Modelling simultaneous anaerobic methane and ammonium removal in a granular sludge reactor

Winkler, Mari‐Karoliina H.; Ettwig, KF; Vannecke, Thomas; Stultiens, Karin; Bogdan, A. V.; Kartal, Boran; Volcke, Eveline

doi:10.1016/j.watres.2015.01.039

Cited by 75 publications

(50 citation statements)

References 33 publications

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“…In this work, the counter‐diffusional supply of CH 4 and liquid substrates (NO 2 − and NH 4 + ) in the MBfR together with the higher NO 2 − affinity of DAMO bacteria (

K_{N O 2}^{D b}

of 0.01 g N m −3 ) than Anammox bacteria (

K_{N O 2}^{A n}

of 0.05 g N m −3 ) led to an abundance of DAMO bacteria relatively comparable with that of Anammox bacteria in the biofilm. In comparison, Winkler et al () reported a competitive advantage of Anammox bacteria over DAMO bacteria in a granular system, which might result from the co‐diffusional supply of substrates (CH 4 , NO 2 − and NH 4 + ) as well as the higher NO 2 − affinity of Anammox bacteria than DAMO bacteria (

K_{N O 2}^{A n}

and

K_{N O 2}^{D b}

are 0.1 g N m −3 and 0.6 g N m −3 , respectively) applied in the model.…”

Section: Resultsmentioning

confidence: 95%

Achieving complete nitrogen removal by coupling nitritation‐anammox and methane‐dependent denitrification: A model‐based study

Chen

Guo

Xie

et al. 2015

Biotech & Bioengineering

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The discovery of denitrifying anaerobic methane oxidation (DAMO) processes enables the complete nitrogen removal from wastewater by utilizing the methane produced on site from anaerobic digesters. This model-based study investigated the mechanisms and operational window for efficient nitrogen removal by coupling nitritation-anaerobic ammonium oxidation (Anammox) and methane-dependent denitrification in membrane biofilm reactors (MBfRs). A mathematical model was applied to describe the microbial interactions among Anammox bacteria, DAMO archaea, and DAMO bacteria. The model sufficiently described the batch experimental data from an MBfR containing an Anammox-DAMO biofilm with different feeding nitrogen compositions, which confirmed the validity of the model. The effects of process parameters on the system performance and microbial community structure could therefore be reliably evaluated. The impacts of nitritation produced NO2(-)/NH4(+) ratio, methane supply, biofilm thickness and total nitrogen (TN) surface loading were comprehensively investigated with the model. Results showed that the optimum NO2(-)/NH4(+) ratio produced from nitritation for the Anammox-DAMO biofilm system was around 1.0 in order to achieve the maximum TN removal (over 99.0%), independent on TN surface loading. The corresponding optimal methane supply increased while the associated methane utilization efficiency decreased with the increase of TN surface loading. The cooperation between DAMO organisms and Anammox bacteria played the key role in the TN removal. Based on these results, the proof-of-concept feasibility of a single-stage MBfR coupling nitritation-Anammox-DAMO for complete nitrogen removal was also tested through integrating the model with ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) processes whilst controlling the dissolved oxygen (DO) concentration in the simulated system. The maximum TN removal was found to be achieved at the bulk DO concentration of around 0.17 g m(-3) under the simulation conditions, with the AOB, Anammox bacteria and DAMO organisms coexisting in the biofilm.

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“…In this work, the counter‐diffusional supply of CH 4 and liquid substrates (NO 2 − and NH 4 + ) in the MBfR together with the higher NO 2 − affinity of DAMO bacteria (

K_{N O 2}^{D b}

of 0.01 g N m −3 ) than Anammox bacteria (

K_{N O 2}^{A n}

K_{N O 2}^{A n}

and

K_{N O 2}^{D b}

are 0.1 g N m −3 and 0.6 g N m −3 , respectively) applied in the model.…”

Section: Resultsmentioning

confidence: 95%

Achieving complete nitrogen removal by coupling nitritation‐anammox and methane‐dependent denitrification: A model‐based study

Chen

Guo

Xie

et al. 2015

Biotech & Bioengineering

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“…According to the literature, it makes sense that we found UMR mussels enhanced Candidatus Brocadia and suppressed NO 2 − reducing-NC10. Perhaps n-damo organisms did not have a favorable niche in mussel bed sediment because biodeposition products created an excess of NH 4 + in sediment porewater (Winkler et al, 2015), or burrowing activity increased oxygen concentrations and made methane oxidation unfavorable (Van Bodegom et al, 2001). Our finding that no-mussel sediment contained three times more ANME-2d in deeper, and presumably anoxic sediment, further suggests that mussels broaden the oxic–anoxic interface niche (Chen & Gu, 2017; Luesken et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment

Black

Chimenti

Just

2017

PeerJ

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Targeted qPCR and non-targeted amplicon sequencing of 16S rRNA genes within sediment layers identified the anaerobic ammonium oxidation (anammox) niche and characterized microbial community changes attributable to freshwater mussels. Anammox bacteria were normally distributed (Shapiro-Wilk normality test, W-statistic =0.954, p = 0.773) between 1 and 15 cm depth and were increased by a factor of 2.2 (p < 0.001) at 3 cm below the water-sediment interface when mussels were present. Amplicon sequencing of sediment at depths relevant to mussel burrowing (3 and 5 cm) showed that mussel presence reduced observed species richness (p = 0.005), Chao1 diversity (p = 0.005), and Shannon diversity (p < 0.001), with more pronounced decreases at 5 cm depth. A non-metric, multidimensional scaling model showed that intersample microbial species diversity varied as a function of mussel presence, indicating that sediment below mussels harbored distinct microbial communities. Mussel presence corresponded with a 4-fold decrease in a majority of operational taxonomic units (OTUs) classified in the phyla Gemmatimonadetes, Actinobacteria, Acidobacteria, Plantomycetes, Chloroflexi, Firmicutes, Crenarcheota, and Verrucomicrobia. 38 OTUs in the phylum Nitrospirae were differentially abundant (p < 0.001) with mussels, resulting in an overall increase from 25% to 35%. Nitrogen (N)-cycle OTUs significantly impacted by mussels belonged to anammmox genus Candidatus Brocadia, ammonium oxidizing bacteria family Nitrosomonadaceae, ammonium oxidizing archaea genus Candidatus Nitrososphaera, nitrite oxidizing bacteria in genus Nitrospira, and nitrate- and nitrite-dependent anaerobic methane oxidizing organisms in the archaeal family “ANME-2d” and bacterial phylum “NC10”, respectively. Nitrosomonadaceae (0.9-fold (p < 0.001)) increased with mussels, while NC10 (2.1-fold (p < 0.001)), ANME-2d (1.8-fold (p < 0.001)), and Candidatus Nitrososphaera (1.5-fold (p < 0.001)) decreased with mussels. Co-occurrence of 2-fold increases in Candidatus Brocadia and Nitrospira in shallow sediments suggests that mussels may enhance microbial niches at the interface of oxic–anoxic conditions, presumably through biodeposition and burrowing. Furthermore, it is likely that the niches of Candidatus Nitrososphaera and nitrite- and nitrate-dependent anaerobic methane oxidizers were suppressed by mussel biodeposition and sediment aeration, as these phylotypes require low ammonium concentrations and anoxic conditions, respectively. As far as we know, this is the first study to characterize freshwater mussel impacts on microbial diversity and the vertical distribution of N-cycle microorganisms in upper Mississippi river sediment. These findings advance our understanding of ecosystem services provided by mussels and their impact on aquatic biogeochemical N-cycling.

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“…Based on the best fitting in Fig. 5a, the apparent methane affinity coefficient (K CH4 ) of the denitrifying methanotrophs was estimated to be 9.8 Ϯ 2.2 M, which is in the range determined for freshwater denitrifying methanotrophic cultures (3 to 92 M) (29,30). The Monod kinetic equation was also applied to describe the relationship between the denitrifying methanotroph-specific activity and the nitrite concentration (Fig.…”

Section: Figmentioning

confidence: 99%

Anaerobic Oxidation of Methane Coupled to Nitrite Reduction by Halophilic Marine NC10 Bacteria

Geng

Cai

et al. 2015

Appl Environ Microbiol

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Anaerobic oxidation of methane (AOM) coupled to nitrite reduction is a novel AOM process that is mediated by denitrifying methanotrophs. To date, enrichments of these denitrifying methanotrophs have been confined to freshwater systems; however, the recent findings of 16S rRNA and pmoA gene sequences in marine sediments suggest a possible occurrence of AOM coupled to nitrite reduction in marine systems. In this research, a marine denitrifying methanotrophic culture was obtained after 20 months of enrichment. Activity testing and quantitative PCR (qPCR) analysis were then conducted and showed that the methane oxidation activity and the number of NC10 bacteria increased correlatively during the enrichment period. 16S rRNA gene sequencing indicated that only bacteria in group A of the NC10 phylum were enriched and responsible for the resulting methane oxidation activity, although a diverse community of NC10 bacteria was harbored in the inoculum. Fluorescence in situ hybridization showed that NC10 bacteria were dominant in the enrichment culture after 20 months. The effect of salinity on the marine denitrifying methanotrophic culture was investigated, and the apparent optimal salinity was 20.5‰, which suggested that halophilic bacterial AOM coupled to nitrite reduction was obtained. Moreover, the apparent substrate affinity coefficients of the halophilic denitrifying methanotrophs were determined to be 9.8 ؎ 2.2 M for methane and 8.7 ؎ 1.5 M for nitrite.A naerobic oxidation of methane (AOM) occurs extensively in natural ecosystems and is a crucial biological sink in the global methane cycle that maintains the balance of greenhouse gas content in the atmosphere (1). To date, five electron acceptors that support AOM, including AOM coupled to sulfate reduction (2), nitrite reduction (3), nitrate reduction (4), iron reduction (5), and manganese reduction (5), have been discovered in natural settings. Moreover, on the basis of bioenergetic calculations, researchers have speculated that several other types of AOM (e.g., AOM coupled to perchlorate reduction, arsenate reduction, and selenate reduction) may exist in nature (6); however, these possible types have not yet been confirmed. In particular, AOM coupled to nitrite reduction has been a predominant research focus in the past several years. AOM coupled to nitrite reduction was also called nitrite-dependent anaerobic methane oxidation (ndamo) in previous reports. It has been demonstrated that AOM coupled to nitrite reduction is mediated by the bacterium "Candidatus Methylomirabilis oxyfera" (denitrifying methanotroph) (7), which is affiliated with the candidate NC10 phylum (8). This candidate division (NC10 phylum) was first defined by classification of environmental sequences retrieved from aquatic microbial formations in flooded caves (9). To date, our knowledge regarding the NC10 phylum has stemmed largely from research into "Ca. Methylomirabilis oxyfera" and "Ca. Methylomirabilis oxyfera"-like bacteria. According to the phylogenetic affiliations of 16S rRNA gene sequ...

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Modelling simultaneous anaerobic methane and ammonium removal in a granular sludge reactor

Cited by 75 publications

References 33 publications

Achieving complete nitrogen removal by coupling nitritation‐anammox and methane‐dependent denitrification: A model‐based study

Achieving complete nitrogen removal by coupling nitritation‐anammox and methane‐dependent denitrification: A model‐based study

Effect of freshwater mussels on the vertical distribution of anaerobic ammonia oxidizers and other nitrogen-transforming microorganisms in upper Mississippi river sediment

Anaerobic Oxidation of Methane Coupled to Nitrite Reduction by Halophilic Marine NC10 Bacteria

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