Moringa oleifera Lam is a plant found in Brazil, with applications in the food, agricultural, industrial, and medicinal areas. M. oleifera has favourable healing properties, and chitosan has healing potential. This polymer is a biocompatible, non-toxic and biodegradable polycationic biopolymer. Chitosan membranes have good mechanical strength and selective molecular permeability. These membranes used in food packaging, artificial skin, wound healing, drug delivery systems and other applications. This study aims to prepare chitosan membranes to incorporate ethanol extract from the seed of M. oleifera for use as a biomaterial. We first describe methods to obtain M. oleifera extract, and second highlight method to prepare membranes. We use Fourier Transform Infrared (FTIR) Spectroscopy to verify the incorporation of extract in the membrane. The results obtained was homogeneous and malleable membranes. The absorption spectrum in the infrared region showed bands characteristic of chemical groups of both chitosan and the incorporated extract. This incorporation can be applied to improve the performance of membranes with chitosan for use as biomaterial, such as wound healing.