Modelling nematode population growth and damage

Ehwaeti, Mahmoud E.; Elliott, Matthew; McNicol, J.M; Phillips, M. S.

doi:10.1016/s0261-2194(00)00098-3

Cited by 26 publications

(16 citation statements)

References 12 publications

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“…However, there are known to be major physiological differences between M. fallax and M. chitwoodi (Wesemael et al, 2006), so field data on M. fallax may not be applicable to M. chitwoodi. Ehwaeti et al (2000) demonstrated that damage by M. incognita was density dependent and increased with increasing duration of plant growth. This finding is confirmed by our results.…”

Section: Discussionmentioning

confidence: 99%

Quality damage on carrots (Daucus carota L.) caused by the root-knot nematode Meloidogyne chitwoodi

Wesemael

Moens²

2008

Nematol

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In the Belgian provinces Antwerp and Limburg, the root-knot nematode, Meloidogyne chitwoodi, is widespread. These regions are important production areas of carrot, Daucus carota, for the processing industry. Nineteen carrot cultivars were screened for potential resistance for M. chitwoodi. Egg masses of M. chitwoodi were found on all cultivars. However, there were no egg masses on more than 80% of plants of cvs Berlanda, Bolero, Chantenay, Nantucket and Parmex. By contrast, on cvs ABK, Douceur, Maxi and Merida egg masses were formed on all of the tested plants. To gain information about the damage caused by M. chitwoodi, carrots were grown in soil infected with different densities of nematodes. There was no effect of M. chitwoodi on the length, width and weight of the carrot taproot. Damage caused by M. chitwoodi was manifested by severe galling near the lenticels. Inoculation of nematodes 6 weeks after the carrots emerged resulted in a higher percentage of infected carrots and damaged taproots compared with earlier inoculation times. The effect of the time of harvest on nematode infection and damage was examined. When harvested 100 days after sowing in soil with low nematode densities (two second-stage juveniles (J2)/100 g soil), no damage was reported. Harvesting 120 and 140 days after sowing resulted in 10 and 20% damaged carrots, respectively. With initial M. chitwoodi densities of 25 J2/100 g soil, the percentage of damaged taproots increased from 10% when harvested 100 days after sowing to 70% when harvested 140 days after sowing. In a field trial, 11.5% of the carrots were damaged after a field period of 139 days and the initial M. chitwoodi population increased from 3 to 111 J2/100 g soil. It is recommended that growing carrots in M. chitwoodi-infested fields should be avoided. However, damage can be limited in fields with low initial nematode populations when the growing period is reduced.

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Section: Discussionmentioning

confidence: 99%

Quality damage on carrots (Daucus carota L.) caused by the root-knot nematode Meloidogyne chitwoodi

Wesemael

Moens²

2008

Nematol

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“…On the contrary, Arabidopsis thaliana expressing compound dsRNA constructs of housekeeping genes due to crossing of different RNAi lines showed enhanced HIGS efficacy against M. incognita (Charlton et al, 2010). Nevertheless, achieving complete nematode resistance is practically impossible and 60% reduction in nematode multiplication per generation (RKNs complete three generations in an annual host crop) is sufficient to bring down nematode population below the threshold level (Ehwaeti et al, 2000; Fuller et al, 2008). It is to be noted that in our study, the transgenic events, 18.4 ( msp-18 -specific) and 20.3 ( msp-20 -specific) conferred 69.68 and 67.30% resistance to RKN, respectively, in terms of reduced MF.…”

Section: Discussionmentioning

confidence: 99%

Host-Induced Silencing of Two Pharyngeal Gland Genes Conferred Transcriptional Alteration of Cell Wall-Modifying Enzymes of Meloidogyne incognita vis-à-vis Perturbed Nematode Infectivity in Eggplant

et al. 2017

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The complex parasitic strategy of Meloidogyne incognita appears to involve simultaneous expression of its pharyngeal gland-specific effector genes in order to colonize the host plants. Research reports related to effector crosstalk in phytonematodes for successful parasitism of the host tissue is yet underexplored. In view of this, we have used in planta effector screening approach to understand the possible interaction of pioneer genes (msp-18 and msp-20, putatively involved in late and early stage of M. incognita parasitism, respectively) with other unrelated effectors such as cell-wall modifying enzymes (CWMEs) in M. incognita. Host-induced gene silencing (HIGS) strategy was used to generate the transgenic eggplants expressing msp-18 and msp-20, independently. Putative transformants were characterized via qRT-PCR and Southern hybridization assay. SiRNAs specific to msp-18 and msp-20 were also detected in the transformants via Northern hybridization assay. Transgenic expression of the RNAi constructs of msp-18 and msp-20 genes resulted in 43.64–69.68% and 41.74–67.30% reduction in M. incognita multiplication encompassing 6 and 10 events, respectively. Additionally, transcriptional oscillation of CWMEs documented in the penetrating and developing nematodes suggested the possible interaction among CWMEs and pioneer genes. The rapid assimilation of plant-derived carbon by invading nematodes was also demonstrated using 14C isotope probing approach. Our data suggests that HIGS of msp-18 and msp-20, improves nematode resistance in eggplant by affecting the steady-state transcription level of CWME genes in invading nematodes, and safeguard the plant against nematode invasion at very early stage because nematodes may become the recipient of bioactive RNA species during the process of penetration into the plant root.

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“…Temperature and moisture have great infiuence on soil microorganisms, especially in rainfed production systems. Mathemafical models have been derived to anticipate damage and crop loss induced by many plant-parasific nematodes (12,13,41,53). Temperature and precipitafion have previously been used to predict the activity of nematodes in the soil.…”

Section: Discussionmentioning

confidence: 99%

Relationship Between Climatic Factors and Distribution of Pratylenchus spp. in the Dryland Wheat-Production Areas of Eastern Washington

et al. 2013

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Modelling nematode population growth and damage

Cited by 26 publications

References 12 publications

Quality damage on carrots (Daucus carota L.) caused by the root-knot nematode Meloidogyne chitwoodi

Quality damage on carrots (Daucus carota L.) caused by the root-knot nematode Meloidogyne chitwoodi

Host-Induced Silencing of Two Pharyngeal Gland Genes Conferred Transcriptional Alteration of Cell Wall-Modifying Enzymes of Meloidogyne incognita vis-à-vis Perturbed Nematode Infectivity in Eggplant

Relationship Between Climatic Factors and Distribution of Pratylenchus spp. in the Dryland Wheat-Production Areas of Eastern Washington

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