Modification of PEG reduces the immunogenicity of biosynthetic gas vesicles

Wang, Yuanyuan; Fu, Meijun; Yang, Yanlin; Zhang, Jinghan; Zhang, Zhaomeng; Xiao, Jingling; Zhou, Yingjie; Yan, Fei

doi:10.3389/fbioe.2023.1128268

Cited by 10 publications

(8 citation statements)

References 34 publications

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“…However, it is noteworthy that GVs, composed of protein shells synthesized by bacteria, have a certain immunogenicity and can induce an immune response in mice. In our previous study, 46 PEG-GVs were successfully synthesized by PEG-coated GVs, and it was found that PEG-GVs greatly reduced the immunogenicity and reticuloendothelial system (RES) uptake, which provided a new idea for the clinical transformation of GVs in molecular imaging and disease diagnosis and treatment in the future.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound Molecular Imaging of Bladder Cancer via Extradomain B Fibronectin-Targeted Biosynthetic GVs

Feng,

Hao,

Wang

et al. 2023

IJN

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Purpose Ultrasound molecular imaging (UMI) has proven promising to diagnose the onset and progression of diseases such as angiogenesis, inflammation, and thrombosis. However, microbubble-based acoustic probes are confined to intravascular targets due to their relatively large particle size, greatly reducing the application value of UMI, especially for extravascular targets. Extradomain B fibronectin (ED-B FN) is an important glycoprotein associated with tumor genesis and development and highly expressed in many types of tumors. Here, we developed a gas vesicles (GVs)-based nanoscale acoustic probe (ZD2-GVs) through conjugating ZD2 peptides which can specially target to ED-B FN to the biosynthetic GVs. Materials and Methods ED-B FN expression was evaluated in normal liver and tumor tissues with immunofluorescence and Western blot. ZD2-GVs were prepared by conjugating ZD2 to the surface of GVs by amide reaction. The inverted microscope was used to analyze the targeted binding capacity of ZD2-GVs to MB49 cells (bladder cancer cell line). The contrast-enhanced imaging features of GVs, non-targeted control GVs (CTR-GVs), and targeted GVs (ZD2-GVs) were compared in three MB49 tumor mice. The penetration ability of ZD2-GVs in tumor tissues was assessed by fluorescence immunohistochemistry. The biosafety of GVs was evaluated by CCK8, blood biochemistry, and HE staining. Results Strong ED-B FN expression was observed in tumor tissues while little expression in normal liver tissues. The resulting ZD2-GVs had only 267.73 ± 2.86 nm particle size and exhibited excellent binding capability to the MB49 tumor cells. The in vivo UMI experiments showed that ZD2-GVs produced stronger and longer retention in the BC tumors than that of the non-targeted CTR-GVs and GVs. Fluorescence immunohistochemistry confirmed that ZD2-GVs could penetrate the tumor vascular into the interstitial space of the tumors. Biosafety analysis revealed there was no significant cytotoxicity to these tested mice. Conclusion Thus, ZD2-GVs can function as a potential UMI probe for the early diagnosis of bladder cancer.

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Section: Discussionmentioning

confidence: 99%

Ultrasound Molecular Imaging of Bladder Cancer via Extradomain B Fibronectin-Targeted Biosynthetic GVs

Feng,

Hao,

Wang

et al. 2023

IJN

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“…Firstly, it improves the colloidal stability of nanoparticles, promoting their long-term dispersion in a liquid medium. Secondly, using PEG reduces the immunogenicity of nanoparticles, minimizing the potential immune response upon their introduction into a biological system [ 109 ]. Finally, PEG modification prolongs the circulation time of nanoparticles in vivo, enhancing their therapeutic effectiveness.…”

Section: Research On Polysaccharide-aunps As Drug Delivery Carriersmentioning

confidence: 99%

Research Progress of Polysaccharide-Gold Nanocomplexes in Drug Delivery

Song,

Aipire,

Dilxat

et al. 2024

Pharmaceutics

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Clinical drug administration aims to deliver drugs efficiently and safely to target tissues, organs, and cells, with the objective of enabling their therapeutic effects. Currently, the main approach to enhance a drug’s effectiveness is ensuring its efficient delivery to the intended site. Due to the fact that there are still various drawbacks of traditional drug delivery methods, such as high toxicity and side effects, insufficient drug specificity, poor targeting, and poor pharmacokinetic performance, nanocarriers have emerged as a promising alternative. Nanocarriers possess significant advantages in drug delivery due to their size tunability and surface modifiability. Moreover, nano-drug delivery systems have demonstrated strong potential in terms of prolonging drug circulation time, improving bioavailability, increasing drug retention at the tumor site, decreasing drug resistance, as well as reducing the undesirable side effects of anticancer drugs. Numerous studies have focused on utilizing polysaccharides as nanodelivery carriers, developing delivery systems based on polysaccharides, or exploiting polysaccharides as tumor-targeting ligands to enhance the precision of nanoparticle delivery. These types of investigations have become commonplace in the academic literature. This review aims to elucidate the preparation methods and principles of polysaccharide gold nanocarriers. It also provides an overview of the factors that affect the loading of polysaccharide gold nanocarriers with different kinds of drugs. Additionally, it outlines the strategies employed by polysaccharide gold nanocarriers to improve the delivery efficiency of various drugs. The objective is to provide a reference for further development of research on polysaccharide gold nanodelivery systems.

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“…There is another research synthesizing ROS‐responsive prodrug by linking PTX with polyethylene glycol (PEG) chains via thioketal linkers and then co‐assembling with Ce6 to realize combined antitumor efficacy. [ 63 ]…”

Section: Ce6‐based Self‐assembled Nanoparticles For Combinational The...mentioning

confidence: 99%

Ce6 nanoassemblies: Molecular mechanism and strategies for combinational anticancer therapy

Liu,

Xu,

Lyu

et al. 2023

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Nowadays, cancer has become the leading cause of death worldwide, driving the need for effective therapeutics to improve patient prognosis. Photodynamic therapy (PDT) has been widely applied as an antitumor modality, owing to its minimal invasiveness, localized tumor damage, and high safety profile. However, its efficacy is limited by poor stability of photosensitizers, inadequate tumor accumulation, and a complex tumor microenvironment. To overcome these challenges, extensive endeavors have been made to explore the co‐assembly of the widely used photosensitizer chlorin e6 (Ce6) with various functional small molecules to enhance pharmacodynamic activity. This review provides a comprehensive overview of current studies on Ce6‐based nanoparticles for effective PDT and precise delivery of functional molecules. The self‐assembly mechanism will be discussed in detail, with a focus on potential strategies for combinational therapy with PDT.

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Modification of PEG reduces the immunogenicity of biosynthetic gas vesicles

Cited by 10 publications

References 34 publications

Ultrasound Molecular Imaging of Bladder Cancer via Extradomain B Fibronectin-Targeted Biosynthetic GVs

Ultrasound Molecular Imaging of Bladder Cancer via Extradomain B Fibronectin-Targeted Biosynthetic GVs

Research Progress of Polysaccharide-Gold Nanocomplexes in Drug Delivery

Ce6 nanoassemblies: Molecular mechanism and strategies for combinational anticancer therapy

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