Modern surface plasmon resonance for bioanalytics and biophysics

Couture, Maxime; Zhao, Sandy Shuo; Masson, Jean-François

doi:10.1039/c3cp50281c

Cited by 160 publications

(140 citation statements)

References 234 publications

(282 reference statements)

Supporting

Mentioning

140

Contrasting

Order By: Relevance

“…Possible bimolecular interaction studies include affinity, kinetics, thermodynamics, and specificity and concentration analysis of interacting molecules. Currently this technology is widely applied in bioanalytical areas such as medical diagnostics, bioanalysis, biopharmaceutics, drug discovery and proteomics [21][22][23]. SPR offers several advantages over alternative immunoassay techniques like ELISA or radio immunoassay (RIA).…”

Section: Surface Plasmon Resonance (Spr) Assaysmentioning

confidence: 99%

Data quality in drug discovery: the role of analytical performance in ligand binding assays

Wätzig

Oltmann-Norden

Steinicke

et al. 2015

J Comput Aided Mol Des

View full text Add to dashboard Cite

Despite its importance and all the considerable efforts made, the progress in drug discovery is limited. One main reason for this is the partly questionable data quality. Models relating biological activity and structures and in silico predictions rely on precisely and accurately measured binding data. However, these data vary so strongly, such that only variations by orders of magnitude are considered as unreliable. This can certainly be improved considering the high analytical performance in pharmaceutical quality control. Thus the principles, properties and performances of biochemical and cell-based assays are revisited and evaluated. In the part of biochemical assays immunoassays, fluorescence assays, surface plasmon resonance, isothermal calorimetry, nuclear magnetic resonance and affinity capillary electrophoresis are discussed in details, in addition radiation-based ligand binding assays, mass spectrometry, atomic force microscopy and microscale thermophoresis are briefly evaluated. In addition, general sources of error, such as solvent, dilution, sample pretreatment and the quality of reagents and reference materials are discussed. Biochemical assays can be optimized to provide good accuracy and precision (e.g. percental relative standard deviation <10 %). Cell-based assays are often considered superior related to the biological significance, however, typically they cannot still be considered as really quantitative, in particular when results are compared over longer periods of time or between laboratories. A very careful choice of assays is therefore recommended. Strategies to further optimize assays are outlined, considering the evaluation and the decrease of the relevant error sources. Analytical performance and data quality are still advancing and will further advance the progress in drug development.

show abstract

Section: Surface Plasmon Resonance (Spr) Assaysmentioning

confidence: 99%

Data quality in drug discovery: the role of analytical performance in ligand binding assays

Wätzig

Oltmann-Norden

Steinicke

et al. 2015

J Comput Aided Mol Des

View full text Add to dashboard Cite

show abstract

“…Plasmonic biosensors are heralded due to high sensitivity, low limit of detection and label-free detection. A series of applications were developed in biomedical sciences, environmental detection and industrial applications based on these advantages [1]. However, plasmonic biosensors remain largely susceptible to nonspecific adsorption, limiting their applications in biofluids [1,2].…”

Section: Introductionmentioning

confidence: 99%

SPR-MS: from identifying adsorbed molecules to image tissues

et al. 2015

View full text Add to dashboard Cite

Surface plasmon resonance (SPR) sensors have become valuable analytical sensors for biomolecule detection. While SPR is heralded with high sensitivity, label-free and real-time detection, nonspecific adsorption and detection of ultralow concentrations remain issues. Nonspecific adsorption can be minimized using adequate surface chemistry. For example, we have employed peptide monolayers to reduce nonspecific adsorption of crude serum or cell lysate. It is important to uncover the nature of molecules nonspecifically adsorbing to surfaces in these biofluids, to further improve understanding of the nonspecific adsorption processes. Mass spectrometry (MS) provides a complementary tool to SPR to identify biomolecule adsorbed to surface. Trypsic digestion of the proteins adsorbed to surfaces led to identification of characteristic peptides from the proteins involved in nonspecific adsorption. Nonspecific adsorption in crude cell lysate results mainly from lipids, as confirmed with SPR and MS but proteins were observed on some surfaces. In another application of SPR and MS, imaging SPR can be used in combination to imaging MS to image tissue sections. Thin sections of mouse liver were inserted in the fluidic chamber of a SPRi instrument and proteins were transferred to the SPRi chip. The SPR chip was then imaged using MALDI imaging MS to identify the biomolecules that were transferred to the SPRi chip.

show abstract

“…In particular, if the surface plasmon of SPR is confined in nanostructures with size comparable to or smaller than the wavelength of light, a higher sensitivity to small molecules can be achieved [37,39]. In this context, LSPR, based on tracking the local refractive index changes due to biomolecular reaction, represents a promising technique.…”

Section: Engineered Substrates For Enhanced Sensitivitymentioning

confidence: 99%

Emerging applications of label-free optical biosensors

et al. 2017

View full text Add to dashboard Cite

Abstract:Innovative technical solutions to realize optical biosensors with improved performance are continuously proposed. Progress in material fabrication enables developing novel substrates with enhanced optical responses. At the same time, the increased spectrum of available biomolecular tools, ranging from highly specific receptors to engineered bioconjugated polymers, facilitates the preparation of sensing surfaces with controlled functionality. What remains often unclear is to which extent this continuous innovation provides effective breakthroughs for specific applications. In this review, we address this challenging question for the class of label-free optical biosensors, which can provide a direct signal upon molecular binding without using secondary probes. Label-free biosensors have become a consolidated approach for the characterization and screening of molecular interactions in research laboratories. However, in the last decade, several examples of other applications with high potential impact have been proposed. We review the recent advances in label-free optical biosensing technology by focusing on the potential competitive advantage provided in selected emerging applications, grouped on the basis of the target type. In particular, direct and real-time detection allows the development of simpler, compact, and rapid analytical methods for different kinds of targets, from proteins to DNA and viruses. The lack of secondary interactions facilitates the binding of small-molecule targets and minimizes the perturbation in single-molecule detection. Moreover, the intrinsic versatility of label-free sensing makes it an ideal platform to be integrated with biomolecular machinery with innovative functionality, as in case of the molecular tools provided by DNA nanotechnology.

show abstract

Modern surface plasmon resonance for bioanalytics and biophysics

Cited by 160 publications

References 234 publications

Data quality in drug discovery: the role of analytical performance in ligand binding assays

Data quality in drug discovery: the role of analytical performance in ligand binding assays

SPR-MS: from identifying adsorbed molecules to image tissues

Emerging applications of label-free optical biosensors

Contact Info

Product

Resources

About