Modification and Characterization of Lactoferrin-Iron Free with Methylimidazolium N-ethylamine Ionic Liquid as Potential Drugs Anti SARS-CoV-2

Abstract

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Small Molecules of Lactoferrin Modification by Ionic Liquids Against SARS-CoV-2: Molecular Docking Enhanced the Results

“…The high proportion of glycosylation verifies the methodology of MIE-NH2 following reductive amination, it was used for labelling of Nglycans [17]. According to our previous studies, ionic liquids drive lactoferrin iron free (IL-Lf-iron free) [18]. There was found proximity 42 types of N-glycans with diverse potential sites Nglycosylation in bovine Lactoferin (BLf), the different N-glycans with all structures sites [29,30].…”

“…This work demonstrated the catalytic mechanism of the derivatization of Lactoferrin-N-glycans with ionic liquid MIE-NH2 following the reductive amination. The free aldehyde realized in the acidic medium and by reducing ligand as sodium cyanoborohydride, which possesses significant converted the carbonyl to an imine by the NH2 group of MIE-NH2 (Scheme 1 , b) [18]. Recent scientific research focused on the progress in protein-based nanomedicine, albuminpaclitaxel as nanoparticles have been introduced in novel therapeutics and used for the treatment of cancer and viral infections [10,29].…”

“…Tert-butyl N-(2-bromoethyl) carbamate (225 mg, 1.1 mmol) was reacted with Nmethylimidazole (82 mg, 1 mmol) in an anhydrous mixture solvent of CH3CN and t-BuOH [5 mL (3/2, v/v)], reaction mixture refluxing at 80 ºC for 2 days. Removing unreacted materials by washing with ethyl acetate three times and the product was dried under reduced pressure and obtained the light yellow viscous liquid (scheme 1,a) [17][18][19] The viscous liquid (1) IL-Br-1 (289 mg g, 85.7 mmol) was generated by stirring with KBF4 (1.1 equiv.) in water solution for 24 h at room temperature.…”

“…Derivatization solution contains 70 mM MIE-NH2 and 0.1 M sodium cyanoborohydride in dimethyl sulfoxide/acetic acid solution (7:3,v/v)) was added to a sample of Lactoferrin until completely dissolved. The derivatization mixture was mixed by ultrasonic for about 30 minutes and incubated at 70 -90 °C for 4 h [17,18].…”

“…Exploring and identifying the new characterization of novel ionic liquid delivers reacting with oligosaccharides of glycoproteins. Several interesting studies are encouraging for discovers the new application of ionic liquids as potential drugs antimicrobial including anti-coronavirus disease 2019 [10]. Studying the activity of glycoproteins is the assessment of the contributions of individual glycans to the observed bioactivities.…”