Modelling of oligodextran production via an immobilized enzyme membrane reactor: Bioreaction-separation coupling mechanism

Li, Xianhui; Su, Ziran; Luo, Yusen; Chen, Xueming; Luo, Jianquan; Pinelo, Manuel

doi:10.1016/j.seppur.2021.120024

Cited by 10 publications

(1 citation statement)

References 34 publications

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“…Immobilized enzymes have been developed as affinity separation media for selectively enriching bioactive compounds from complex mixtures [ 12 , 13 , 14 , 15 ]. In recent decades, a number of materials, including magnetic beads [ 16 , 17 , 18 ], microspheres [ 19 , 20 , 21 ], capillary electrophoresis [ 22 ], and MOFs [ 23 ], have been used to immobilize ACE on surfaces and screen ACE inhibitory peptides from protein hydrolysate.…”

Section: Introductionmentioning

confidence: 99%

Anchoring of Polymer Loops on Enzyme-Immobilized Mesoporous ZIF-8 Enhances the Recognition Selectivity of Angiotensin-Converting Enzyme Inhibitory Peptides

et al. 2023

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Immobilized angiotensin-converting enzyme (ACE) is a promising material for the rapid screening of antihypertensive drugs, but the nonspecific adsorption is a serious problem in separation processes involving complex biological products. In this study, triblock copolymers with dopamine (DA) block as anchors and PEG block as the main body (DA-PEGx-DA) were attached to an immobilized ACE (ACE@mZIF-8/PDA, AmZP) surface via the “grafting to” strategy which endowed them with anti-nonspecific adsorption. The influence of DA-PEGx-DA chain length on nonspecific adsorption was confirmed. The excellent specificity and reusability of the obtained ACE@mZIF-8/PDA/DA-PEG5000-DA (AmZPP5000) was validated by screening two known ACE inhibitory peptides Val-Pro-Pro (VPP, competitive inhibitory peptides of ACE) and Gly-Met-Lys-Cys-Ala-Phe (GF-6, noncompetitive inhibitory peptides of ACE) from a mixture containing active and inactive compounds. These results demonstrate that anchored polymer loops are effective for high-recognition selectivity and AmZPP5000 is a promising compound for the efficient separation of ACE inhibitors in biological samples.

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Section: Introductionmentioning

confidence: 99%

Anchoring of Polymer Loops on Enzyme-Immobilized Mesoporous ZIF-8 Enhances the Recognition Selectivity of Angiotensin-Converting Enzyme Inhibitory Peptides

et al. 2023

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Glu@Zn‐Ni MOF Constructed Through Ligand Exchange for Bi‐Enzyme Immobilization With Improved Performance in Rare Ginsenoside Production

Yue,

Li,

Liu

et al. 2024

Applied Organom Chemis

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The biological conversion method using immobilized enzymes has become an important means to produce ginsenosides. Among them, the preprepared Zn‐Ni MOF could be used to immobilize glycosyltransferase by specific adsorption for ginsenoside Rh2 production. However, the low enzyme load of Zn‐Ni MOF limited its wide application. In this study, we successfully synthesized the Glu@Zn‐Ni MOF filled with nanospheres by modifying the previously prepared Zn‐Ni MOF with glutamic acid (Glu) through ligand exchange. This modification significantly increased the specific surface area of the Glu@Zn‐Ni MOF and enhanced the material's enzyme‐carrying capacity. After immobilizing glycosyltransferase (UGT) onto the Glu@Zn‐Ni MOF, the UGT@Glu@Zn‐Ni MOF demonstrated improved stability across different pH levels, temperatures, and repeated cycles. After seven cycles, the immobilized enzyme remained at 83.42% of its initial enzyme activity. To further enhance the process, we incorporated immobilized sucrose synthase into the system to form a coupled reaction. This approach not only reduced substrate costs but also increased the yield of ginsenoside Rh2. By optimizing the coupling reaction through adjustments to the reaction conditions and the addition of suitable surfactants and organic solvents, we achieved a yield of 5.41 μg/mL of Rh2. The development and optimization of this material hold significant potential and value for industrial applications in both material enhancement and ginsenoside synthesis.

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3D printed polylactide scaffolding for laccase immobilization to improve enzyme stability and estrogen removal from wastewater

Rybarczyk

Smułek

Grzywaczyk

et al. 2023

Bioresource Technology

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Modelling of oligodextran production via an immobilized enzyme membrane reactor: Bioreaction-separation coupling mechanism

Cited by 10 publications

References 34 publications

Anchoring of Polymer Loops on Enzyme-Immobilized Mesoporous ZIF-8 Enhances the Recognition Selectivity of Angiotensin-Converting Enzyme Inhibitory Peptides

Anchoring of Polymer Loops on Enzyme-Immobilized Mesoporous ZIF-8 Enhances the Recognition Selectivity of Angiotensin-Converting Enzyme Inhibitory Peptides

Glu@Zn‐Ni MOF Constructed Through Ligand Exchange for Bi‐Enzyme Immobilization With Improved Performance in Rare Ginsenoside Production

3D printed polylactide scaffolding for laccase immobilization to improve enzyme stability and estrogen removal from wastewater

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