Modeling improved production of the chemotherapeutic polypeptide actinomycin D by a novel Streptomyces sp. strain from a Saharan soil

Djinni, Ibtissem; Defant, Andrea; Djoudi, Warda; Chaouch, Faouzia Chaabane; Souagui, Samiha; Kecha, Mouloud; Mancini, Ines

doi:10.1016/j.heliyon.2019.e01695

Cited by 15 publications

(16 citation statements)

References 38 publications

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“…Under the optimized culture conditions, a 58.56% increase of actinomicyn D formation was obtained, arriving at the value of 656.46 mg/L. These results suggested interest in scaling-up the process for access to this molecule, which is currently employed to treat some highly aggressive tumors, alone or in combined chemotherapies [111].…”

Section: Antimicrobialsmentioning

confidence: 83%

“…Compound 47 emerged as the most active in the series, mainly on Gram-positive bacteria and fungi (MICs in the range 1-50 µg/mL corresponding to 4.6-230 µM). [24,81] Valerylpyrrothine (6), Isovalerylpyrrothine (7), Formylpyrrothine (8), Aureothricin (9) antibacterial and antifungal Saccharothrix algeriensis NRRL B-24137, fully sequenced strain Saharan soil [82] Crotonyl-pyrrothine (10), Sorbyl-pyrrothine (11), 2-Hexenyl-pyrrothine (12), 2-Methyl-3-pentenyl-pyrrothine (13) antibacterial and antifungal Saccharothrix algeriensis NRRL B-24137 Saharan soil [83] Benzoyl-pyrrothine dithiolopyrrolone (14) antibacterial, antifungal Saccharothrix algeriensis NRRL B-24137 palm grove soil (Southern Algeria) [84] Mutactimycin PR (15) Toumatia et al [110] and, recently, Djinni et al [111] isolated novel strains producing actinomycin D (49) and showed that genus Streptomyces isolated from Saharan soil of Ain amenas (Streptomyces sp. IA1) and Beni Abbes-Bechar (Streptomyces sp.…”

Section: Antimicrobialsmentioning

confidence: 99%

“…It was first identified from Actinomyces antibioticus in 1940s, later produced by various Streptomyces and Micromonospora species in the world, and also isolated from the Algerian desert soil (Ain Amenas and Beni Abbes)-derived Streptomyces strains Streptomyces sp. IA1 [110,111], demonstrating the effectiveness of the compound for biocontrol against chocolate spots of field bean and Fusarium wilt of flax diseases.…”

Section: Tumor Cells Growth Inhibitorsmentioning

confidence: 99%

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Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

et al. 2019

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Actinobacteria, in particular “rare actinobacteria” isolated from extreme ecosystems, remain the most inexhaustible source of novel antimicrobials, offering a chance to discover new bioactive metabolites. This is the first overview on actinobacteria isolated in Algeria since 2002 to date with the aim to present their potential in producing bioactive secondary metabolites. Twenty-nine new species and one novel genus have been isolated, mainly from the Saharan soil and palm groves, where 37.93% of the most abundant genera belong to Saccharothrix and Actinopolyspora. Several of these strains were found to produce antibiotics and antifungal metabolites, including 17 new molecules among the 50 structures reported, and some of these antibacterial metabolites have shown interesting antitumor activities. A series of approaches used to enhance the production of bioactive compounds is also presented as the manipulation of culture media by both classical methods and modeling designs through statistical strategies and the associations with diverse organisms and strains. Focusing on the Algerian natural sources of antimicrobial metabolites, this work is a representative example of the potential of a closely combined study on biology and chemistry of natural products.

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Section: Antimicrobialsmentioning

confidence: 83%

Section: Antimicrobialsmentioning

confidence: 99%

Section: Tumor Cells Growth Inhibitorsmentioning

confidence: 99%

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Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

et al. 2019

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“…Using MS and NMR techniques, the active compounds were identified as actinomycins X0β, X2, and D [33]. In 2019, a similar report declared the production of actinomycin D by a genus Streptomyces isolated from the Saharan desert [34]. Literature ensures that Actinobacteria in different environments produce Actinomycin D, although in our study, we identified Actinomycin analogs with a high inhibitory effect against MRSA strains produced by desert Actinobacteria.…”

Section: Discussionmentioning

confidence: 55%

Antimicrobial Profile of Actinomycin D Analogs Secreted by Egyptian Desert Streptomyces sp. DH7

et al. 2021

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Egyptian deserts are an underexplored ecological niche, especially the Sinai Peninsula. In our recent study, we explored this extreme environment and shed light on the bioactive capabilities of desert Actinobacteria isolated from Sinai. Fifty desert Actinobacteria were isolated from the Sinai desert using mineral salt media, basal media, and starch casein media. The filtrate of Streptomyces sp. DH 7 displayed a high inhibitory effect against multidrug-resistant Staphylococcus aureus (MRSA) strains. The 16S rDNA sequencing confirmed that isolate DH7 belongs to the genus Streptomyces. The NJ phylogenetic tree showed relatedness to the Streptomyces flavofuscus strain NRRL B-2594 and Streptomyces pratensis strain ch24. The minimum inhibitory concentrations against MRSA were 16 and 32 μg/μL. Chemical investigation of the ethyl acetate extract of Streptomyces sp. DH7 led to the isolation and purification of natural products 1–4. Structure elucidation of the purified compounds was performed using detailed spectroscopic analysis including 1 and 2D NMR, and ESI-MS spectrometry. To the best of our knowledge, this is the first report for the isolation of compounds 1–4 from a natural source, while synthetic analogs were previously reported in the literature. Compounds 3–4 were identified as actinomycin D analogues and this is the first report for the production of actinomycin D analogs from the Sinai desert with an inhibitory effect against MRSA. We indorse further study for this analog that can develop enhanced antimicrobial activities. We confirm that the desert ecosystems in Egypt are rich sources of antibiotic-producing Actinobacteria.

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“…is approach has been successfully applied in secondary metabolites production [20,21] and particularly in enhanced antibiotic production by actinobacteria [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Antibacterial Activity of Paludifilum halophilum and Identification of N-(1-Carboxy-ethyl)-phthalamic Acid as the Main Bioactive Compound

Frikha-Dammak

Fakhfakh

Belhaj

et al. 2020

BioMed Research International

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The aim of this study was to determine the combined effect of fermentation parameters and enhance the production of cellular biomass and antibacterial compounds from Paludifilum halophilum SMBg3 using the response surface methodology (RSM). Eight variables were screened to assess the effects of fermentation parameters on growth and metabolite production by Taguchi experimental design. Among these, the initial pH, temperature, and the percentage of MgSO4·7H2O in the medium were found to be most influential. The Box–Behnken design was applied to derive a statistical model for the optimization of these three fermentation parameters. The optimal parameters were initial pH: 8.3, temperature growth: 44°C, and MgSO4·7H2O: 1.6%, respectively. The maximum yield of biomass and metabolite production were, respectively, 11 mg/mL dry weight and 15.5 mm inhibition zone diameter against Salmonella enterica, which were in agreement with predicted values. The bioactive compounds were separated by the thick-layer chromatography technique and analyzed by GC/MS, NMR (1D and 2D), and Fourier-transform infrared spectroscopy (FT-IR). In addition to several fatty acids, N-(1-carboxy-ethyl)-phthalamic acid was identified as the main antibacterial compound. This element exhibited a potent activity against the ciprofloxacin-resistant Salmonella enterica CIP 8039 and Pseudomonas aeruginosa ATCC 9027 with a minimum inhibitory concentration (MIC) value range of 12.5–25 μg/mL. Results demonstrated that P. halophilum strain SMBg3 is a promising resource for novel antibacterial production due to its high-level yield potential and the capacity for large-scale fermentation.

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Modeling improved production of the chemotherapeutic polypeptide actinomycin D by a novel Streptomyces sp. strain from a Saharan soil

Cited by 15 publications

References 38 publications

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

Antimicrobial Profile of Actinomycin D Analogs Secreted by Egyptian Desert Streptomyces sp. DH7

Enhancement of Antibacterial Activity of Paludifilum halophilum and Identification of N-(1-Carboxy-ethyl)-phthalamic Acid as the Main Bioactive Compound

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