Modification of HPLC Conditions for the Determinations of Raw Materials, Intermediates and Subsidiary Colors in 5 Kinds of Food Azo Colors.

Abstract

Modification of HPLC conditions for the determinations of raw materials, intermediates and subsidiary colors (organic impurities) in 5 kinds of food azo colors were studied in order to analyze them simply and rapidly. The organic impurities were determined by HPLC using L-column ODS and a gradient system (0.02 mol/L ammonium acetate solution for 10 min, and a gradient with a mixture of acetonitrile and water (7 : 3)). The organic impurities in 163 samples of azo colors subjected to inspection in fiscal year 1999 were determined under the proposed HPLC conditions. It was found that the contents of organic impurities in 163 samples were less than the limit of Japan's Specifications and Standards for Food Additives, 7th Edition.

Lyotropic Chromonic Liquid Crystals and Their Impurities Reveal the Importance of the Position of Functional Groups in Self-Assembly

“…Because commercial SSY products can contain impurities, it is necessary, for the characterization of SSY specimens, to understand quantitatively how and how much impurities affect SSY’s self-assembled structures and phase behavior. Especially, investigating the effects of intrinsic impurities, i.e., raw materials, intermediates, and byproducts of the synthesis, , is essential, but they are uncharted yet. Therefore, we further study the effects of four intrinsic impurities found in our LC–MS analysis, i.e., 2N-SA, SS-AN, RS-SA, and GS-SA, on purified SSY in the next section.…”

Structural Determination of the Subsidiary Colors in Food Blue No. 1 (Brilliant Blue FCF) Aluminum Lake Detected by Paper Chromatography.

“…1 H-NMR and 13 C-NMR spectra were recorded on a Brucker OMEGA600 (Bruker Co., Ltd., Germany) instrument (600 MHz for 1 H, and 150 MHz for 13 C) in methanol-d 4 . The UV-visible (UV-Vis) spectrum was determined with a Hitachi UV-Vis spectrometer U-3210 (Hitachi, Ltd., Tokyo, Japan).…”