Modern Solution Culture Techniques

Asher, C. J.; Edwards, D. G.

doi:10.1007/978-3-642-68885-0_4

Cited by 81 publications

(38 citation statements)

References 67 publications

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“…Based on studies in flowing nutrient solutions with constant external concentrations, between 0.25 and 3.6 ~ Mn2+ is considered adequate for the growth of most plant species (2). In a more recent report (1) these required concentrations (denoted as 'rhizosphere Mn concentrations') appear to vary between 0.01 and 50.0 ~ Mn, reflecting differences between plant species.…”

Section: Concentrations Of Mn Requiredfor Adequate Growthmentioning

confidence: 98%

Mechanisms of Manganese Acquisition by Roots from Soils

Marschner¹

1988

Manganese in Soils and Plants

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Section: Concentrations Of Mn Requiredfor Adequate Growthmentioning

confidence: 98%

Mechanisms of Manganese Acquisition by Roots from Soils

Marschner¹

1988

Manganese in Soils and Plants

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“…This is comparable to recent reported yields of Gnome soybeans grown under field conditions (0.39 kg m-2, 16.4 g per 100 seeds, Smiciklas et a1 1992). Historically, when plant scientists have used hydroponic culture techniques, nutrient solutions have been replaced frequently to prevent deficiencies of individual ions (Imsande and Ralston 1981;Asher and Edwards 1983). The expense of the nitrogen isotope in the present study dictated a recirculation of the nutrient solution.…”

Section: Nutrient Solution Recommendationsmentioning

confidence: 99%

Uniformly ¹⁵N‐labeled soybean seeds produced for use in human and animal nutrition studies: Description of a recirculating hydroponic growth system and whole plant nutrient and environmental requirements

Grusak

Pezeshgi

1994

J Sci Food Agric

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A recirculating hydroponic technique which enables the uniform "Nlabeling of soybean plants has been developed. Daily net influx of nitrate, potassium, calcium, chloride and phosphate, as well as total solution uptake, was measured for a crop of 60 plants grown in a 3 m2 area. A protocol is presented which (i) maintains adequate nutrient concentrations for the crop, (ii) adds nutrients and water as required with no exchange of solution, and (iii) minimizes the addition and use of "N. With this technique, 6.5 mol of 15N were used to generate 1 kg (DW) of soybean seeds (350 g protein kg-'; 98 atm% 15N) and 3.1 kg (DW) total biomass; recovery of nitrogen in the total biomass was 95%. "Nlabeled soy protein isolate or harvested plant parts (eg leaves or stems) can be used to investigate nutrient absorption and metabolism in human or animal studies.

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“…There are two possibilities that could account for this observed change in Mn accumulation rate. Firstly, it is possible that in barley, the minimum nutrient concentration (Cmin) from which plants can accumulate a particular nutrient (see reviews by Asher, 1981;Asher and Edwards, 1983) such as Mn, changed with developmental age of plants; or secondly, these plants were affected by the absence of Mn for the first week of growth and the subsequent accumulation rate responded to some homeostatic mechanism which increased this rate by a manner similar in concept to those already shown for P and Fe R6mheld and Marschner, 1986). The latter seems unlikely as a Mn-specific response to the absence of Mn, because the effect of plant age on accumulation rate was common to other nutrient cations.…”

Section: Accumulation Rate Of Mnmentioning

confidence: 99%

Using a chelate-buffered nutrient solution to establish the critical solution activity of Mn2+ required by barley (Hordeum vulgare L.)

et al. 1993

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Relatively little is known about the responses of plants to micronutrients when these nutrients are maintained at the very low levels found in soils of low fertility. We have determined the requirement of barley (Hordeum vulgare L. cv Herta) for ionic Mn 2+ in plant culture solutions using the chelating agent HEDTA as a buffer for micronutrient metal ions. The chemical activity of Mn 2+ was varied approximately 10,000-fold from log(Mn 2÷) = -10.8 to -6.8 (pMn 10.8 to pMn 6.8), while holding constant the activities of the other micronutrient cations. Growth, appearance, and composition of 'Herta' barley indicated that log(Mn 2÷) of approximately -8.3 would permit optimal dry matter production and normal plant development. The specific accumulation rate of Mn by 15 to 23 day old seedlings was a linear function of the Mn 2÷ activity in solution. At log(Mn 2÷) of about -9.8 or below, barley seedlings were unable to accumulate significant amounts of Mn, and at some harvests, suffered a net loss of Mn to solution. Seedlings younger than 11 days old were ineffective accumulators of several cations, including Mn, Fe, Zn, Cu, Mg, and Ca. Differences in Mn availability did not influence uptake of other cations, except that Cu uptake by roots increased with increasing Mn uptake.

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Modern Solution Culture Techniques

Cited by 81 publications

References 67 publications

Mechanisms of Manganese Acquisition by Roots from Soils

Mechanisms of Manganese Acquisition by Roots from Soils

Uniformly ¹⁵N‐labeled soybean seeds produced for use in human and animal nutrition studies: Description of a recirculating hydroponic growth system and whole plant nutrient and environmental requirements

Using a chelate-buffered nutrient solution to establish the critical solution activity of Mn2+ required by barley (Hordeum vulgare L.)

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Modern Solution Culture Techniques

Cited by 81 publications

References 67 publications

Mechanisms of Manganese Acquisition by Roots from Soils

Mechanisms of Manganese Acquisition by Roots from Soils

Uniformly 15N‐labeled soybean seeds produced for use in human and animal nutrition studies: Description of a recirculating hydroponic growth system and whole plant nutrient and environmental requirements

Using a chelate-buffered nutrient solution to establish the critical solution activity of Mn2+ required by barley (Hordeum vulgare L.)

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Uniformly ¹⁵N‐labeled soybean seeds produced for use in human and animal nutrition studies: Description of a recirculating hydroponic growth system and whole plant nutrient and environmental requirements