Modeling the effects of pH variation and bacteriocin synthesis on bacterial growth

Castillo, Benjamín Chacón; Luis, Pastenes; Fernando, Córdova-Lepe

doi:10.1016/j.apm.2022.05.014

Cited by 4 publications

(2 citation statements)

References 57 publications

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“…However, it should be noticed that E. coli and the filamentous fungus usually need different microenvironments, such as pH and oxygen, which will limit their application . For example, cellulase secreted by filamentous fungi generally performs the maximum function at a relatively acidic pH (4.0–5.5), which is not suitable for the growth of E. coli . , Furthermore, filamentous fungi are mostly aerobic, while some reduced chemicals synthesizing E. coli need to be cultivated and fermented under anaerobic conditions. Therefore, studies to achieve the stable coexistence of E. coli with fungus still need to be addressed in the future.…”

Section: Microbial Coculture Of E Coli With Filamentous Fungimentioning

confidence: 99%

Construction of Coculture System Containing Escherichia coli with Different Microbial Species for Biochemical Production

Pan

Yang

et al. 2023

ACS Synth. Biol.

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Microbial synthesis of target chemicals usually involves multienzymatic reactions in vivo, especially for compounds with a long metabolic pathway. However, when various genes are introduced into one single strain, it leads to a heavy metabolic burden. In contrast, the microbial coculture system can allocate metabolic pathways into different hosts, which will relieve the metabolic burdens. Escherichia coli is the most used chassis to synthesize biofuels and chemicals owing to its well-known genetics, high transformation efficiency, and easy cultivation. Accordingly, cocultures containing the cooperative E. coli with other microbial species have received great attention. In this review, the individual applications and boundedness of different combinations will be summarized. Additionally, the strategies for the self-regulation of population composition, which can help enhance the stability of a coculture system, will also be discussed. Finally, perspectives for the cocultures will be proposed.

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Section: Microbial Coculture Of E Coli With Filamentous Fungimentioning

confidence: 99%

Construction of Coculture System Containing Escherichia coli with Different Microbial Species for Biochemical Production

Pan

Yang

et al. 2023

ACS Synth. Biol.

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“…The microenvironment, involving factors such as pH, oxygen utilization, and nutrients, plays a fundamental role in shaping the behavior, growth, and survival of bacteria . Inappropriate pH levels can deactivate enzymes, damage DNA, and denature protein, thereby perturbing metabolic pathways and bacterial physiology , Besides, bacteria evolved a large array of pH regulatory mechanisms that enable them to reprogram behavior in external stimuli . Consequently, monitoring the localized pH of the bacterial microenvironment is vital for comprehending bacterial adaptation and behavior in the presence of chemical or mechanical stimuli. , The developments of localized pH measurement are prosperous, with notable sensors based on nanomaterials, fluorescent pH indicators, and microfabricated electrodes being proposed to improve sensitivity, precision, and real-time monitoring capabilities.…”

Section: Introductionmentioning

confidence: 99%

Real-Time pH Sensor in Bacterial Microenvironments Using Liquid Crystal Core–Shell Microspheres

Xie,

Li,

Lin

et al. 2024

Anal. Chem.

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It is well-known that the bacterial microenvironment imposes restrictions on the growth and behavior of bacteria. The localized monitoring of microenvironmental factors is appreciated when consulting bacterial adaptation and behavior in the presence of chemical or mechanical stimuli. Herein, we developed a novel liquid crystal (LC) biosensor in a microsphere configuration for real-time 3D monitoring of the bacteria microenvironment, which was implemented by a microfluidic chip. As a proof of concept, a LC gel (LC-Gel) microsphere biosensor was prepared and employed in the localized pH changes of bacteria by observing the configuration change of LC under polarized optical microscopy. Briefly, the microsphere biosensor was constructed in core−shell configuration, wherein the core contained LCE7 (a nematic LC) doped with 4pentylbiphenyl-4′-carboxylic acid (PBA), and the shell encapsulated the bacteria. The protonation of carboxyl functional groups of the PBA induced a change in charge density on the surface of LCE7 and the orientation of E7 molecules, resulting in the transitions of the LC nucleus from axial to bipolar. The developed LC-Gel microspheres pH sensor exhibited its dominant performance on localized pH real-time sensing with a resolution of 0.1. An intriguing observation from the prepared pH biosensor was that the diverse bacteria impelled distinct acidifying or alkalizing effects. Overall, the facile LC-Gel microsphere biosensor not only provides a versatile tool for label-free, localized pH monitoring but also opens avenues for investigating the effects of chemical and mechanical stimuli on cellular metabolism within bacterial microenvironments.

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The biochemical behavior and process parameters of U (VI) removal induced by microorganisms isolated from wastewater in decommissioned mining area

Guo,

Wang,

Lei

et al. 2024

J Radioanal Nucl Chem

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Modeling the effects of pH variation and bacteriocin synthesis on bacterial growth

Cited by 4 publications

References 57 publications

Construction of Coculture System Containing Escherichia coli with Different Microbial Species for Biochemical Production

Construction of Coculture System Containing Escherichia coli with Different Microbial Species for Biochemical Production

Real-Time pH Sensor in Bacterial Microenvironments Using Liquid Crystal Core–Shell Microspheres

The biochemical behavior and process parameters of U (VI) removal induced by microorganisms isolated from wastewater in decommissioned mining area

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