Modeling cell entry into a micro-channel

Leong, Fong Yew; Li, Qingsen; Lim, Chwee Teck; Chiam, Keng-Hwee

doi:10.1007/s10237-010-0271-1

Cited by 66 publications

(54 citation statements)

References 43 publications

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“…As the front of the cell gradually leaves the micro-channel, it restores its original structure with increasing velocity. Those results are agrees with experimental observations [138] and the results obtained from compound drop model [153]. The obtained patterns of cell deformation, contraction and expansion as well the recovery of its original shape are similar to experimental observations.…”

Section: Movement and Deformation Of Single Cellssupporting

confidence: 82%

See 1 more Smart Citation

Dissipative Particle Dynamics (DPD): An Overview and Recent Developments

Liu

Zhou

et al. 2014

Arch Computat Methods Eng

178

101

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Dissipative particle dynamics (DPD) is a mesoscale particle method that bridges the gap between microscopic and macroscopic simulations. It can be regarded as a coarse-grained molecular dynamics method suitable for larger time and length scales. It has been successfully applied to different areas of interests, especially in modeling the hydrodynamic behavior of complex fluids in mesoscale. This paper presents an overview on DPD including the methodology, formulation, implementation procedure and some related numerical aspects. The paper also reviews the major applications of the DPD method, especially in modeling (1) micro drop dynamics, (2) multiphase flows in microchannels and fracture networks, (3) movement and suspension of macromolecules in micro channels and (4) movement and deformation of single cells. The paper ends with some concluding remarks summarizing the major features and future possible development of this unique mesoscale modeling technique.

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Section: Movement and Deformation Of Single Cellssupporting

confidence: 82%

“…Recently, Leong et al presented a modified compound drop model, which can consider stiffness, elasticity, and viscosity of both the cortex and the nucleus to model breast cancer cell entry into a constricted micro-channel. The modeled cell entry behavior agrees with experimental observations [153].…”

Section: Movement and Deformation Of Single Cellssupporting

confidence: 79%

Dissipative Particle Dynamics (DPD): An Overview and Recent Developments

Liu

Zhou

et al. 2014

Arch Computat Methods Eng

178

101

View full text Add to dashboard Cite

show abstract

“…Experimental work on red blood cells [3] and polymeric capsules [7] has shown that changes in mechanical properties and cell radius determine the passage of the container and in some cases may induce capillary blockage [8,9]. Previous theoretical work has extensively studied the transient dynamics of elastic capsules in both cylindrical and rectangular constrictions [10][11][12], the production of smaller vesicles [13], and the translocation of vesicles through narrow pores [14].…”

Section: Introductionmentioning

confidence: 99%

Forced transport of deformable containers through narrow constrictions

et al. 2014

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We study, numerically and analytically, the forced transport of deformable containers through a narrow constriction. Our central aim is to quantify the competition between the constriction geometry and the active forcing, regulating whether and at which speed a container may pass through the constriction and under what conditions it gets stuck. We focus, in particular, on the interrelation between the force that propels the container and the radius of the channel, as these are the external variables that may be directly controlled in both artificial and physiological settings. We present Lattice-Boltzmann simulations that elucidate in detail the various phases of translocation, and present simplified analytical models that treat two limiting types of these membrane containers: deformational energy dominated by the bending or stretching contribution. In either case we find excellent agreement with the full simulations, and our results reveal that not only the radius but also the length of the constriction determines whether or not the container will pass.Comment: 9 pages, 4 figure

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“…Indeed, flat channel walls are well adapted to observe cell-surface interactions by optical microscopy during cell transit in a capillary. Efforts in modeling have been undertaken for the entry of a cell in a simple constriction, 45,46 but they omit leakage and friction effects. A microfluidic micropipette method was recently described to measure the cell cortical tension, but not the viscous properties, or loss modulus, of cells.…”

Section: Introductionmentioning

confidence: 99%

Single cell rheometry with a microfluidic constriction: Quantitative control of friction and fluid leaks between cell and channel walls

et al. 2013

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We report how cell rheology measurements can be performed by monitoring the deformation of a cell in a microfluidic constriction, provided that friction and fluid leaks effects between the cell and the walls of the microchannels are correctly taken into account. Indeed, the mismatch between the rounded shapes of cells and the angular cross-section of standard microfluidic channels hampers efficient obstruction of the channel by an incoming cell. Moreover, friction forces between a cell and channels walls have never been characterized. Both effects impede a quantitative determination of forces experienced by cells in a constriction. Our study is based on a new microfluidic device composed of two successive constrictions, combined with optical interference microscopy measurements to characterize the contact zone between the cell and the walls of the channel. A cell squeezed in a first constriction obstructs most of the channel cross-section, which strongly limits leaks around cells. The rheological properties of the cell are subsequently probed during its entry in a second narrower constriction. The pressure force is determined from the pressure drop across the device, the cell velocity, and the width of the gutters formed between the cell and the corners of the channel. The additional friction force, which has never been analyzed for moving and constrained cells before, is found to involve both hydrodynamic lubrication and surface forces. This friction results in the existence of a threshold for moving the cells and leads to a non-linear behavior at low velocity. The friction force can nevertheless be assessed in the linear regime. Finally, an apparent viscosity of single cells can be estimated from a numerical prediction of the viscous dissipation induced by a small step in the channel. A preliminary application of our method yields an apparent loss modulus on the order of 100 Pa s for leukocytes THP-1 cells, in agreement with the literature data. V C 2013 AIP Publishing LLC [http://dx

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Modeling cell entry into a micro-channel

Cited by 66 publications

References 43 publications

Dissipative Particle Dynamics (DPD): An Overview and Recent Developments

Dissipative Particle Dynamics (DPD): An Overview and Recent Developments

Forced transport of deformable containers through narrow constrictions

Single cell rheometry with a microfluidic constriction: Quantitative control of friction and fluid leaks between cell and channel walls

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