Moderation of morphogenetic and oxidative stress responses in flax in vitro cultures by hydroxynonenal and desferrioxamine

Current Issues in Plant Cryopreservation

“…The addition of desferrioxamine to rice cells during cryopreservation showed a decreased recovery period after cryopreservation (Benson et al, 1995). Detection of methane formation was delayed in flax tissue when exposed to desferrioxamine (Obert et al, 2005), indicating a delayed production of hydroxyl radicals.…”

“…The addition of specific chelating agents (such as desferrioxamine) has been shown to reduce the levels of iron in cryopreserved tissues, with subsequent decreased levels of the hydroxyl radical observed (Benson et al, 1995;Fleck et al, 2000;Obert et al, 2005).…”

“…Identifying the formation of MDA and HNE is commonly used in cryopreservation as an indicator of oxidative stress. High levels of MDA or HNE detected have correlated with decreased survival rates in rice cell suspensions, olive somatic embryos, flax and blackberry shoot tips (Benson et al, 1992;Obert et al, 2005;Uchendu et al, 2010;Lynch et al, 2011).…”

“…The formation of methane when DMSO interacts with the hydroxyl radical can be measured if the sample is placed in an airtight container, with the levels of methane detected correlating with the formation of the hydroxyl radical. This technique has been used as a measurement of oxidative stress in multiple different plant species, such as rice, cocoa, Daucus carota and flax (Benson & Withers 1987;Benson et al, 1995;Obert et al, 2005;Fang et al, 2008). The production of methane is particularly strong during the initial phase of recovery, where it is predicted that antioxidant activity and production is reduced, resulting in increased ROS (Fang et al, 2008).…”

Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer

“…Signalling mediated by ROS involves G-proteins, MAP kinases and protein Tyr phosphatases (Zhang et al 2006, Shao et al 2008. In addition to concentration, the balance between their positive and negative effects is influenced by the physiological and developmental status of tissues (Obert et al 2005).…”