Modelling the Cell Transmembrane Potential Dependence on the Structure of the Pulsed Magnetic Field Coils

Lučinskis, Audrius; Novickij, Vitalij; Grainys, Audrius; Novickij, Jurij; Tolvaišienė, Sonata

doi:10.5755/j01.eee.20.8.8432

Cited by 18 publications

(11 citation statements)

References 27 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The 8 kV/cm electric field strength ( E ) has been achieved using the 800 V electrical pulse in a 1 mm electrode gap cuvette. The spatial distribution of the magnetic field was evaluated using the finite element method (FEM) analysis in Comsol Multiphysics software (COMSOL, Stockholm, Sweden) 63 64 . The coil was driven by single current pulse described as a time function with the peak amplitude of 10.2 kA and duration of 1.2 ms ( Fig.…”

Section: Methodsmentioning

confidence: 99%

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

Novickij

Grainys

Lastauskienė

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Electroporation is a phenomenon occurring due to exposure of cells to Pulsed Electric Fields (PEF) which leads to increase of membrane permeability. Electroporation is used in medicine, biotechnology, and food processing. Recently, as an alternative to electroporation by PEF, Pulsed ElectroMagnetic Fields (PEMF) application causing similar biological effects was suggested. Since induced electric field in PEMF however is 2–3 magnitudes lower than in PEF electroporation, the membrane permeabilization mechanism remains hypothetical. We have designed pilot experiments where Saccharomyces cerevisiae and Candida lusitaniae cells were subjected to single 100–250 μs electrical pulse of 800 V with and without concomitant delivery of magnetic pulse (3, 6 and 9 T). As expected, after the PEF pulses only the number of Propidium Iodide (PI) fluorescent cells has increased, indicative of membrane permeabilization. We further show that single sub-millisecond magnetic field pulse did not cause detectable poration of yeast. Concomitant exposure of cells to pulsed electric (PEF) and magnetic field (PMF) however resulted in the increased number PI fluorescent cells and reduced viability. Our results show increased membrane permeability by PEF when combined with magnetic field pulse, which can explain electroporation at considerably lower electric field strengths induced by PEMF compared to classical electroporation.

show abstract

Section: Methodsmentioning

confidence: 99%

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

Novickij

Grainys

Lastauskienė

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Šiuo metu manoma, kad impulsinis magnetinis laukas indukuoja elektrinį lauką ir tokiu būdu poliarizuoja ląstelės membraną, kas įtakoja porų formavimą panašiai kaip elektroporacijoje (Kranjc et al, 2016;Towhidi et al, 2012). Tačiau, greitai kintančio magnetinio lauko indukuotas elektrinis laukas yra keliomis eilėmis mažesnis nei elektroporacijai reikalingas elektrinis laukas (Lucinskis et al, 2014;V. Novickij, Dermol, et al, 2017;V.…”

Section: Stiprių Impulsinių Elektromagnetinių Laukų Taikymai Biotechnunclassified

“…arcquenching chambers of highvoltage high-current switches) (Budin et al, 2017) 1,5 kV 70 kA 400 µs 600 µs Ignitronas NL-1057 (Giesselmann, et al, 1993) 25 kV 300 kA 4,5 ms 4 ms NL7703EHV (Kumar, et al, 2018) 50 kV 100 kA 5 µs 15 µs Tačiau, magnetinio lauko generavimui taip pat reikalingi impulsiniai induktoriai (ritės), o lauko homogeniškumas ir atitinkamai poveikio parametrai priklauso nuo ritės geometrijos. Įvertinant, kad biologiniams eksperimentams reikalingas iki kelių ml tūris, geriausia naudoti solenoido tipo daugiasluoksnes rites (Lucinskis et al, 2014). Tokių ričių (1.2 pav.)…”

Section: įTaisai Naudojami Stipraus Magnetinio Lauko Generatoriuoseunclassified

See 1 more Smart Citation

Mikrosekundinių magnetinių impulsų generatorių kūrimas ląstelių plazminės membranos pralaidumui valdyti

Staigvila¹

2020

View full text Add to dashboard Cite

Vadovas doc. dr. Vitalij NOVICKIJ (Vilniaus Gedimino technikos universitetas, elektros ir elektronikos inžinerija-T 001). Vilniaus Gedimino technikos universiteto Elektros ir elektronikos inžinerijos mokslo krypties disertacijos gynimo taryba: Pirmininkas prof. dr. Algirdas BAŠKYS (Vilniaus Gedimino technikos universitetas, elektros ir elektronikos inžinerija-T 001). Nariai: dr. Matej REBERŠEK (Liublianos universitetas, Slovėnija, elektros ir elektronikos inžinerija-T 001), prof. dr. Artūras SERACKIS (Vilniaus Gedimino technikos universitetas, elektros ir elektronikos inžinerija-T 001), doc. dr. Dainius UDRIS (Vilniaus Gedimino technikos universitetas, elektros ir elektronikos inžinerija-T 001), prof. dr. Algimantas VALINEVIČIUS (Kauno technologijos universitetas, elektros ir elektronikos inžinerija-T 001). Disertacija bus ginama viešame Elektros ir elektronikos inžinerijos mokslo krypties disertacijos gynimo tarybos posėdyje 2021 m. sausio 7 d. 10 val. Vilniaus Gedimino technikos universiteto senato posėdžių salėje.

show abstract

“…The high voltage generators are required for the high intensity electric field generation, which induces reversible and non-reversible cell permeability increase effects [4]- [6]. As a rule a controlled discharge of a capacitor array through the biological load using a pulse forming switch is applied [7].…”

Section: Introductionmentioning

confidence: 99%

Microsecond Electroporator Optimization for Parasitic Load Handling and Damping

Novickij

Stankevič²,

Grainys³

et al. 2015

ElAEE

View full text Add to dashboard Cite

During development of the pulsed power generators the occurrence of the parasitic elements in the circuit is inevitable, which leads to appearance of overvoltage, overcurrent and waveform distortions. This work is focused on the optimization of the microsecond electroporator to enable handling and damping of the parasitic loads. The optimization is based on a flexible PSPICE model of the electroporator, which is used for the compensation of the parasitic parameters. Based on the modelling results the parameters and the circuit elements for the device are selected. The compliance of the prototype's experimental and the PSPICE simulated output pulses is analysed. The optimized circuit of the microsecond electroporator is designed. The system supports current handling up to 100 A and capable to generate up to 4 kV square wave pulses.

show abstract

Modelling the Cell Transmembrane Potential Dependence on the Structure of the Pulsed Magnetic Field Coils

Cited by 18 publications

References 27 publications

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

Mikrosekundinių magnetinių impulsų generatorių kūrimas ląstelių plazminės membranos pralaidumui valdyti

Microsecond Electroporator Optimization for Parasitic Load Handling and Damping

Contact Info

Product

Resources

About