Model of sepsis (Caecal Ligation and Puncture) in rats caused by mixed and pure bacterial cultures and changes in white blood cell counts

Dragica, Stojanovic; Ašanin, Ružica; Malicevic, Zivorad; Branka, M Vidic

doi:10.2298/avb0404281s

Acta vet. (Beogr.)

2004

DOI: 10.2298/avb0404281s

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Model of sepsis (Caecal Ligation and Puncture) in rats caused by mixed and pure bacterial cultures and changes in white blood cell counts

Stojanovic Dragica¹,

Ružica Ašanin²,

Zivorad Malicevic³

et al.

Abstract: The number of leucocytes and immunocompetent cells, was investigated during a clinical form of sepsis in rats. The experiments were carried out on 104 male rats, Wistar strain, of body weight 190 to 240 g. The rats were divided into four groups: three with 28 animals and one control group with 20 animals. The animals were killed 12, 24, 72 or 120 hours after surgical intervention. This consisted of caecal ligation and puncture (CLP), with inoculation of mixed bacteria or pure cultures of Escherichia coli or St… Show more

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Cited by 2 publications

References 12 publications

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Brain stem and thalamus antioxidative defense in experimental sepsis

Ninković¹,

Malicevic²,

Dragica³

et al. 2008

Acta vet. (Beogr.)

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Although brain complications in sepsis are not rare, early pathophysiologic events had not been made clear yet. We have considered antioxidative components-glutathione peroxidase (GSHPx) activity and reduced glutathione (GSH) concentration in two brain integrative centers, i.e the brain stem (BS) and thalamus. Sepsis was induced in adult male Wistar rats (200-250 g) by cecal ligation and perforation (CLP) with inoculation of Escherichia coli suspension (ATCC 25922) (n=40). The control group was sham operated (n=40). For each time point (0, 12, 24 and 72 hours) after treatment, ten animals within each group were decapitated. In BS, GSHPx activity increased at 12 and 24 hours after CLP, while in the thalamus, GSHPx activity increased at 72 hours, compared to controls. In BS, GSH concentration decreased at the 12th and 24th hour, and in the thalamus it decreased at the 72nd hour. Changed oxidative status in BS, recorded as soon as the 12th hour, reflects a prompt reaction of the central nervous system. This could be of great consequence for disturbed vasomotor response during sepsis.

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Brain stem and thalamus antioxidative defense in experimental sepsis

Ninković¹,

Malicevic²,

Dragica³

et al. 2008

Acta vet. (Beogr.)

Self Cite

View full text Add to dashboard Cite

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Examination of enzymes concentration in the blood of rats with sepsis caused by mixed and pure bacterial cultures

Dragica¹,

Kovačević²,

Zekic³

et al. 2013

ACTA VET-BEOGRAD

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A clinical form of sepsis induced by cecal ligation and puncture (CLP) was caused in order to monitor the concentration of enzymes (alanine aminotransferase -ALT, aspartate aminotransferase -AST, lactate dehydrogenase -LDH, amylase and creatine kinase -CK) in the rat blood. Experiments were performed on male Wistar rats, weighing on average 215±25 g. The rats were divided into four groups. In the first three groups (n=28 per group), sepsis was induced by pure culture of Escherichia coli (EC) or Staphylococcus aureus (SA) and mixed culture (MK) of caecum, while the fourth group included 20 control rats who underwent an abdominal incision. Blood was taken in time intervals of 12, 24, 72 and 120 hours.During the experimental protocol, we identified significant changes of all monitored enzymes in the serum of infected rats. After a period of 12 hours there was a significant increase in ALT (all rats with sepsis), AST and LDH (rats in the MK group) levels, while a decrease was noted in the concentration of amylase (EC, SA). Similarly, 24 hours after the CLP procedure, a significant decrease of amylase (MK) and AST (SA) was recorded, while serum LDH level varied significantly from elevated (EC, SA) to reduced (MC) values. Finally, at the time intervals of 72 and 120 hours the concentration of nearly all monitored enzymes has shown a decline, while significance was noted in lowering of ALT (MK), AST (SA, EC) and amylase (SA) levels. Statistical significance could not be observed in the change of CK levels at any of examined time points.

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Model of sepsis (Caecal Ligation and Puncture) in rats caused by mixed and pure bacterial cultures and changes in white blood cell counts

Cited by 2 publications

References 12 publications

Brain stem and thalamus antioxidative defense in experimental sepsis

Brain stem and thalamus antioxidative defense in experimental sepsis

Examination of enzymes concentration in the blood of rats with sepsis caused by mixed and pure bacterial cultures

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