The quantitative research method was used in this study, in which the relationships between the career satisfaction, which includes the employee's satisfaction when evaluating the gains that the employee has achieved in his career, and the intrinsic and extrinsic motivation that provides the employee's motivation for his job, within the framework of organizational values. Primary data were collected from employees of two large enterprises from the public and private sectors. Data were collected with the questionnaire, which is the most frequently used data collection tool in social sciences. There are many factors that are effective in career satisfaction and motivation to continue a career in the same business. In this study, the values of the organization, which is one of these factors, were examined. The data obtained from a sample of 604 people were analyzed through the SPSS program. According to the analysis results, organizational values are positively associated with career satisfaction and motivation. Organizational values explain both career satisfaction and motivation positively and significantly. In addition, it has been determined that the career satisfaction and motivation values of the employees in the public and private sectors are different from each other. It has been observed that there are differences in career satisfaction and motivation values according to the demographic characteristics of the employees.