Models of human psoriasis: Zebrafish the newly appointed player

Martínez-Navarro, Francisco J.; Martı́nez-Menchón, Teresa; Mulero, Victoriano; Galindo-Villegas, Jorge

doi:10.1016/j.dci.2019.03.018

Cited by 25 publications

(7 citation statements)

References 101 publications

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“…For both mammals and fish, the skin consists of three layers, epidermis, dermis and hypodermis, and although subtle differences are noted, they broadly function similarly (Le Guellec, Morvan‐Dubois, & Sire, ). This has led to fish skin being used as a research model for human medical problems, such as for the immune disease, psoriasis (Martinez‐Navarro, Martinez‐Menchon, Mulero, & Galindo‐Villegas, ) and for wound healing (Richardson et al, ). Skin is also an important subject for many fish disciplines.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Katzenback

Kellendonk

et al. 2019

Journal of Fish Diseases

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A walleye dermal fibroblastoid cell line, WE‐skin11f, was established and characterized. WE‐skin11f was immunocytochemically positive for two known dermal fibroblast protein markers: vimentin and collagen I. At passage 26, WE‐skin11f cultures contained both diploid and aneuploid populations. Ascorbic acid was required to produce extracellular collagen I fibres. Both of the skin fibroblastoid cell lines, WE‐skin11f and rainbow trout‐derived RTHDF, were not as good as the walleye caudal fin fibroblastoid cell line, WE‐cfin11f, at forming abundant dense extracellular collagen matrices. The thermobiology of WE‐skin11f was similar to that of other walleye cell lines with 26°C showing best temperature for growth and 4°C showing no growth but 100% viability. The transcript levels of b2m and mhIa genes of the major histocompatibility class I receptor in WE‐skin11f were largely similar at all temperatures examined (4, 14, 20 and 26°C). Cortisol had a variety of effects on WE‐skin11f cells: growth inhibition, morphological change from fibroblastoid to epithelioid, and enhancement of barrier function. Treatment of WE‐skin11f cells with the physiologically relevant concentration of 100 ng/ml cortisol inhibited collagen I synthesis and matrix formation. Thus, WE‐skin11f cell line could be useful in fish dermatology, endocrinology, and immunology research.

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Section: Introductionmentioning

confidence: 99%

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Katzenback

Kellendonk

et al. 2019

Journal of Fish Diseases

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“…were unable to promote phagocytosis and the neutrophil chemotaxis, respectively, which are two key features linked to the inflammatory response in zebrafish larvae (89,90). Emerging evidence indicates that in newly hatched zebrafish the skin keratinocytes together with neutrophils have a crucial impact on the production of inflammatory mediators (91,92).…”

Section: Discussionmentioning

confidence: 99%

Pseudozyma Priming Influences Expression of Genes Involved in Metabolic Pathways and Immunity in Zebrafish Larvae

et al. 2020

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Fungi, particularly yeasts, are known essential components of the host microbiota but their functional relevance in development of immunity and physiological processes of fish remains to be elucidated. In this study, we used a transcriptomic approach and a germ-free (GF) fish model to determine the response of newly hatched zebrafish larvae after 24 h exposure to Pseudozyma sp. when compared to conventionally-raised (CR) larvae. We observed 59 differentially expressed genes in Pseudozyma-exposed GF zebrafish larvae compared to their naïve control siblings. Surprisingly, in CR larvae, there was not a clear transcriptome difference between Pseudozyma-exposed and control larvae. Differentially expressed genes in GF larvae were involved in host metabolic pathways, mainly peroxisome proliferator-activated receptors, steroid hormone biosynthesis, drug metabolism and bile acid biosynthesis. We also observed a significant change in the transcript levels of immune-related genes, namely complement component 3a, galectin 2b, ubiquitin specific peptidase 21, and aquaporins. Nevertheless, we did not observe any significant response at the cellular level, since there were no differences between neutrophil migration or proliferation between control and yeast-exposed GF larvae. Our findings reveal that exposure to Pseudozyma sp. may affect metabolic pathways and immune-related processes in germ-free zebrafish, suggesting that commensal yeast likely play a significant part in the early development of fish larvae.

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“…The aforementioned similarities of the zebrafish integument structure together with those of the inflammation mechanisms, make this teleost a fundamental and cost-effective model also to study major dermatologic inflammatory diseases, such as psoriasis. Several models, including mutant, morphant and environmentally inducible models, were created to investigate genetic alterations and molecular mechanisms of psoriasis [ 144 – 152 ].…”

Section: Zebrafish As a Model System In Oncologymentioning

confidence: 99%

The Zebrafish model in dermatology: an update for clinicians

et al. 2022

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Recently, the zebrafish has been established as one of the most important model organisms for medical research. Several studies have proved that there is a high level of similarity between human and zebrafish genomes, which encourages the use of zebrafish as a model for understanding human genetic disorders, including cancer. Interestingly, zebrafish skin shows several similarities to human skin, suggesting that this model organism is particularly suitable for the study of neoplastic and inflammatory skin disorders. This paper appraises the specific characteristics of zebrafish skin and describes the major applications of the zebrafish model in dermatological research.

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Models of human psoriasis: Zebrafish the newly appointed player

Cited by 25 publications

References 101 publications

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Characterization of the continuous skin fibroblastoid cell line, WE‐skin11f, from walleye (Sander vitreus)

Pseudozyma Priming Influences Expression of Genes Involved in Metabolic Pathways and Immunity in Zebrafish Larvae

The Zebrafish model in dermatology: an update for clinicians

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