying the developed allometric equation for evaluating carbon sequestration, using the survey data from the sample sites of Silky Oak, it was found that the amount of carbon sequestration for the aboveground biomass in three sites was 130.63 tCO2eq. When the amount was converted into carbon dioxide, which was absorbed in the three sites, we obtained a value of 478.99 tCO2eq. The results of the application of the allometric equation showed that there was substantial carbon sequestration potential in the surveyed sites, emphasizing the role of Silky Oak plantations for climate change mitigation and sustainable land management. This study advances our understanding of Silky Oak growth and carbon storage dynamics, offering valuable tools for biomass estimation and promoting environmentally beneficial land use practices.