Modification and characterization of natural aluminosilicates, expanded perlite, and its application to immobilise α – amylase from A. oryzae

Rodríguez, Jorge A.; Soria, Francisco Fernando; Geronazzo, Hugo Isidoro; Destéfanis, Hugo Alberto

doi:10.1016/j.molcatb.2017.01.012

Cited by 14 publications

(9 citation statements)

References 16 publications

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“…α-Amylase (1,4-D-glucan glucanohydrolase) catalyses the hydrolysis of the α-D-(1,4) glycosidic linkages of glycogen, starch, and other oligosaccharides, and is found in microorganisms, mammalian tissues and plants 3,4 . These enzymes play important roles in industry and are used many fields, including paper, textiles, food and baking, and others 5,6 . However, the industrial application of amylases is confined by their short lifetime sensitivity to environmental factors, poor operational storage stability and high production costs which leads to difficulties in their reuse and recovery 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability

Al-Najada

Almulaiky

Aldhahri

et al. 2019

Sci Rep

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In this study, amidrazone acrylic fabric was applied as an immobilising support for α-amylase. The immobilised α-amylase was characterised by Fourier transform infrared spectroscopy and scanning electron microscopy. Furthermore, the optimum conditions for immobilisation efficiency, immobilisation time, reusability, kinetic parameters and pH, for the immobilisation process were examined. The study demonstrated that with 4% cyanuric chloride, and a pH of 7.0, the highest immobilization efficiency of 81% was obtained. Around 65% of the initial activity was maintained after storage at 4 °C for 8 weeks. The immobilised enzyme retained 53% of its original activity after being reused 15 times and exhibited improved stability compared with the free enzyme in relation to heavy metal ions, pH, temperature and inhibitors. The immobilised enzyme presented kinetic parameters of 2.6 mg starch and 0.65 µmol maltose/mL for K m and V max respectively, compared with 3.7 mg starch and 0.83 µmol maltose/ mL for the free enzyme. The improvements in the enzyme’s catalytic properties, stability and reusability obtained from immobilisation make amidrazone acrylic fabric support a good promising candidate for bio-industrial applications.

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Section: Introductionmentioning

confidence: 99%

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability

Al-Najada

Almulaiky

Aldhahri

et al. 2019

Sci Rep

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“…Perlites, either as native materials or expanded, due to their properties (low density, high porosity, chemical inertness and non-toxicity), are versatile materials and used for the preparation of catalysts, ceiling tiles, pipe insulation, gypsum wallboard, cryogenic insulation, fillers, materials for filtering (filters), aggregates for light cement, removal of heavy metals and adsorption of particulates from the atmosphere, thermal insulators, absorption of oil, roof panels, foams, fire retardants, brick products, horticulture, production of container glass, in the ceramic industry (as a component in the ceramic mass), microorganism carriers, materials for improving the mechanical and thermal properties of cement mortar, and glaze products (AHMADIA et al, 2018;SODE-YAMA et al, 1999;ZAFIROVSKI et al, 1987;VIJAYARAGHAVAN and RAJA, 2014;RODRIGUEZ et al, 2016). The application of perlite for these purposes depends on the physico-chemical characteristics, as well as from its mineralogical composition.…”

Section: Introductionmentioning

confidence: 99%

Chemical, mineralogical and structural features of native and expanded perlite from Macedonia

Reka

Pavlovski²,

Lisichkov

et al. 2019

Geol Cro

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The physico-mechanical, chemical and mineralogical characteristics of volcanic glass (perlite) from the Mariovo region (Macedonia) as well as the mineralogical changes that occur during its thermal treatment were investigated to demonstrate its utilization for industrial use. The native perlite was characterized by chemical analysis, X-ray powder diffraction (XRPD), infrared (IR) spectroscopy, thermal analysis (TGA/DTA), scanning electron microscopy (SEM-EDX), transmission electron microscopy (TEM), and solid-state NMR. The chemical examination suggests that the perlite represents an acidic volcanic rock with a high percentage of SiO 2 (72.45%), high in alkali metal oxides (4.21 wt.% K 2 O, 3.56 wt.% Na 2 O), with a loss of ignition 3.54 wt.%. Results from the XRPD indicated major amorphous behaviour, with low amounts of feldspars, quartz, and cristobalite. SEM examinations revealed glassy structure with presence of certain pores (dimensions ranging from 50-100 μm). The determined expansion coefficient was 20 times its original volume. XRPD of expanded perlite compared to the native perlite depicted new intensive peaks of cristobalite. SEM and TEM revealed irregular morphology with broken or ragged edges. On the basis of the chemical and mineralogical composition, the studied perlite is classified as an appropriate material suitable as ceramic flux to lower the sintering temperature.

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“…The expansion that shows the structure of the perlite after a thermal treatment about 850°C (Figures 3 and 4) allows to host large molecules such as proteins and enzymes, which has great potential in the field of enzymatic catalysts and biosensors as well as the diagnosis of diseases. Thus, Rodríguez et al have immobilized α-amylase onto expanded perlite [57], while Demirbas et al have immobilized casein [58]. Pezzella et al have adsorbed laccase on expanded perlite to adsorb dyes [59].…”

Section: Adsorption Of Biomoleculesmentioning

confidence: 99%

Volcanic Glass and its Uses as Adsorbent

Cecilia¹,

Autie-Pérez²,

ManuelLabadie-Suarez³

et al. 2018

Volcanoes - Geological and Geophysical Setting, Theoretical Aspects and Numerical Modeling, Applications to Industry and Their

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Volcanic glasses are an amorphous phyllosilicates formed by the fast cooling of the magma. The physicochemical properties of volcanic glasses are directly related to their chemical composition. Thus, the rhyolitic magma, which presents the highest SiO 2 percentage, displays a high viscosity, which leads to explosive eruptions by the ex-solution of H 2 O, CO 2 , and SO 2 , when the pressure diminishes generates a macroporous structure with interesting applications in construction, as abrasive, acoustic, filter as well as in the agriculture field. The macroporosity of volcanic glass allows to host large molecules as biomolecules, tensoactives, or dyes. On the other hand, the existence of hydroxyl groups in this amorphous aluminosilicate also favors the adsorption of cations and anions, so the volcanic glass is an economical adsorbent to retain heavy metals or radioactive cations.

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Modification and characterization of natural aluminosilicates, expanded perlite, and its application to immobilise α – amylase from A. oryzae

Cited by 14 publications

References 16 publications

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability

Chemical, mineralogical and structural features of native and expanded perlite from Macedonia

Volcanic Glass and its Uses as Adsorbent

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