Modeling the coverage of an AFM tip by enzymes and its application in nanobiosensors

Amarante, Adriano Moraes; Oliveira, Guedmiller Souza de; Bueno, Carolina; Cunha, Richard A.; Ierich, Jéssica Cristiane Magalhães; Freitas, Luiz Carlos Gomide; Franca, Eduardo de Faria; Oliveira, Osvaldo N.; Leite, Fábio L.

doi:10.1016/j.jmgm.2014.07.009

Cited by 19 publications

(3 citation statements)

References 29 publications

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“…A possible way to overcome this difficulty is to employ nanobiosensors in which the tip or the cantilever of an AFM is used as the sensing device [11,12]. Moreover, the actual force between the biomolecule and the analyte, for example, a biomarker, can be measured and related to computational simulations using, for example, steered molecular dynamics [15,16]. The AFM approach, sometimes referred to as chemical force spectroscopy, is advantageous for various reasons.…”

Section: Nanobiosensors Can Be Used In the Detection Of Neurologic DImentioning

confidence: 99%

Nanoneurobiophysics: new Challenges for Diagnosis and Therapy of Neurologic Disorders

Leite

Hausen

Oliveira

et al. 2015

Nanomedicine (Lond.)

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Section: Nanobiosensors Can Be Used In the Detection Of Neurologic DImentioning

confidence: 99%

Nanoneurobiophysics: new Challenges for Diagnosis and Therapy of Neurologic Disorders

Leite

Hausen

Oliveira

et al. 2015

Nanomedicine (Lond.)

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“…They can also detect metals, such as mercury, lead, chromium, toxins, and endocrine disrupting chemicals. The detection of persistent pollutants and heavy metals in aqueous samples can be performed with sensors and biosensors, such as an AFM tip nanobiosensor with acetyl-CoA carboxylase, an immunosensor based on a modified carbon printed electrode, an electrochemical sensor based on polymeric electrospun nanofibers of polyamide 6 (PA6)/polypyrrole (PPy) surface-modified with two forms of graphene, a biosensor with double encapsulated algae strains Chlorella vulgaris and Pseudokirchneriella subcapitata alginate beads/silica gel, a biosensor containing recombinant Escherichia coli [ 50 , 51 , 52 , 53 , 54 ]. They can convert the information about the presence of the pollutant into a measurable signal; if it is a biosensor, the element used to detect the analyte is biological.…”

Section: Methodsmentioning

confidence: 99%

Xenobiotics—Division and Methods of Detection: A Review

Štefanac

Grgas

Dragičević

2021

JoX

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Xenobiotics are compounds of synthetic origin, usually used for domestic, agricultural, and industrial purposes; in the environment, they are present in micropollutant concentrations and high concentrations (using ng/L to µg/L units). Xenobiotics can be categorized according to different criteria, including their nature, uses, physical state, and pathophysiological effects. Their impacts on humans and the environment are non-negligible. Prolonged exposure to even low concentrations may have toxic, mutagenic, or teratogenic effects. Wastewater treatment plants that are ineffective at minimizing the release of xenobiotic compounds are one of the main sources of xenobiotics in the environment (e.g., xenobiotic compounds reach the environment, affecting both humans and animals). In order to minimize the negative impacts, various laws and regulations have been adopted in the EU and across the globe, with an emphasis on xenobiotics removal from the environment, in a way that is economically, environmentally, and socially acceptable, and will not result in their accumulation, or creation of compounds that are more harmful. Detection methods allow detecting even small concentrations of xenobiotics in samples, but the problem is the diversity and mix of compounds present in the environment, in which it is not known what their effects are). In this review, the division of xenobiotics and their detection methods will be presented.

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“…Experiments with an AFM tip functionalised with acetyl-CoA carboxylase enzyme (ACC enzyme) made it possible to determine the interaction force between said enzyme and its substrate, the herbicide diclofop [4]. Steered molecular dynamics was used to model the force, which shows a possible way to design nanobiosensors and interpret experimental results.…”

mentioning

confidence: 99%

The characterization and use of nanostructured films in sensing applications

Oliveira

2014

The Tenth International Conference on Advanced Semiconductor Devices and Microsystems

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The control of molecular architectures in nanostructured films holds the promise to revolutionise sensing and biosensing, particularly in clinical diagnosis [1]. Such organized films are suitable for immobilising biomolecules with preserved activity, and allows synergy to be sought among distinct types of materials. Indeed, a large number of organic, inorganic and hybrid materials are exploited in organised films produced with either the Langmuir-Blodgett (LB) or the electrostatic layer-by-layer (LbL) techniques. In biosensing, for instance, nanoparticles, nanotubes, polymers and

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Modeling the coverage of an AFM tip by enzymes and its application in nanobiosensors

Cited by 19 publications

References 29 publications

Nanoneurobiophysics: new Challenges for Diagnosis and Therapy of Neurologic Disorders

Nanoneurobiophysics: new Challenges for Diagnosis and Therapy of Neurologic Disorders

Xenobiotics—Division and Methods of Detection: A Review

The characterization and use of nanostructured films in sensing applications

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