High power pulsed magnetic field generator for application in the bioelectronics area has been developed. The developed magnetic field facility is capable of generating pulsed magnetic field up to 5 T with pulse width in the microsecond range. The maximum repetitive frequency of the pulses is 40 Hz. The generator has been implemented by application of three high power thyristor switches, capacitor bank, specifically designed load coil with crowbar diode and the control circuitry. The inductive coil that has an integrated cuvette with effective volume of 2.4 µl for biological samples is presented in the study. Finite element analysis of the generated pulsed magnetic field and comparison to the acquired experimental pulse are performed. Experimental results of human lymphocyte cells treated by pulsed magnetic field and potential applications of the generator in the bioelectronics area of magnetoporation are discussed. Based on the results further development and improvement ways of the facility for better integration in the area of bioelectronics are proposed.

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During high power pulsed magnetic field treatment of biological samples the cells are subjected to both the high magnetic and induced electric fields. The extent of the influence of each treatment component is poorly studied. The work presents the finite element method analysis of pulsed inductive coils that are used for generation of pulsed magnetic and induced electric fields. The simulated coils, electrical parameters and the output characteristics are evaluated in respect to the induced cell transmembrane potential. The model of the Jurkat T lymphocyte cells is introduced in the analysis. The study includes finite element method analysis of four solenoid coils with different structure and inductance in the range of 2.8 μH to 62 μH. Pulsed magnetic field amplitudes up to 5 T are investigated in this work.

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