Modeling of Fluorescence Quenching by Lutein in the Plant Light-Harvesting Complex LHCII

Duffy, Christopher D. P.; Chmeliov, Jevgenij; Mačernis, Mindaugas; Šulskus, Juozas; Valkūnas, Leonas; Ruban, Alexander V.

doi:10.1021/jp3110997

Cited by 76 publications

(132 citation statements)

References 85 publications

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“…This can be interpreted as the presence of an equilibrated quencher with a slow variable quenching rate or as the transfer limited population of a strong quencher with inverted transfer kinetics. 10,34,35 Our experiments do not allow to distinguish those possibilities but the broad range of quenching rates and dwell-times up to seconds do suggest that the mechanism is indeed influenced by static disorder. 36 The fact that we observe fluorescence intermittency with a well-defined off-state (dark state) might also be caused by the fact that any underlying distribution of quenching rates that lead to an overall excited state lifetime of less than about 100 ps cannot be resolved in single molecule experiments and will just appear as a dark state.…”

Section: Discussionmentioning

confidence: 78%

Photophysics in single light-harvesting complexes II: from micelle to native nanodisks

et al. 2016

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Most photosynthetic pigment-protein complexes of algae and higher plants are integral membrane proteins and are thus usually isolated in the presence of detergent to provide a hydrophobic interface and prevent aggregation. It was recently shown that the styrene maleic acid (SMA) copolymer can be used instead to solubilize and isolate protein complexes with their native lipid environment into nanodisk particles. We isolated LHCII complexes in SMA and compared their photophysics with trimeric LHCII complexes in β-DM detergent micelles to understand the effect of the native environment on the function of light-harvesting antennae. The triplet state kinetics and the calculated relative absorption cross section of single complexes indicate the successful isolation of trimeric complexes in SMA nanodisks, confirming the trimeric structure as the likely native configuration. The survival time of complexes before they photobleach is increased in SMA compared to detergent which might be explained by a stabilizing effect of the co-purified lipids in nanodisks. We furthermore find an unquenched fluorescence lifetime of 3.5 ns for LHCII in SMA nanodisks which coincides with detergent isolated complexes and notably differs from 2 ns typically found in native thylakoid structures. A large dynamic range of partially quenched complexes both in detergent micelles and lipid nanodisks is demonstrated by correlating the fluorescence lifetime with the intensity and likely reflects the conformational freedom of these complexes. This further supports the hypothesis that fluorescence intermittency is an intrinsic property of LHCII that may be involved in excess energy dissipation in native light-harvesting.

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Section: Discussionmentioning

confidence: 78%

Photophysics in single light-harvesting complexes II: from micelle to native nanodisks

et al. 2016

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“…In the S/F regime, energy delivery to the quencher is slow (B100 ps or even slower), while the dissipation of trapped energy is fast (B10 ps). The slow (20-30 ps) transfer of energy from Chla612 to lutein 620, leading to an effective trapping time of B160 ps, followed by the rapid dissipation of the lutein S 1 excited state due to interconversion to the ground state is an example of the S/F regime 32,33 . The two regimes imply different extents of competition between photochemical and NPQ.…”

Section: Discussionmentioning

confidence: 99%

Economic photoprotection in photosystem II that retains a complete light-harvesting system with slow energy traps

et al. 2014

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The light-harvesting antenna of higher plant photosystem II has an intrinsic capability for self-defence against intense sunlight. The thermal dissipation of excess energy can be measured as the non-photochemical quenching of chlorophyll fluorescence. It has recently been proposed that the transition between the light-harvesting and self-defensive modes is associated with a reorganization of light-harvesting complexes. Here we show that despite structural changes, the photosystem II cross-section does not decrease. Our study reveals that the efficiency of energy trapping by the non-photochemical quencher(s) is lower than the efficiency of energy capture by the reaction centres. Consequently, the photoprotective mechanism works effectively for closed rather than open centres. This type of defence preserves the exceptional efficiency of electron transport in a broad range of light intensities, simultaneously ensuring high photosynthetic productivity and, under hazardous light conditions, sufficient photoprotection for both the reaction centre and the light-harvesting pigments of the antenna.

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“…radical cation formation with chlorophyll (Holt et al, 2005), there are several theories explaining how lutein (and other xanthophylls) can quench excess energy, which include coherent and incoherent energy transfer pathways from chlorophyll to xanthophyll . While there is some evidence showing how zeaxanthin becomes activated as a quencher (Holt et al, 2005;Ahn et al, 2008), there are numerous reports attempting to explain the changes in protein and lutein that point to this pigment as a quencher, as well as modeling work assessing the effectiveness of this quencher in taking excess excitation energy from chlorophyll a Duffy et al, 2013aDuffy et al, , 2013bDuffy et al, , 2014Chmeliov et al, 2015). The formation of quenching chlorophyll-chlorophyll dimers has also been recently advocated .…”

Section: Change: Lhcii Rearrangements/aggregation and The Formation Omentioning

confidence: 99%

Nonphotochemical Chlorophyll Fluorescence Quenching: Mechanism and Effectiveness in Protecting Plants from Photodamage

2016

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We review the mechanism underlying nonphotochemical chlorophyll fluorescence quenching (NPQ) and its role in protecting plants against photoinhibition. This review includes an introduction to this phenomenon, a brief history of major milestones in our understanding of NPQ, definitions, and a discussion of quantitative measurements of NPQ. We discuss the current knowledge and unknown aspects in the NPQ scenario, including the following: DpH, the proton gradient (trigger); lightharvesting complex II (LHCII), PSII light harvesting antenna (site); and changes in the antenna induced by DpH (change), which lead to the creation of the quencher. We conclude that the minimum requirements for NPQ in vivo are DpH, LHCII complexes, and the PsbS protein. We highlight the most important unknown in the NPQ scenario, the mechanism by which PsbS acts upon the LHCII antenna. Finally, we describe a novel, emerging technology for assessing the photoprotective "power" of NPQ and the important findings obtained through this technology.

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Modeling of Fluorescence Quenching by Lutein in the Plant Light-Harvesting Complex LHCII

Cited by 76 publications

References 85 publications

Photophysics in single light-harvesting complexes II: from micelle to native nanodisks

Photophysics in single light-harvesting complexes II: from micelle to native nanodisks

Economic photoprotection in photosystem II that retains a complete light-harvesting system with slow energy traps

Nonphotochemical Chlorophyll Fluorescence Quenching: Mechanism and Effectiveness in Protecting Plants from Photodamage

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