Knitted fabrics have excellent comfort properties because of their typical porous structure. Different comfort properties of knitted fabrics such as air permeability, thermal absorptivity, and thermal conductivity depend on the properties of raw material and knitting parameters. In this paper, an investigation was done to observe the effect of yarn count, loop length, knitting speed, and yarn input tension in the presence of two uncontrollable noise factors on selected comfort properties of single jersey and 1×1 rib knitted fabrics using the Taguchi experimental design. The results show that yarn count and loop length have significant influence on the thermo-physiological comfort properties of knitted fabrics.