Modelling the metabolic shift of polyphosphate-accumulating organisms

Acevedo, B.; Borrás, L.; Oehmen, Adrian; Barat, R.

doi:10.1016/j.watres.2014.07.028

Cited by 21 publications

(8 citation statements)

References 23 publications

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“…This result is significant because only six of the treatment works they screened were configured for EBPR, suggesting that the potential for effective EPBR is more driven by local environmental conditions than by specific "engineered" microbial communities. Carvalheira et al (2014) and Acevedo et al (2014) support this by showing PAO adapt their metabolic processes to optimize substrate utilization, making them more resilient than previously believed.…”

Section: Traditional Ebpr Systems and Recent Enhancementsmentioning

confidence: 60%

“…and it has since been shown that PAO are capable of utilizing both the TCA cycle and Glycolysis (Zhou et al, 2009). Some have suggested that some PAO may switch between metabolic pathways according to internal or external conditions (Acevedo et al, 2012(Acevedo et al, , 2014Majed et al, 2012;Lanham et al, 2013). Utilisation of glycolysis has been shown to yield more efficient P-removal rates than the TCA cycle, within the context of an enhanced CAS system (Lanham et al, 2013).…”

Section: Metabolism Of Paomentioning

confidence: 99%

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A Review of Phosphorus Removal Technologies and Their Applicability to Small-Scale Domestic Wastewater Treatment Systems

Bunce

Ndam

Ofiţeru

et al. 2018

Front. Environ. Sci.

401

204

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The removal of phosphorus (P) from domestic wastewater is primarily to reduce the potential for eutrophication in receiving waters, and is mandated and common in many countries. However, most P-removal technologies have been developed for use at larger wastewater treatment plants that have economies-of-scale, rigorous monitoring, and in-house operating expertise. Smaller treatment plants often do not have these luxuries, which is problematic because there is concern that P releases from small treatment systems may have greater environmental impact than previously believed. Here P-removal technologies are reviewed with the goal of determining which treatment options are amenable to small-scale applications. Significant progress has been made in developing some technologies for small-scale application, namely sorptive media. However, as this review shows, there is a shortage of treatment technologies for P-removal at smaller scales, particularly sustainable and reliable options that demand minimal operating and maintenance expertise or are suited to northern latitudes. In view of emerging regulatory pressure, investment should be made in developing new or adapting existing P-removal technologies, specifically for implementation at small-scale treatment works.

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Section: Traditional Ebpr Systems and Recent Enhancementsmentioning

confidence: 60%

Section: Metabolism Of Paomentioning

confidence: 99%

A Review of Phosphorus Removal Technologies and Their Applicability to Small-Scale Domestic Wastewater Treatment Systems

Bunce

Ndam

Ofiţeru

et al. 2018

Front. Environ. Sci.

401

204

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“…Although the input space was sampled to conform to the assumptions of the metabolic model, i.e., within a narrow range of temperatures, pH and an influent mixture of HAc and HPr, the predictions may not be valid in situations where the PAO population is subject to limiting conditions for extended periods of time. For instance, PAO have been found to shift from PHA to glycogen accumulation in anaerobic conditions following a lack of PO 4 in the influent (Acevedo et al, 2014, 2017)—a phenotypic behavior characteristic of their GAO competitors. Similarly, PAO have been shown to employ the tricarboxylic acid (TCA) cycle to supplement the production of reducing agents to cope with a lack of VFA in the influent, or due to glycogen depletion, possibly as a result of prolonged periods of cellular maintenance (Lanham et al, 2013, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, the application of advanced molecular techniques has led to a more comprehensive understanding of both the metabolic activity and phylogenetic diversity (Oehmen et al, 2007), e.g., differing capacities of PAO clades for denitrification (Zeng et al, 2003a; Flowers et al, 2009; Oehmen et al, 2010b), utilization of the TCA cycle to supply reducing power for PHA formation (Lanham et al, 2014) or shift to glycolysis-driven VFA uptake (Acevedo et al, 2014). Consequently, the complexity of metabolic models, in terms of stoichiometric and kinetic descriptions of the underlying processes, as well as the number of different organisms to account for, has been on the rise.…”

Section: Introductionmentioning

confidence: 99%

Global Sensitivity Analysis of Metabolic Models for Phosphorus Accumulating Organisms in Enhanced Biological Phosphorus Removal

Quang

Rogers

Hofman

et al. 2019

Front. Bioeng. Biotechnol.

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The aim of this study was to identify, quantify and prioritize for the first time the sources of uncertainty in a mechanistic model describing the anaerobic-aerobic metabolism of phosphorus accumulating organisms (PAO) in enhanced biological phosphorus removal (EBPR) systems. These wastewater treatment systems play an important role in preventing eutrophication and metabolic models provide an advanced tool for improving their stability via system design, monitoring and prediction. To this end, a global sensitivity analysis was conducted using standard regression coefficients and Sobol sensitivity indices, taking into account the effect of 39 input parameters on 10 output variables. Input uncertainty was characterized with data in the literature and propagated to the output using the Monte Carlo method. The low degree of linearity between input parameters and model outputs showed that model simplification by linearization can be pursued only in very well defined circumstances. Differences between first and total-order sensitivity indices showed that variance in model predictions was due to interactions between combinations of inputs, as opposed to the direct effect of individual inputs. The major sources of uncertainty affecting the prediction of liquid phase concentrations, as well as intra-cellular glycogen and poly-phosphate was due to 64% of the input parameters. In contrast, the contribution to variance in intra-cellular PHA constituents was uniformly distributed among all inputs. In addition to the intra-cellular biomass constituents, notably PHB, PH2MV and glycogen, uncertainty with respect to input parameters directly related to anaerobic propionate uptake, aerobic poly-phosphate formation, glycogen formation and temperature contributed most to the variance of all model outputs. Based on the distribution of total-order sensitivities, characterization of the influent stream and intra-cellular fractions of PHA can be expected to significantly improve model reliability. The variance of EBPR metabolic model predictions was quantified. The means to account for this variance, with respect to each quantity of interest, given knowledge of the corresponding input uncertainties, was prescribed. On this basis, possible avenues and pre-requisite requirements to simplify EBPR metabolic models for PAO, both structurally via linearization, as well as by reduction of the number of non-influential variables were outlined.

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“…The bacterial population plays a very important part of the WWT community since they remove most of the BOD by metabolizing or degrading the organic matter. Furthermore, In particular, some bacteria are polyphosphate accumulating organisms (PAO) (Acevedo et al, 2014) responsible for most of the phosphate removal, and ammonia-oxidizing bacteria (AOB) that remove ammonia that is toxic to biota downstream ( Zhang et al, 2011). These groups remove contaminants in different ways, PAOs are responsible for removing phosphate by physically storing them in their cells (T. Zhang et al, 2011), while ammonia is removed chemically by AOBs and nitrite-oxidizing bacteria (NOBs) in a two-step process (Acevedo et al, 2014).…”

Section: Wastewater Treatment Communities (Wwtcs)mentioning

confidence: 99%

Effects of ibuprofen and tetracyline on extracellular polymer production

Hanna¹

2021

Preprint

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Extracellular polymer substances (EPS) comprised of proteins, carbohydrates, and other biomolecules, are important for microbiological communities. The EPS provides benefits to wastewater treatment communities (WWTC) such as antibiotic resistance, and community structure. Therefore EPS can be viewed as a valuable health marker of WWTC and therefore it is important to determine effects of contaminants entering wastewater treatment plants (WWTPs) on EPS. WWTC were exposed to the non‐steroidal anti‐inflammatory drug (NSAID) Ibuprofen at 16 ng/mL and 100 ng/mL, and the antibiotic tetracycline at 50 ng/mL to determine how EPS and growth were affected. EPS was analyzed using high performance liquid chromatography (HPLC), Bradford protein analysis, and Anthrone carbohydrate analysis. It was determined that EPS content was lower at both Ibuprofen concentrations, enhanced with tetracycline, and comparable to control when both were present. Changes could not be linked to protein or carbohydrate concentration. Growth inhibition occurred in the presence of both drugs but not each individually, suggesting that Ibuprofen may increase tetracycline sensitivity through EPS inhibition.

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Modelling the metabolic shift of polyphosphate-accumulating organisms

Cited by 21 publications

References 23 publications

A Review of Phosphorus Removal Technologies and Their Applicability to Small-Scale Domestic Wastewater Treatment Systems

A Review of Phosphorus Removal Technologies and Their Applicability to Small-Scale Domestic Wastewater Treatment Systems

Global Sensitivity Analysis of Metabolic Models for Phosphorus Accumulating Organisms in Enhanced Biological Phosphorus Removal

Effects of ibuprofen and tetracyline on extracellular polymer production

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