Modeling the thermal stability of enzyme-based in vitro diagnostics biosensors

Snyder, Stephen L.; McAuley, Kimberley B.; McLellan, P. James; Brouwer, Eric B.; McCaw, T.

doi:10.1016/j.snb.2011.02.008

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…The results show that the TEMPO-CNC glucose sensor offers better operational stability than the GA sensor (85.3% of the initial current retained after 30 measurements: Figure S5). Ninety percent of original activity is a reasonable threshold for expiration of the sensor; hence these results confirm that the TEMPO-CNC glucose sensor has satisfactory operational stability and can be applied to multi-cycle measurements [50].…”

Section: Operational Stability Of the Tempo-cnc Glucose Sensorsupporting

confidence: 65%

Screen-Printed Glucose Sensors Modified with Cellulose Nanocrystals (CNCs) for Cell Culture Monitoring

et al. 2020

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Glucose sensors are potentially useful tools for monitoring the glucose concentration in cell culture medium. Here, we present a new, low-cost, and reproducible sensor based on a cellulose-based material, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) oxidized-cellulose nanocrystals (CNCs). This novel biocompatible and inert nanomaterial is employed as a polymeric matrix to immobilize and stabilize glucose oxidase in the fabrication of a reproducible, operationally stable, highly selective, cost-effective, screen-printed glucose sensor. The sensors have a linear range of 0.1–2 mM (R2 = 0.999) and a sensitivity of 5.7 ± 0.3 µA cm−2∙mM−1. The limit of detection is 0.004 mM, and the limit of quantification is 0.015 mM. The sensor maintains 92.3 % of the initial current response after 30 consecutive measurements in a 1 mM standard glucose solution, and has a shelf life of 1 month while maintaining high selectivity. We demonstrate the practical application of the sensor by monitoring the glucose consumption of a fibroblast cell culture over the course of several days.

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Section: Operational Stability Of the Tempo-cnc Glucose Sensorsupporting

confidence: 65%

Screen-Printed Glucose Sensors Modified with Cellulose Nanocrystals (CNCs) for Cell Culture Monitoring

et al. 2020

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“…The degradation of test strips under stress condition of temperature have been extensively studied and could be found in the literature [8,9]. However, the present research not only studied the degradation pattern under temperature/humidity stress but also estimated the lifetime of the test strips using a statistical method.…”

Section: Resultsmentioning

confidence: 99%

“…Weibull distribution was applied as the lifetime distribution of the strips in the present study. Lifetime of strips in normal use condition was predicted using the lifetime data obtained from the accelerated degradation tests by adopting a modified Eyring model [7][8][9].…”

Section: Methodsmentioning

confidence: 99%

“…In general, single parameter, i.e. temperature or humidity, as an accelerating parameter is used to assess the accelerated stability of Point-of-Care Testing (POCT) diagnostic devices [6][7][8][9]. In the present research, the degradation model and the lifetime prediction were systematically studied under combined accelerated stress conditions of temperature and humidity as stress factors.…”

Section: Introductionmentioning

confidence: 99%

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Lifetime Assessment of POCT Strips through Accelerated Degradation Test

Choi¹,

Yang²

2015

Pharm Anal Acta

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In general, single parameter, i.e. temperature, as an accelerating parameter is used to assess the accelerated stability of Point-of-Care Testing (POCT) diagnostic devices. However, humidity also plays an important role in deteriorating the strip performance since major components of test strips are proteins such as enzymes. 5 different Temp/Humi conditions were used to assess the lifetime of strips. Degradation of test strips were studied through the accelerated stability test and the lifetime was assessed using commercial POCT products. The failure time of the test strips was determined to be the time when the concentration at the time of the measurement was out of the range of ± 15% of the initial concentration value, by following the degradation pattern in an exponential fashion. For the combined Temp/Humi accelerated conditions, the lifetime prediction was made by adopting a modified Eyring Eq. Model for Stress-Life relationship, and the B10 lifetime (with CL=90%) was estimated to be 505 h (lower limit) when Weibull distribution was applied as the lifetime distribution of strips.

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“…Enzyme biosensors have attracted much attention in recent years due to their potential applications in clinical diagnostics, pharmaceutical, environmental analysis and food industry [1][2][3]. The crucial step in the development of enzyme-based biosensors is the effective immobilization of an enzyme on the electrode surface [4].…”

Section: Introductionmentioning

confidence: 99%