The thorough investigation of both grain threshing and grain separating processes is a crucial consideration for effective structural design and variable optimization of the tangential flow threshing cylinder and longitudinal axial flow threshing cylinder composite units (TLFC unit) of small and medium-sized (SME) combine harvesters. The objective of this paper was to obtain the structural variables of a TLFC unit by theoretical modeling and experimentation on a tangential flow threshing cylinder unit (TFC unit) and longitudinal axial flow threshing cylinder unit (LFC unit). Threshing and separation equations for five types of threshing teeth (knife bar, trapezoidal tooth, spike tooth, rasp bar, and rectangular bar), were obtained using probability theory. Results demonstrate that the threshing and separation capacity of the knife bar TFC unit was stronger than the other threshing teeth. The length of the LFC unit was divided into four sections, with helical blades on the first section (0-0.17 m), the spike tooth on the second section (0.17-1.48 m), the trapezoidal tooth on the third section (1.48-2.91 m), and the discharge plate on the fourth section (2.91-3.35 m). Test results showed an un-threshed grain rate of 0.243%, un-separated grain rate of 0.346%, and broken grain rate of 0.184%. Evidenced by these results, threshing and separation performance is significantly improved by analyzing and optimizing the structure and variables of a TLFC unit. The results of this research can be used to successfully design the TLFC unit of small and medium-sized (SME) combine harvesters.