Modern Blood Banking and Transfusion Practices

Barnes, Asa

doi:10.1093/ajcp/93.5.719

Cited by 14 publications

(26 citation statements)

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“…A decrease in pH is associated with a loss of platelet viability. When the pH drops below 6.2, the platelets will swell and transform from a disc shape to a spherical shape (Harmening, 2012). The morphology of platelets that become round and then dendritic generally shows an increase in activation as the shelf-life of the platelet concentrated component increases (Dewland, 2017).…”

Section: Resultsmentioning

confidence: 99%

Platelet Morphological Assessment Based on Shelf-Life of Concentrated Platelet Components

Astuti¹,

Maharani²,

Susadma³

2021

biomedika

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Concentrated platelet components are the components or platelets that are processed through centrifugation from whole blood or a single donor. The components of the concentrate platelets are stored in an agitator at a temperature of 20-24◦C with a maximum shelf-life of five days. During the storage, a change in component metabolism occurs, which is characterized by alteration in platelet morphology as an indicator of decreasing quality of platelet components. The purpose of this study was to determine the quality of the components of platelets based on the shelf-life by measuring the platelet morphological values with the Kunicki method. This study applied an experimental method by conducting inspections of the regional blood transfusion unit (UTDD) of the Indonesian Red Cross of Jakarta Capital Special Region (PMI DKI Jakarta). Platelet morphological values of six components of platelets were examined by the Kunicki method on day 0 to day 5 of shelf-life. Quality assessment was carried out based on a significant difference-dependent t-test and comparison with normal value. The results of the study by performing statistical analysis of the significant difference-dependent t-test showed a significant difference of values between day 0 and day 1 to day 5 of shelf-life. Comparison with normal values depicted that all samples stored on day 4 had a morphological value of more than 200. On day 5 of shelf-life, the platelet morphology values of five samples were below 200. The quality of the concentrated platelet components is said “good” if it is above 200. This study concluded that there was a significant difference in the platelet morphology values between the components of concentrated platelets on day 0 and day 1 to day 5 of shelf-life. All samples were of good quality until day 4 of storage. However, on day 5 of storage, only one sample was good in quality.

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Section: Resultsmentioning

confidence: 99%

Platelet Morphological Assessment Based on Shelf-Life of Concentrated Platelet Components

Astuti¹,

Maharani²,

Susadma³

2021

biomedika

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“…3,13 Whereas one case of severe HDFN, which required exchange transfusion, has been attributed to anti-SC4. 16 In 2018 anti-SC2 was reported to cause acute hemolytic transfusion reaction. 3 Autoantibodies against SC1 and SC3 have been associated with autoimmune hemolytic anemia.…”

Section: Discussionmentioning

confidence: 99%

“…14 SC8 named SCAR has been recently recognized by Srivastava et al in a Saudi Arabian patient. 13,15 The gene encoding the Scianna system is positioned on chromosome number one 16 and its antigens are braced on ERAMP; a red cell membranerelated protein. 14,15 These are well established in neonates and are expressed on cord RBCs.…”

mentioning

confidence: 99%

“…3 Antibodies directed against Scianna antigens are infrequent and there is slight knowledge concerning their clinical importance. 16 Scianna antibodies are typically created due to RBC sensitization by pregnancy or transfusion, although there are some examples of naturally occurring anti-SC2 antibodies. These are generally IgG type, react efficiently in the antiglobulin phase and do not bind complement.…”

mentioning

confidence: 99%

“…These are generally IgG type, react efficiently in the antiglobulin phase and do not bind complement. 16 Molecular testing is now being extensively used for genotyping red cell antigens and is considered an indispensable tool in the field of immune hematology. 18 Genotyping has made it possible to identify a far larger number of erythrocyte antigens than by serological techniques.…”

mentioning

confidence: 99%

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Genotyping for Scianna blood group system polymorphism in Pakistan.

Jadoon

Muhammad

Aslam

et al. 2023

TPMJ

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Objective: To establish the occurrence of Scianna blood group alleles via genotyping in Pakistani blood donors. Study Design: Cross-sectional Analytical study. Setting: Department of Hematology, Army Medical College (AMC) in association with the Armed Forces Institute of Transfusion (AFIT) Rawalpindi. Period: January 2019 and January 2020. Material & Methods: In this study 300 blood donors were incorporated after taking informed consent; DNA was extracted from their EDTA anticoagulated blood samples and tested for SC*01 and SC*02 alleles by Sequence-Specific Primer Polymerase Chain Reaction (PCR-SSP), which was ensued by gel electrophoresis. The allele frequencies were established and Hardy Weinberg equilibrium was determined. Chi-square test was applied to compare the observed and expected genotype frequencies as well as for comparison with published allele frequencies of other populations. We used SPSS version 22 for the analysis of study data. Results: All donors were homozygous for SC*01, demonstrating allele frequency for SC*01 nearly 1.00. Genotype frequencies were congruent with the Hardy Weinberg equilibrium. The difference between the allele frequencies of SC*01 and SC*02 alleles of population of Pakistan was not statistically significant when compared to those of other populations. Conclusion: This maiden research illustrates Scianna blood group distribution in Pakistani community by PCR-SSP. This method is cheap and proficient and can be applied in developing nations. The findings of this study can aid in the production of local red cell panels, detection of uncommon blood types, and setting up of a domestic rare blood donor platform.

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The impact of an antibody investigation algorithm emphasizing specificity on reducing potential false‐positive warm autoantibody detection at a Canadian tertiary care centre

Hutspardol,

Boyd,

Zamar

et al. 2023

Vox Sanguinis

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Background and ObjectivesTo reduce potential false‐positive warm autoantibody (WAA) by solid‐phase red cell adherence assay (SPRCA), our centre implemented a new antibody investigation algorithm (AIA) by classifying cases with panreactive SPRCA but negative saline‐indirect antiglobulin test as ‘antibody of undetermined significance’ (AUS) after excluding clinically significant antibodies. We assessed the effects of the new AIA and subsequent alloantibody formation in patients with AUS.Materials and MethodsSamples from patients with positive SPRCA screens between 1 September 2017 and 31 August 2021 were selected for the study. Frequencies of antibodies classified by the old and new AIAs were compared using Fisher's exact test. Patient demographics, transfusion history and antibody formation in cases of AUS were collected.ResultsA significant reduction in potential WAA frequencies from 127/1167 (11%) to 53/854 (6%) was observed (p < 0.001) when compared between the old and new AIAs among 2021 positive SPRCA antibody screens. While no patients with AUS later transitioned to potential WAA using the new AIA, four patients developed alloantibodies, including anti‐E, anti‐C, both anti‐C and anti‐E, and anti‐Wra.ConclusionA significant reduction in the frequencies of potential false‐positive WAA detection at our centre was observed after implementing the new AIA, leading to less resource and phenotypically matched red blood cell (RBC) use. Some patients still developed subsequent RBC alloimmunization, so clinically relevant alloantibodies should be carefully excluded before determining AUS, taking forming or evanescent antibodies into consideration.

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Modern Blood Banking and Transfusion Practices

Cited by 14 publications

References 0 publications

Platelet Morphological Assessment Based on Shelf-Life of Concentrated Platelet Components

Platelet Morphological Assessment Based on Shelf-Life of Concentrated Platelet Components

Genotyping for Scianna blood group system polymorphism in Pakistan.

The impact of an antibody investigation algorithm emphasizing specificity on reducing potential false‐positive warm autoantibody detection at a Canadian tertiary care centre

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