Moderately Elevated Temperature Eliminates Resistance of Rice Plants with Enhanced Expression of Glutathione Reductase to Intensive Photooxidative Stress

Kouřil, Roman; Lazár, Dušan; Lee, H.; Jo, Jae Cheol; Nauš, Jan

doi:10.1023/b:phot.0000027522.35486.f6

Cited by 23 publications

(8 citation statements)

References 39 publications

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“…It is reported that, with the increase of GR activity, the levels of both total and reduced glutathione showed a tendency to increase (Foyer et al 1995). A number of previous studies has also shown that overexpression of GR lead to an increase in cellular GSH levels and thus enhanced stress tolerance in various plant species (Foyer et al 1995, Pilon-Smits et al 2000, Kouřil et al 2003, Chen et al 2007). Thus, a high activity of GR is necessary for plants to maintain high levels of GSH, especially under stress conditions.…”

Section: Introductionmentioning

confidence: 97%

High level of reduced glutathione contributes to detoxification of lipid peroxide‐derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress

Yin

Mano

Tanaka

et al. 2017

Physiologia Plantarum

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Lipid peroxide-derived reactive carbonyl species (RCS), generated downstream of reactive oxygen species (ROS), are critical damage-inducing species in plant aluminum (Al) toxicity. In mammals, RCS are scavenged primarily by glutathione (reduced form of glutathione, GSH), but in plant Al stress, contribution of GSH to RCS detoxification has not been evaluated. In this study, Arabidopsis plants overexpressing the gene AtGR1 (accession code At3g24170), encoding glutathione reductase (GR), were generated, and their performance under Al stress was examined. These transgenic plants (GR-OE plants) showed higher GSH levels and GSH/GSSG (oxidized form of GSH) ratio, and an improved Al tolerance as they suffered less inhibition of root growth than wild-type under Al stress. Exogenous application of 4-hydroxy-2-nonenal, an RCS responsible for Al toxicity in roots, markedly inhibited root growth in wild-type plants. GR-OE plants suffered significantly smaller inhibition, indicating that the enhanced GSH level increased the capacity of RCS detoxification. The generation of H O due to Al stress in GR-OE plants was lower by 26% than in wild-type. Levels of various RCS, such as malondialdehyde, butyraldehyde, phenylacetaldehyde, (E)-2-heptenal and n-octanal, were suppressed by more than 50%. These results indicate that high levels of GSH and GSH/GSSG ratio by GR overexpression contributed to the suppression of not only ROS, but also RCS. Thus, the maintenance of GSH level by overexpressing GR reinforces dual detoxification functions in plants and is an efficient approach to enhance Al tolerance.

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Section: Introductionmentioning

confidence: 97%

High level of reduced glutathione contributes to detoxification of lipid peroxide‐derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress

Yin

Mano

Tanaka

et al. 2017

Physiologia Plantarum

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“…High‐temperature tolerant rice plants have also been produced by over‐expressing either heat‐shock proteins (Katiyar‐Aggarwal et al. , 2003; Murakami et al , 2004) or enzymes involved in oxidative‐stress tolerance (Kishitani et al , 2000; Kouril et al , 2003) (Table 4).…”

Section: Rice Transformationmentioning

confidence: 99%

“…A heat stress transcription factor (Spl7) has been cloned from rice and its over-expression in spl7 mutant suppressed the leaf-spot development induced by high temperature (Yamanouchi et al, 2002). High-temperature tolerant rice plants have also been produced by over-expressing either heat-shock proteins (Katiyar-Aggarwal et al, 2003;Murakami et al, 2004) or enzymes involved in oxidativestress tolerance (Kishitani et al, 2000;Kouril et al, 2003) ( Table 4).…”

Section: Tolerance To Extreme Temperaturesmentioning

confidence: 99%

Recent advances in rice biotechnology—towards genetically superior transgenic rice

Bajaj

Mohanty

2005

Plant Biotechnology Journal

215

109

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SummaryRice biotechnology has made rapid advances since the first transgenic rice plants were produced 15 years ago. Over the past decade, this progress has resulted in the development of high frequency, routine and reproducible genetic transformation protocols for rice. This technology has been applied to produce rice plants that withstand several abiotic stresses, as well as to gain tolerance against various pests and diseases. In addition, quality improving and increased nutritional value traits have also been introduced into rice. Most of these gains were not possible through conventional breeding technologies. Transgenic rice system has been used to understand the process of transformation itself, the integration pattern of transgene as well as to modulate gene expression. Field trials of transgenic rice, especially insect-resistant rice, have recently been performed and several other studies that are prerequisite for safe release of transgenic crops have been initiated. New molecular improvisations such as inducible expression of transgene and selectable marker-free technology will help in producing superior transgenic product. It is also a step towards alleviating public concerns relating to issues of transgenic technology and to gain regulatory approval. Knowledge gained from rice can also be applied to improve other cereals. The completion of the rice genome sequencing together with a rich collection of full-length cDNA resources has opened up a plethora of opportunities, paving the way to integrate data from the large-scale projects to solve specific biological problems.

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“…It also diminishes the number of oxygen-evolving complexes (Bazzaz and Govindjee 1974, Vassilev et al 2004, Janeczko et al 2005, while on the acceptor site, it inhibits electron transfer from QAto QB - (Sigfridsson et al 2004). Adverse effects of Cd are also visible in carboxylation phase of photosynthesis (Krantev et al 2008). Stomatal conductance and index, transpiration, and net photosynthetic rate are affected, as well as enzymes of dark phase of photosynthesis (Rivera-Becerril et al 2002, Burzyński andŻurek 2007).…”

Section: Introductionmentioning

confidence: 99%

“…The parameters of fluorescence of Chl a (CF) provide in vivo assessment of the photosynthetic apparatus activity under stress. The measurements are noninvasive, and often used as an alternative for gas-exchange measurement of photosynthesis (Kouřil et al 2003, Sitko et al 2017. CF parameters are widely used in ecophysiological and agricultural studies (Sofo et al 2009, Murchie andLawson 2013).…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic response of cabbage in cadmium-spiked soil

Bączek-Kwinta¹,

Juzoń²,

Borek³

et al. 2019

Photosynt.

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Efficiency of a photosynthetic apparatus in plants growing in cadmium-spiked soil as well as plant biomass and cadmium accumulation were assessed in two cabbage cultivars (early and late) in a long-term pot experiment. Cadmium concentrations were 10 and 40 mg(Cd) per kg of dry mass of soil. The early cultivar showed a dose-dependent and stable decrease in SPAD index, while in plants of the late cultivar the response was reversible and less pronounced. A huge drop in the effective quantum yield of PSII photochemistry, photochemical quenching, nonphotochemical quenching, and electron transport rate in the third week of vegetation indicated susceptibility of the early cultivar to cadmium, contrary to the late one. Fresh mass of plants of the early cultivar was diminished by both doses of Cd. For the late cultivar, there was no change in these parameters, indicating its possible usability for phytoremediation, provided the reclaimed area is fenced and protected. Particular attention should also be paid towards late cultivars cabbage farming in areas with a high Cd content. On the other hand, the early cultivar may serve as a bioindicator of Cd-contamination. transport rate; F0 -minimal fluorescence yield at the dark-adapted state; FM -fresh mass; Fv/Fm -maximal quantum yield of PSII photochemistry; NPQ -Stern-Volmer nonphotochemical quenching coefficient; qP -photochemical quenching coefficient; SD -standard deviation; ФPSII -effective quantum yield of PSII photochemistry. Acknowledgements: The study was supported by European Union from European Regional Development Fund in a framework of the Innovative Economy Operational Programme 2007-2013, project ʻThe exploitation of white cabbage for phytoremediation and biofumigation of soils (AGROBIOKAP)ʼ, and DS 3163 research programme of University of Agriculture in Krakow.

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Moderately Elevated Temperature Eliminates Resistance of Rice Plants with Enhanced Expression of Glutathione Reductase to Intensive Photooxidative Stress

Cited by 23 publications

References 39 publications

High level of reduced glutathione contributes to detoxification of lipid peroxide‐derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress

High level of reduced glutathione contributes to detoxification of lipid peroxide‐derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress

Recent advances in rice biotechnology—towards genetically superior transgenic rice

Photosynthetic response of cabbage in cadmium-spiked soil

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