Modification of nanocrystalline TiO₂ coatings with molecularly imprinted TiO₂ for uric acid recognition

Abstract

Combining the surface modification and molecular imprinting technique, a novel piezoelectric sensing platform with excellent molecular recognition capability was established for the detection of uric acid (UA) based on the immobilization of TiO2 nanoparticles onto quartz crystal microbalance (QCM) electrode and modification of molecularly imprinted TiO2 (MIT) layer on TiO2 nanoparticles. The performance of the fabricated biosensor was evaluated, and the results indicated that the biosensor exhibited high sensitivity in UA detection, with a linear range from 0.04 to 45 μM and a limit of detection of 0.01 μM. Moreover, the biosensor presented high selectivity towards UA in comparison with other interferents. The analytical application of the UA biosensor confirmed the feasibility of UA detection in urine sample.

Molecular Imprinting Technology for Determination of Uric Acid

“…Similar effect was described in the case of the formation of the uric acid imprints in TiO 2 [ 51 ]. The MIT modified TiO 2 with uric acid imprints was used in piezoelectric measurements and exhibited significantly higher sensitivity to uric acid than that for interfering molecules such as ascorbic acid, urea, glucose, glutamic acid, purine, or cytosine.…”

“…The transport of charge across the interface between an electrolyte and an electrode is affected by various processes and factors, which are the research object of electrochemical methods, while the research object of quartz crystal microbalance (QCM) is the measurement of a mass variation per unit area by measuring the change in frequency of a quartz crystal resonator. Sometimes a reasonable combination of QCM and electrochemical methods can be applied for simultaneous determination of both signals and this combination is called the electrochemical quartz crystal microbalance (EQCM) [ 12 , 25 , 48 , 51 ].…”

“…A piezoelectric sensor was established for the uric acid detection [ 51 ]. A surface modification and MIT were combined.…”

Selective non-enzymatic uric acid sensing in the presence of dopamine: electropolymerized poly-pyrrole modified with a reduced graphene oxide/PEDOT:PSS composite

“…59,60,62,66,67 Other uric acid sensors based on MIPs exhibited greater efficiency in terms of their linear ranges and detection limits. 7,[63][64][65]68 However, this newly proposed sensor showed a wider linear range for uric acid concentration in the presence of interference molecules, i.e. dopamine, as well as a lower detection limit associated with good selectivity from the imprinted polymer embedded in the electrode surface.…”

Microwave-assisted synthesis of an RGO/CdS/TiO2step-scheme with exposed TiO2{001} facets and enhanced visible photocatalytic activity

“…TiO 2 is a traditional semiconductor photocatalyst which has been widely applied in photocatalysis, solar cells, self-cleaning technologies and biosensors because of its excellent photocatalytic activity, high chemical stability, wide availability and environmental friendliness. [7][8][9][10] However, TiO 2 is subject to many limitations in photocatalytic performance such as weak absorption in the visible light region because of its wide band gap (3.2 eV) and it has low quantum efficiency because of the recombination of photogenerated electron-hole pairs. 11,12 The photocatalytic properties of TiO 2 have been shown to be affected via its shape, size, morphology and specic surface area of TiO 2 .…”