Modification of glassy carbon and gold electrodes with DNA

Pang, Dai‐Wen; Zhang, Min; Wang, Zongli; Qi, Yipeng; Cheng, Jie; Liu, Zi-Ye

doi:10.1016/0022-0728(95)04383-7

Cited by 110 publications

(38 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Peak current values for CVs of the complexes obtained in 5 mM phosphate buffer are signi®cantly higher (by 64 % and 111 % for Cu(phen) 2 2 and Co(phen) 3 3 , respectively) than those measured in 50 mM buffer solution which is in accordance with [7,8,10]. Together with the DE 1y2 change mentioned above it indicates a transition in the interaction mode between the metal species and dsDNA from dominantly electrostatic to dominantly À10 mol cm À2 which represents a saturated monolayer [6].…”

Section: Interactions With the Dna Modi®ed Electrodesupporting

confidence: 88%

See 1 more Smart Citation

Voltammetric Detection of the DNA Interaction with Copper Complex Compounds and Damage to DNA

Labuda¹,

Bučková

Vaníčková

et al. 1999

Electroanalysis

View full text Add to dashboard Cite

Binding reactions of copper(II) complexes of 1,10-phenanthroline (phen) and tetraaza macrocyclic ligand TAAB with calf thymus DNA have been investigated voltammetrically at bare and DNA modi®ed glassy carbon electrodes. The values of binding constant, binding site size, ratio of binding constants for the reduced and oxidized copper complex forms as well as information on the electrostatic and intercalative binding modes were obtained by solution and surface-based methods. The Cu(phen) 2 2 complex mediates the dsDNA cleavage to a higher degree than Cu(TAAB) 2 as indicated by the redox marker Co(phen) 3 3and the anodic signal of the DNA base. A procedure for the damage to DNA detection using dsDNAaGCE biosensor is proposed.

show abstract

Section: Interactions With the Dna Modi®ed Electrodesupporting

confidence: 88%

“…A stable DNA-adsorbed GCE was obtained by evaporation of 5 mL of 5 mg mL À1 dsDNA on the polished electrode surface to dryness similarly to [7]. Compared to the bare GCE, the DNA modi®ed electrode exhibits signi®cantly higher background current according to the thickness of the DNA layer.…”

Section: Interactions With the Dna Modi®ed Electrodementioning

confidence: 99%

Voltammetric Detection of the DNA Interaction with Copper Complex Compounds and Damage to DNA

Labuda¹,

Bučková

Vaníčková

et al. 1999

Electroanalysis

View full text Add to dashboard Cite

show abstract

“…The principal advantages of using surfacebound oligonucleotides over those in solution include ease of purification, conservation of material and reagents, reduction of interference between oligonucleotides and facilitated sample handling (12). Previously explored surfaces for immobilization of DNA include latex beads (5), polystyrene (1), carbon electrodes (22)(23)(24)(25), gold (17,23,(26)(27)(28) and oxidized silicon or glass (3,8,11,(29)(30)(31)(32). The surface chemistries involved with these substrates do not generally possess all of the desired characteristics of an ideal surface.…”

Section: Introductionmentioning

confidence: 99%

Covalent attachment of oligodeoxyribonucleotides to amine-modified Si (001) surfaces

Strother

Hamers

Smith

2000

Nucleic Acids Research

279

308

View full text Add to dashboard Cite

A recently described reaction for the UV-mediated attachment of alkenes to silicon surfaces is utilized as the basis for the preparation of functionalized silicon surfaces. UV light mediates the reaction of tbutyloxycarbonyl (t-BOC) protected ω-unsaturated aminoalkane (10-aminodec-1-ene) with hydrogenterminated silicon (001). Removal of the t-BOC protecting group yields an aminodecane-modified silicon surface. The resultant amino groups can be coupled to thiol-modified oligodeoxyribonucleotides using a heterobifunctional crosslinker, permitting the preparation of DNA arrays. Two methods for controlling the surface density of oligodeoxyribonucleotides were explored: in the first, binary mixtures of 10-aminodec-1-ene and dodecene were utilized in the initial UV-mediated coupling reaction; a linear relationship was found between the mole fraction of aminodecene and the density of DNA hybridization sites. In the second, only a portion of the t-BOC protecting groups was removed from the surface by limiting the time allowed for the deprotection reaction. The oligodeoxyribonucleotide-modified surfaces were extremely stable and performed well in DNA hybridization assays. These surfaces provide an alternative to gold or glass for surface immobilization of oligonucleotides in DNA arrays as well as a route for the coupling of nucleic acid biomolecular recognition elements to semiconductor materials.

show abstract

“…There are many reports about DNA biosensors based on different matrices such as metal(gold/ silver/platinum) surface, graphite (Wang et al 2000), glassy carbon electrode (Cheng et al 2005;Peng et al 1996;Wang and Zhou 2002) and conducting polymers etc (Cha et al 2003;Lassalle et al 2001). DNA immobilized screenprinted carbon electrodes have been proposed for monitoring of toxicants/pollutants, such as bisphenol, o-chlorophenol and 2-aminoanthracene (Lucarelli et al 2002a, b).…”

Section: Introductionmentioning

confidence: 99%

Self-assembled monolayer for toxicant detection using nucleic acid sensor based on surface plasmon resonance technique

Solanki

Prabhakar

Pandey

et al. 2008

Biomed Microdevices

View full text Add to dashboard Cite

Double stranded calf thymus deoxyribonucleic acid (dsCT-DNA) has been covalently immobilized onto self-assembled monolayer (SAM) of beta-merceptoethanol (MCE) on gold substrates via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry for fabrication of the surface plasmon resonance (SPR) based biosensing device. The dsCT-DNA-MCE-SAM/Au bioelectrode has been characterized using electrochemical impedance spectroscopy, cyclic voltammetry, contact angle measurements and atomic force microscopy, respectively. This biosensing device has been utilized for detection of cypermethrin (0.0005 ppm) using electrochemical and SPR techniques, respectively. The mechanism of interaction of cypermethrin with dsCT-DNA immobilized onto MCE-SAM has been proposed.

show abstract

Modification of glassy carbon and gold electrodes with DNA

Cited by 110 publications

References 13 publications

Voltammetric Detection of the DNA Interaction with Copper Complex Compounds and Damage to DNA

Voltammetric Detection of the DNA Interaction with Copper Complex Compounds and Damage to DNA

Covalent attachment of oligodeoxyribonucleotides to amine-modified Si (001) surfaces

Self-assembled monolayer for toxicant detection using nucleic acid sensor based on surface plasmon resonance technique

Contact Info

Product

Resources

About