Modification of Antioxidative and Antiapoptotic Genes Expression in irradiated K562 Cells Upon Fullerenol C&lt;SUB&gt;60&lt;/SUB&gt;(OH)&lt;SUB&gt;24&lt;/SUB&gt; Nanoparticle Treatment

Stankov, Karmen; Borišev, Ivana; Kojić, Vesna; Rutonjski, Lazar; Bogdanovic, Gordana; Djordjević, Aleksandar

doi:10.1166/jnn.2013.6847

Cited by 19 publications

(10 citation statements)

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“…[14][15][16][17] Polyhydroxylated fullerenes, also known as fullerols [C 60 (OH) n ], belong to a group of antioxidant nanoparticles that exhibit strong antioxidant activity in both in vitro and in vivo biological systems. 13 It has been demonstrated that the anti-inflammatory, 18 antiapoptotic, 16,17 radioprotective, 19,20 and neuroprotective effects 21,22 and the enzyme-inhibitory activity of fullerols 13 have been attributed to their ability to scavenge free radicals, such as superoxide anion radical, hydroxy radical, and nitrous oxide radical. It has since been well established that induction of Nrf2-regulated phase II antioxidant enzymes by nutritional or pharmacological intervention is an effective approach to combat the toxicities of ROS and to protect cells against oxidative stress.…”

Section: Ye Et Almentioning

confidence: 99%

“…In an animal irradiated model that is associated with oxidative stress, pretreatment with C 60 (OH) 24 showed radioprotective effects by scavenging ROS and increasing the antioxidant enzyme activities. 19,20 It has also been reported that C 60 (OH) 24 shows hepatoprotective effects in doxorubicin-treated rats by acting as an antioxidant. 44,45 Hence, it is possible to propose that C 60 (OH) 24 pretreatment prevented deleterious effects of ROS by direct ROS scavenging or increasing the antioxidant enzyme activities.…”

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confidence: 96%

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Polyhydroxylated fullerene attenuates oxidative stress-induced apoptosis via a fortifying Nrf2-regulated cellular antioxidant defence system

Ye¹,

Chen²,

Jiang³

et al. 2014

IJN

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Polyhydroxylated derivatives of fullerene C 60 , named fullerenols (C 60 [OH] n ), have stimulated great interest because of their potent antioxidant properties in various chemical and biological systems, which enable them to be used as a new promising pharmaceutical for the future treatment of oxidative stress-related diseases, but the details remain unknown. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a principal transcription factor that regulates expression of several antioxidant genes via binding to the antioxidant response element and plays a crucial role in cellular defence against oxidative stress. In this study we investigated whether activation of the Nrf2/antioxidant response element pathway contributes to the cytoprotective effects of C 60 (OH) 24 . Our results showed that C 60 (OH) 24 enhanced nuclear translocation of Nrf2 and upregulated expression of phase II antioxidant enzymes, including heme oxygenase-1 (HO-1), NAD(P)H: quinine oxidoreductase 1, and γ-glutamate cysteine ligase in A549 cells. Treatment with C 60 (OH) 24 resulted in phosphorylation of p38 mitogen-activated protein kinases (p38 MAPK), extracellular signal-regulated kinases, and c-Jun-N-terminal kinases. By using inhibitors of cellular kinases, we showed that pretreatment of A549 cells with SB203580, a specific inhibitor of p38 MAPK, abolished nuclear translocation of Nrf2 and induction of HO-1 protein induced by C 60 (OH) 24 , indicating an involvement of p38 MAPK in Nrf2/HO-1 activation by C 60 (OH) 24 . Furthermore, pretreatment with C 60 (OH) 24 attenuated hydrogen peroxide-induced apoptotic cell death in A549 cells, and knockdown of Nrf2 by small interfering ribonucleic acid diminished C 60 (OH) 24 -mediated cytoprotection. Taken together, these findings demonstrate that C 60 (OH) 24 may attenuate oxidative stress-induced apoptosis via augmentation of Nrf2-regulated cellular antioxidant capacity, thus providing insights into the mechanisms of the antioxidant properties of C 60 (OH) 24 .

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Section: Ye Et Almentioning

confidence: 99%

mentioning

confidence: 96%

Polyhydroxylated fullerene attenuates oxidative stress-induced apoptosis via a fortifying Nrf2-regulated cellular antioxidant defence system

Ye¹,

Chen²,

Jiang³

et al. 2014

IJN

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“…But the expression of anti-apoptotic and cytoprotective genes was modulated. Similarly, most of the cytoprotective genes were elevated with the pre-treated C60(OH)24, such as CAT, Mn-SOD, nitric oxide synthase, glutathione Stransferase isoform GSTA4, glutathione peroxidase and gamma-glutamyltransferase [40].…”

Section: Radical Scavenger: Protect Cells From the Radiationmentioning

confidence: 95%

Biological applications of hydrophilic C60 derivatives (hC60s)− a structural perspective

Zhu

Sollogoub

Zhang

2016

European Journal of Medicinal Chemistry

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Abstract:Reactive oxygen species (ROS) generation and radical scavenging are dual properties of hydrophilic C60 derivatives (hC60s). hC60s eliminate radicals in dark, while they produce reactive oxygen species (ROS) in the presence of irradiation and oxygen. Compared to the pristine C60 suspension, the aqueous solution of hC60s is easier to handle in vivo. hC60s are diverse and could be placed into two general categories: covalently modified C60 derivatives and pristine C60 solubilized non-covalently by macromolecules. In order to present in detail, the above categories are broken down into 8 parts: C60(OH)n, C60 with carboxylic acid, C60 with quaternary ammonium salts, C60 with peptide, C60 containing sugar, C60 modified covalently or non-covalently solubilized by cyclodextrins (CDs), pristine C60 delivered by liposomes, functionalized C60-polymer and pristine C60 solubilized by polymer. Each hC60 shows the propensity to be ROS producer or radical scavenger. This preference is dependent on hC60s structures. For example, major application of C60(OH)n is radical scavenger, while pristine C60/γ-CD complex usually serves as ROS producer. In addition, the electron acceptability and innate hydrophobic surface confer hC60s with O2 uptake inhibition, HIV inhibition and membrane permeability. In this review, we summarize the preparation methods and biological applications of hC60s according to the structures.

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“…Recent research, investigating the role of fullerenol C 60 (OH) 24 in modulation of the response of K562 leukemic cells to irradiation, indicated that fullerenol improved survival of the irradiated cells and produced significant overexpression of antiapoptotic Bcl-2 and Bcl-xL proteins as well as cytoprotective GSTA4, MnSOD, NOS, CAT, and HO-1 genes [39]. …”

Section: Potential Application Of Fullerenols In Radiobiologymentioning

confidence: 99%

Fullerenols as a New Therapeutic Approach in Nanomedicine

Grebowski

Kazmierska

Krokosz

2013

BioMed Research International

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Recently, much attention has been paid to the bioactive properties of water-soluble fullerene derivatives: fullerenols, with emphasis on their pro- and antioxidative properties. Due to their hydrophilic properties and the ability to scavenge free radicals, fullerenols may, in the future, provide a serious alternative to the currently used pharmacological methods in chemotherapy, treatment of neurodegenerative diseases, and radiobiology. Some of the most widely used drugs in chemotherapy are anthracycline antibiotics. Anthracycline therapy, in spite of its effective antitumor activity, induces systemic oxidative stress, which interferes with the effectiveness of the treatment and results in serious side effects. Fullerenols may counteract the harmful effects of anthracyclines by scavenging free radicals and thereby improve the effects of chemotherapy. Additionally, due to the hollow spherical shape, fullerenols may be used as drug carriers. Moreover, because of the existence of the currently ineffective ways for neurodegenerative diseases treatment, alternative compounds, which could prevent the negative effects of oxidative stress in the brain, are still sought. In the search of alternative methods of treatment and diagnosis, today's science is increasingly reaching for tools in the field of nanomedicine, for example, fullerenes and their water-soluble derivatives, which is addressed in the present paper.

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Modification of Antioxidative and Antiapoptotic Genes Expression in irradiated K562 Cells Upon Fullerenol C<SUB>60</SUB>(OH)<SUB>24</SUB> Nanoparticle Treatment

Cited by 19 publications

References 0 publications

Polyhydroxylated fullerene attenuates oxidative stress-induced apoptosis via a fortifying Nrf2-regulated cellular antioxidant defence system

Polyhydroxylated fullerene attenuates oxidative stress-induced apoptosis via a fortifying Nrf2-regulated cellular antioxidant defence system

Biological applications of hydrophilic C60 derivatives (hC60s)− a structural perspective

Fullerenols as a New Therapeutic Approach in Nanomedicine

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