Modeling the distribution of malachite green in zebrafish using matrix-assisted laser desorption/ionization mass spectrometry imaging

He, Muyi; Wang, Xiujuan; Yu, B. X.; Yang, Mei; Deng, Yamei; Liu, Tong; Li, Yinlong; Chen, Fengming; Xu, Bozhou; Xu, Meixia; Zhang, Feng

doi:10.1007/s00216-021-03664-2

Cited by 3 publications

(5 citation statements)

References 44 publications

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“…Applications of this technique range from drug distribution studies to the detection of biomarkers in abnormal tissue. Table-4 and the paragraphs below summarize recent MALDI-TOF applications of chemical imaging or mapping presented in the literature which are related to veterinary science [155][156][157][158][159].…”

Section: Matrix-assisted Laser Desorption Ionizationtime-of-flight Fo...mentioning

confidence: 99%

“…Available at www.veterinaryworld.org/Vol.15/November-2022/16.pdf He et al [156] prepared cryosections of 30 μm thick sections of zebrafish for MALDI imaging experiments in which the distribution of malachite green was determined. Malachite green is used as a fungicide in aquaculture.…”

Section: Matrix-assisted Laser Desorption Ionizationtime-of-flight Fo...mentioning

confidence: 99%

“…Formin-like protein (m/z = 1772), eukaryotic translation factor 4H (m/z = 3462), metalloendopeptidase (m/z = 7018), and histone H4 (m/z = 11325) Bovine follicles and ovary sections were mapped [155] Peak at m/z = 329.20 Da (malachite green) Mapping malachite green (fungicide) in zebrafish tissue [156]…”

Section: Molecules Of Interest Notes Referencesmentioning

confidence: 99%

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Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Thompson

2022

Vet World

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Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS) has become a valuable laboratory tool for rapid diagnostics, research, and exploration in veterinary medicine. While instrument acquisition costs are high for the technology, cost per sample is very low, the method requires minimal sample preparation, and analysis is easily conducted by end-users requiring minimal training. Matrix-assisted laser desorption ionization-time-of-flight MS has found widespread application for the rapid identification of microorganisms, diagnosis of dermatophytes and parasites, protein/lipid profiling, molecular diagnostics, and the technique demonstrates significant promise for 2D chemical mapping of tissue sections collected postmortem. In this review, an overview of the MALDI-TOF technique will be reported and manuscripts outlining current uses of the technology for veterinary science since 2019 will be summarized. The article concludes by discussing gaps in knowledge and areas of future growth.

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Section: Matrix-assisted Laser Desorption Ionizationtime-of-flight Fo...mentioning

confidence: 99%

Section: Matrix-assisted Laser Desorption Ionizationtime-of-flight Fo...mentioning

confidence: 99%

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Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Thompson

2022

Vet World

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“…MSI can perform qualitative, quantitative, and spatial analyses simultaneously without radionuclides or fluorescent labels. Matrix-assisted laser desorption ionization–mass spectrometry imaging (MALDI-MSI) is the most widely used MSI technique, which can realize high-throughput analysis of multiple molecules . In recent years, MALDI-MSI has shown prosperous developments in the field of toxicology research and gained preliminary applications .…”

Section: Introductionmentioning

confidence: 99%

“…Matrix-assisted laser desorption ionization−mass spectrometry imaging (MALDI-MSI) is the most widely used MSI technique, which can realize high-throughput analysis of multiple molecules. 16 In recent years, MALDI-MSI has shown prosperous developments in the field of toxicology research and gained preliminary applications. 17 The sample types include both clinical and nonclinical samples.…”

Section: ■ Introductionmentioning

confidence: 99%

Spatiotemporal Visualization of Paraquat Distribution, Toxicokinetics, and Its Detoxification in Zebrafish Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging

Qin,

Huang,

Feng

et al. 2024

Chem. Res. Toxicol.

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Paraquat is a highly toxic quaternary ammonium herbicide. It can damage the functions of multiple organs and cause irreversible pulmonary fibrosis in the human body. However, the toxicological mechanism of paraquat is not yet fully understood, and due to the lack of specific antidotes, the clinical treatment of paraquat intoxication is still a great medical challenge. In-depth research on its toxicity mechanism, toxicokinetics, and effective antidotes is urgently demanded. A new molecular imaging technique, matrixassisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), can simultaneously achieve quantitative and spatial analysis and offer an alternative, distinct, and useful technique for paraquat intoxication and consequent detoxication. Here, we visualized the spatial-temporal distribution and conducted toxicokinetic research on paraquat in zebrafish by using stable isotope-labeled internal-standard-aided MALDI-MSI for the first time. The results indicated that paraquat had a fast absorption rate and was widely distributed in different organs, such as the brain, gills, kidneys, and liver in zebrafish. Its half-life was long, and the elimination rate was slow. Paraquat reached its peak at 30 min and was mainly distributed in kidneys and intestines and then showed a tendency of declining first but mildly rising later at 6 h, accompanied by a wide distribution in kidneys and intestines again. It suggested that entero-systemic recirculation might lead to the observed secondary peaks, and perhaps it extended the residence time of paraquat in the body. In addition, we validated the potential detoxification effect of sodium salicylate as a potential antidote for paraquat from both the dimensions of distribution and quantification. In conclusion, MALDI-MSI conveniently provided the distinct and quantitative spatial-temporal distribution information on paraquat in the whole body of zebrafish; it will promote the understanding of its toxicokinetic characteristics and provide more valuable information for clinical treatment.

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Malachite green and related substances in environmental samples: Updates on pretreatment and analysis methods

Chen,

He,

Bian

et al. 2024

Journal of Environmental Chemical Engineering

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Modeling the distribution of malachite green in zebrafish using matrix-assisted laser desorption/ionization mass spectrometry imaging

Cited by 3 publications

References 44 publications

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Spatiotemporal Visualization of Paraquat Distribution, Toxicokinetics, and Its Detoxification in Zebrafish Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging

Malachite green and related substances in environmental samples: Updates on pretreatment and analysis methods

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