Modification of the<i>in vitro</i>Cytotoxicity of Hydrogen Peroxide by Iron Complexes

Jonas, S. K.; Riley, Patrick A.

doi:10.3109/10715769209083145

Cited by 17 publications

(4 citation statements)

References 28 publications

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“…This would suggest that the presence of iron within the cell poses more risk than any effect on free radical generation in the external milieu. Evidence to support this hypothesis is provided by tissue culture studies showing that the effects of exposure of cells to hydrogen peroxide are ameliorated by the presence of iron in a nonabsorbable form but exacerbated when iron is presented in an absorbable form (24). Although body iron stores were not measured in this study, we found higher concentration of liver iron in our earlier short-term study (17), which suggests that there would have been higher circulating concentrations of iron in this study.…”

Section: Discussioncontrasting

confidence: 45%

Chronic Exposure to High Levels of Dietary Iron Fortification Increases Lipid Peroxidation in the Mucosa of the Rat Large Intestine

Lund

Fairweather‐Tait

Wharf

et al. 2001

The Journal of Nutrition

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There is increasing evidence that excess dietary iron may be a risk factor for colorectal cancer. However, the majority of animal studies looking at possible mechanism have used unrealistically high concentrations of iron. The current study was designed to test whether chronic exposure to high levels of iron fortification affects the free radical generating capacity of the lumenal contents, mucosal lipid peroxidation and crypt cell proliferation. Rats were fed diets containing either 29 mg/kg or 102 mg/kg of elemental iron for 6 mo. The free radical generating capacity of lumenal contents was assessed using an in vitro assay. Crypt cell proliferation rate was measured in tissues taken from the cecum and colon, with the remaining tissue being used for the assessment of lipid peroxidation. Chronic feeding of iron did not increase crypt cell proliferation rate in either the colon or cecum, but it was associated with an increase in free radical generating capacity in the colon and increased lipid peroxidation, particularly in the cecum. These results may be relevant to epidemiological evidence showing that dietary iron is associated with the risk of proximal colon cancer in humans. J. Nutr. 131: 2928 -2931, 2001.

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Section: Discussioncontrasting

confidence: 45%

Chronic Exposure to High Levels of Dietary Iron Fortification Increases Lipid Peroxidation in the Mucosa of the Rat Large Intestine

Lund

Fairweather‐Tait

Wharf

et al. 2001

The Journal of Nutrition

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“…Conversely, increasing intracellular iron can enhance H 2 O 2 -induced injury as seen with cardiomyocytes [16]. Pre-incubation of the epithelial cell line CNCMI221 with Fe 3+ /8-hydroxyquinoline enhanced the observed cytotoxic effects of H 2 O 2 [17]. It is well known that the primary oxidant generated by the Fenton reaction (H 2 O 2 + Fe 2+ ) is the hydroxyl radical, HO • [18].…”

Section: Introductionmentioning

confidence: 99%

Role of labile iron in the toxicity of pharmacological ascorbate

Wagner

Buettner

et al. 2015

Free Radical Biology and Medicine

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Pharmacological ascorbate has been shown to induce toxicity in a wide range of cancer cell lines; using animal models pharmacological ascorbate has shown promise for use in cancer treatment. At pharmacological concentrations the oxidation of ascorbate produces a high flux of H2O2 via the formation of ascorbate radical (Asc•−). The rate of oxidation of ascorbate is principally a function of the level of catalytically active metals. Iron in cell culture media contributes significantly to the rate of H2O2 generation. We hypothesized that increasing intracellular iron would enhance ascorbate-induced cytotoxicity and that iron chelators could modulate the catalytic efficiency with respect to ascorbate oxidation. Treatment of cells with the iron-chelators deferoxamine (DFO) or dipyridyl (DPD) in the presence of 2 mM ascorbate decreased the flux of H2O2 generated by pharmacological ascorbate and reversed ascorbate-induced toxicity. Conversely, increasing the level of intracellular iron by pre-incubating cells with Fe-hydroxyquinoline (HQ) increased ascorbate toxicity and decreased clonogenic survival. These findings indicate that redox metal metals, e.g. Fe3+/Fe2+, have an important role in ascorbate-induced cytotoxicity. Approaches that increase catalytic iron could potentially enhance the cytotoxicity of pharmacological ascorbate in vivo.

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“…The primary cause of radical hydroxyl damage is H 2 O 2 . The quantity of hydroxyl radicals in the body is partially controlled by the cellular antioxidant status (Jonas & Riley, 1992). Cells have their antioxidant types of machinery, which consist of several Phase II detoxification enzymes like glutathione peroxidases (GPXs) and the three superoxide dismutase isoforms (SOD1, SOD2, SOD3) (Masutani & Thioredoxin, 2002).…”

Section: Oxidative Stressmentioning

confidence: 99%

The prophylactic roles of dietary antioxidants for medical radiology workers: A mini-review

Khattab¹,

Sayed²,

Altaf³

et al. 2022

NRFHH

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Antioxidants play a very crucial naturally role in our bodies. Still, unfortunately, that is not enough. We need to compensate for the shortage we face by increasing the dietary antioxidants as exogenous sources, especially when there is an exogenous factor-induced oxidative stress as with the medical radiology staff because how much ionizing radiation they are exposed to exceeds the primitive dose limit by the International Commission on Radiological Protection (ICRP). Thus, a suitable uptake of minerals, vitamins, and Combinations of dietary antioxidants through the daily diet and balanced it remains the promising approach without exceeding the uptakes doses that could reverse the positive effects of this approach. This review focuses on the present knowledge about dietary antioxidant sources, mechanisms, effects of the cell components. It critically investigates studies examining the effects of dietary antioxidant supplementation and its role among medical radiology workers.

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Modification of thein vitroCytotoxicity of Hydrogen Peroxide by Iron Complexes

Cited by 17 publications

References 28 publications

Chronic Exposure to High Levels of Dietary Iron Fortification Increases Lipid Peroxidation in the Mucosa of the Rat Large Intestine

Chronic Exposure to High Levels of Dietary Iron Fortification Increases Lipid Peroxidation in the Mucosa of the Rat Large Intestine

Role of labile iron in the toxicity of pharmacological ascorbate

The prophylactic roles of dietary antioxidants for medical radiology workers: A mini-review

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