Modified AFLP analysis method for species with small genomes

James, C. M.; Lesemann, Silke

doi:10.1007/bf02772806

Cited by 7 publications

(7 citation statements)

References 5 publications

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“…Cell colonies were successfully formed and AFLP markers have proved to be highly polymorphic and reproducible, as reported by James et al (2003), and gel replication confirmed results. That was expected, since AFLP has been useful in detecting chromosome losses and in revealing more information about hybridity in somatic hybrids, because it provides reliable markers, high resolution and efficiency .…”

Section: Resultssupporting

confidence: 72%

“…The ability to use a wide range of irradiation (50 to 200 Gy) is of interest, because modulation of irradiation dosage may be used, as stated by Kalavacharla et al (2006), to construct maps with varying levels of resolution, as different protoplast fusion events may create a multitude of different hybrid cell panels which may be arranged to cover a whole genome in a radiation map.…”

Section: Resultsmentioning

confidence: 99%

“…In their study, validation of the co-retention of physically linked markers was obtained by analysis of a pair of closely linked barley markers which were either both present or absent in the barley-tobacco hybrids, consistent with tight linkage, demonstrating the utility of the panel for exploitation in mapping procedures. Retention was also achieved by Kalavacharla et al (2006), who presented a high-resolution radiation hybrid map of wheat chromosome 1D, and by Kynast et al (2002), who demonstrated the use of maize radiation hybrid lines for physical mapping, and related genetic map distances to physical chromosome segment sizes.…”

Section: Resultsmentioning

confidence: 99%

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Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus

Bona

Stelly²,

Miller³

et al. 2009

Pesq. agropec. bras.

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-The objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion) to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes.

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Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus

Bona

Stelly²,

Miller³

et al. 2009

Pesq. agropec. bras.

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show abstract

“…The AFLP technique was originally developed in plants with genome size ranging from 1 Â 10 8 to 6 Â 10 9 bp. Thus, it is an appropriate technique for Humulus lupulus L. since its genome size is 1 Â 10 9 bp (James et al, 2003). On the other hand, a measure of stability in the DNA methylation is needed due to its effect in the timing of DNA replication, the chromatin structure, the mutation frequency, and as a basis for epigenetic phenomena (Finnegan et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic and epigenetic variation in hop plants regenerated from sequential subcultures of organogenic calli

Peredo

Revilla

Arroyo-García³

2006

Journal of Plant Physiology

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“… Box 2 Strategies and modifications Before embarking on AFLP, it is however worth checking some of the variant AFLP protocols (Lindstedt et al . 2000; van der Wurff et al .…”

mentioning

confidence: 99%

Ten years of AFLP in ecology and evolution: why so few animals?

2005

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Researchers in the field of molecular ecology and evolution require versatile and low-cost genetic typing methods. The AFLP (amplified fragment length polymorphism) method was introduced 10 years ago and shows many features that fulfil these requirements. With good quality genomic DNA at hand, it is relatively easy to generate anonymous multilocus DNA profiles in most species and the start-up time before data can be generated is often less than a week. Built-in dynamic, yet simple modifications make it possible to find a protocol suitable to the genome size of the species and to screen thousands of loci in hundreds of individuals for a relatively low cost. Until now, the method has primarily been applied in studies of plants, bacteria and fungi, with a strong bias towards economically important cultivated species and their pests. In this review we identify a number of research areas in the study of wild species of animals where the AFLP method, presently very much underused, should be a very valuable tool. These aspects include classical problems such as studies of population genetic structure and phylogenetic reconstructions, and also new challenges such as finding markers for genes governing adaptations in wild populations and modifications of the protocol that makes it possible to measure expression variation of multiple genes (cDNA-AFLP) and the distribution of DNA methylation. We hope this review will help molecular ecologists to identify when AFLP is likely to be superior to other more established methods, such as microsatellites, SNP (single nucleotide polymorphism) analyses and multigene DNA sequencing.

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Modified AFLP analysis method for species with small genomes

Cited by 7 publications

References 5 publications

Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus

Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus

Assessment of genetic and epigenetic variation in hop plants regenerated from sequential subcultures of organogenic calli

Ten years of AFLP in ecology and evolution: why so few animals?

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