Modified beacon probe assisted dual signal amplification for visual detection of microRNA

Sun, Xiuwei; Ying, Na; Ju, Chuanjing; Li, Zhongyi; Xu, Na; Qu, Guijuan; Liu, Wensen; Wan, Jiayu

doi:10.1016/j.ab.2018.04.010

Cited by 9 publications

(3 citation statements)

References 8 publications

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“…35 The CHA-based amplification techniques are attracting more attention owing to their advantages as enzyme-free, highly efficient, and isothermal amplification methods. 36–39 Hence, we designed an innovative biotinylated trigger release (BTR) probe. This BTR probe was divided into a recognition region for targeting miRNA and a catalyst region for the CHA reaction trigger.…”

Section: Introductionmentioning

confidence: 99%

A duplex-specific nuclease (DSN) and catalytic hairpin assembly (CHA)-mediated dual amplification method for miR-146b detection

Han

Zeng

et al. 2023

Analyst

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Section: Introductionmentioning

confidence: 99%

A duplex-specific nuclease (DSN) and catalytic hairpin assembly (CHA)-mediated dual amplification method for miR-146b detection

Han

Zeng

et al. 2023

Analyst

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“…To achieve better LODs, many research efforts have focused on integration of these amplification processes with new materials (as signal enhancers) or detection platforms . Some studies have described attempts to combine or cascade different amplification assays together, such as CHA + HCR, CHA + RCA, SDA + RCA, and DSN + HCR . The LODs of each of these combined assays can be down to fM, or even aM levels.…”

mentioning

confidence: 99%

“…10 Some studies have described attempts to combine or cascade different amplification assays together, such as CHA + HCR, 27 CHA + RCA, 28 SDA + RCA, 29 and DSN + HCR. 30 The LODs of each of these combined assays can be down to fM, or even aM levels. Unfortunately, these combinations also prolong detection times, especially for time-consuming recycling amplification processes, which require several hours.…”

mentioning

confidence: 99%

Magnetic Three-Phase Single-Drop Microextraction for Rapid Amplification of the Signals of DNA and MicroRNA Analysis

Tang

Yao

et al. 2020

Anal. Chem.

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The detection of nucleic acids usually suffers from a lengthy amplification process. To obtain an enhanced signal within several seconds, a magnetic three-phase single-drop microextraction (MTP-SDME) approach was developed for the quantification of nucleic acids. First, a target-triggered recycling amplification strategy was used to constitute magnetic branched DNA/Fe3O4 networks, which displayed peroxidase-like catalytic activity toward the 3,3′,5,5′-tetramethylbenzidine colorimetric reaction. The networks were separated and enriched by rapid (6 s) MTP-SDME (with only 6 μL of solvent required), thereby producing highly sensitive signals for the quantification of nucleic acids. The signals were significantly amplified by the triple strategy (network formation, MTP-SDME, and catalytic reaction). The application of magnetic extraction minimized the background signal, avoided sample matrix effects, and enhanced the analyte signals. This assay achieved linear calibration curves of between 0.5 aM and 1 pM for microRNA-122 (miRNA-122) and between 1 aM and 1 pM for HBV-T (a DNA fragment from hepatitis B virus). Limits of detection of 0.15 aM for miRNA-122 and 0.34 aM for HBV-T were attained, with relative standard deviations of <5.0% (n = 3). Furthermore, the procedure was applied to determine miRNA-122 and HBV-T in genuine serum samples from hepatocellular carcinoma patients.

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An aggregation-induced emission fluorogen/DNA probe carrying an endosome escaping pass for tracking reduced thiol compounds in cells

Cao

Guo

et al. 2020

Anal Bioanal Chem

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Modified beacon probe assisted dual signal amplification for visual detection of microRNA

Cited by 9 publications

References 8 publications

A duplex-specific nuclease (DSN) and catalytic hairpin assembly (CHA)-mediated dual amplification method for miR-146b detection

A duplex-specific nuclease (DSN) and catalytic hairpin assembly (CHA)-mediated dual amplification method for miR-146b detection

Magnetic Three-Phase Single-Drop Microextraction for Rapid Amplification of the Signals of DNA and MicroRNA Analysis

An aggregation-induced emission fluorogen/DNA probe carrying an endosome escaping pass for tracking reduced thiol compounds in cells

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