Moderne Siebböden — Maximale Dampfgeschwindigkeit

Boronyák, I.; Gyökhegyi, L.

doi:10.1002/1522-2640(200003)72:3<224::aid-cite224>3.0.co;2-2

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…Over the past decade, the potential of BMSCs to provide regenerative treatment strategies in cardiac disease has been investigated. 16,18,[23][24][25] Studies have found that BMSCs differentiate in vitro to cardiomyocytes 28,30 and SMCs 26,31-33 ; however, there has not yet been clear evidence of BMSC differentiation to endothelial lineages when seeded on fibrous scaffolds, under biomechanical environments. ESCs, on the other hand, possess the ability to differentiate to cardiac phenotypes, including both SMCs and ECs.…”

Section: Discussionmentioning

confidence: 99%

“…6,[23][24][25][26][27][28] This bioreactor is capable of replicating physiological hemodynamic conditions during in vitro tissue development, which may play an essential role in engineered heart valve tissue formation. 24,25 After culturing of the scaffolds (n = 6) statically for a total of 8 days, they were separated into two groups: static and flow. Scaffolds (n = 3/group) were then cultured for a 2-week period in static and dynamic conditions (22 days of total culture time).…”

Section: Tissue Engineering Experimentsmentioning

confidence: 99%

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Periodontal Ligament Cells Cultured Under Steady-Flow Environments Demonstrate Potential for Use in Heart Valve Tissue Engineering

Martinez

Rath

Gulden

et al. 2013

Tissue Engineering Part A

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A major drawback of mechanical and prosthetic heart valves is their inability to permit somatic growth. By contrast, tissue-engineered pulmonary valves potentially have the capacity to remodel and integrate with the patient. For this purpose, adult stem cells may be suitable. Previously, human periodontal ligament cells (PDLs) have been explored as a reliable and robust progenitor cell source for cardiac muscle regeneration (Pelaez, D. Electronic Thesis and Dissertation Database, Coral Gables, FL, May 2011). Here, we investigate the potential of PDLs to support the valve lineage, specifically the concomitant differentiation to both endothelial cell (EC) and smooth muscle cell (SMC) types. We were able to successfully promote PDL differentiation to both SMC and EC phenotypes through a combination of stimulatory approaches using biochemical and mechanical flow conditioning (steady shear stress of 1 dyne/cm(2)), with flow-based mechanical conditioning having a predominant effect on PDL differentiation, particularly to ECs; in addition, strong expression of the marker FZD2 and an absence of the marker MLC1F point toward a unique manifestation of smooth muscle by PDLs after undergoing steady-flow mechanical conditioning alone, possible by only the heart valve and pericardium phenotypes. It was also determined that steady flow (which was performed using a physiologically relevant [for heart valves] magnitude of ~5-6 dynes/cm(2)) augmented the synthesis of the extracellular matrix collagen proteins. We conclude that under steady-flow dynamic culture environments, human PDLs can differentiate to heterogeneous cell populations that are relevant to heart valve tissue engineering. Further exploration of human PDLs for this purpose is thus warranted.

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