Modified Benzidine Test for the Detection of Cytochrome-Containing Respiratory Systems in Microorganisms

Deibel, R. H.; Evans, James B.

doi:10.1128/jb.79.3.356-360.1960

Cited by 127 publications

(38 citation statements)

References 13 publications

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“…Another clue to the anaerobic nature of the mutant was suggested by its negative benzidine test. This test was originally used to confirm the absence of cytochromes in anaerobic organisms such as Streptococcus and Clostridium (9). Confirmation of the anaerobic nature of the mutant was obtained from several experiments.…”

Section: Physiological Characterization Of the Res-strainmentioning

confidence: 99%

Characterization of Gentamicin‐Resistant Respiratory‐Deficient (Res⁻) Variant Strains of Staphylococcus aureus

Lewis

Bharosay

et al. 1990

Microbiology and Immunology

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Exposure of sensitive cells of Staphylococcus aureus to concentrations of gentamicin higher than the minimal inhibitory concentration, results in the recovery of low level resistant strains with a greatly altered phenotype (variants). Because the phenotypic alteration in these strains is so great the expected diagnostic characterization of these variants as S. aureus is obscured. Starting with a geneticallymarked parent strain, a comprehensive cytological, physiological, morphological , genetic and biochemical analysis of the variants isolated from it was carried out. The genetic lineage of the variants to the parent was also established by DNA/DNA hybridization. Variants result from mutations in the hemin biosynthesis locus , the effect of which is to disrupt the synthesis of components of the electron transport system, lipid synthesis and selected nucleotide synthesis. Thus the strains are defective in aerobic and anaerobic respiration, (res-), in active transport of aminoglycosides (which confers low level resistance), export of characteristic exo-enzymes, and in cell wall composition and structure.

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Section: Physiological Characterization Of the Res-strainmentioning

confidence: 99%

Characterization of Gentamicin‐Resistant Respiratory‐Deficient (Res⁻) Variant Strains of Staphylococcus aureus

Lewis

Bharosay

et al. 1990

Microbiology and Immunology

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“…Benzidine test-by the method of Deibel & Evans (1960). Growth i n MRS or RCM broth was tested at 23", the pH ~a l u e being determined after 8-15 days by capillator.…”

Section: Identification Of Gram Positive Nonsporing Bacteriamentioning

confidence: 99%

Microbial Flora of Raw and Pasteurized Egg Albumen

Barnes

Corry

1969

Journal of Applied Bacteriology

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Summary Gram negative bacteria were the main spoilage organisms of raw albumen stored at 5, 10, 15 or 20° and were identified as strains of pigmented and nonpigmented Pseudomonas, Acinetobacter and Enterobacter liquefaciens. Only one unidentified Gram negative motile rod which failed to attack glucose was found to survive pasteurization in egg albumen (pH 9‐1) at 57.2° for 3 min. Other Gram negative bacteria isolated from one batch of stored albumen were shown to be heat sensitive, so that recontamination had probably occurred after pasteurization. Most of the organisms which survived pasteurization were found to be faecal streptococci, strains resembling Aerococcus viridans, Microbacterium lacticum and two groups (B and C) of unidentified catalase negative rods, or coccobacilli which in many respects resembled the atypical lactobacilli of Thornley & Sharpe (1959). Of these strains only the faecal streptococci and groups B and C were isolated from stored albumen. The effect of the spoilage flora on the ‘catalase’ activity of albumen before and after pasteurization has been studied in relation to the possibility of a ‘catalase’ test being used to monitor the pasteurization process.

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“…which we did not detect, the main bacterial contaminants of wort and beer found by Ault (1965) and Kleyn & Hough (1971) were present in the South African lager brewery. The value of the diagnostic scheme of Ault (1965), modified by use of the benzidine test (Deibel & Evans 1960) for the recognition of lactobacilli and pediococci, the medium of Carr (1968) to distinguish between those acetic acid bacteria that oxidized acetate and those that did not, and the API 20E system for the identification of enterobacteria, was demonstrated in that with a few easily performed tests all isolates could be assigned to one of the above groups. The benzidine test was shown to be essential; without it certain catalase positive isolates of Pediococcus spp.…”

Section: Resultsmentioning

confidence: 99%

Distribution of Bacterial Contaminants in a South African Lager Brewery

Vuuren

Loos

Louw

et al. 1979

Journal of Applied Bacteriology

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Bacteria isolated from contaminated pitching yeast, fermenting wort and beer samples from a South African lager brewery over a one‐year period were tentatively identified by an improved, rapid diagnostic procedure as pediococci (41%), homofermentative lactobacilli (5%), heterofermentative lactobacilli (9%), Acetobacter spp. (7%), Gluconobacter spp. (13%) and Hafnia protea (25%). Pediococci and lactobacilli dominated samples taken from fermentation, storage and ‘bright’ beer tanks but were absent from pitched wort samples, from collection vessels and the single pitching yeast sample investigated. Acetic acid bacteria and H. protea were widely distributed in collection vessel, fermentation and storage tank samples, and H. protea was isolated from recycled pitching yeast.

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Modified Benzidine Test for the Detection of Cytochrome-Containing Respiratory Systems in Microorganisms

Cited by 127 publications

References 13 publications

Characterization of Gentamicin‐Resistant Respiratory‐Deficient (Res⁻) Variant Strains of Staphylococcus aureus

Characterization of Gentamicin‐Resistant Respiratory‐Deficient (Res⁻) Variant Strains of Staphylococcus aureus

Microbial Flora of Raw and Pasteurized Egg Albumen

Distribution of Bacterial Contaminants in a South African Lager Brewery

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Modified Benzidine Test for the Detection of Cytochrome-Containing Respiratory Systems in Microorganisms

Cited by 127 publications

References 13 publications

Characterization of Gentamicin‐Resistant Respiratory‐Deficient (Res−) Variant Strains of Staphylococcus aureus

Characterization of Gentamicin‐Resistant Respiratory‐Deficient (Res−) Variant Strains of Staphylococcus aureus

Microbial Flora of Raw and Pasteurized Egg Albumen

Distribution of Bacterial Contaminants in a South African Lager Brewery

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Characterization of Gentamicin‐Resistant Respiratory‐Deficient (Res⁻) Variant Strains of Staphylococcus aureus

Characterization of Gentamicin‐Resistant Respiratory‐Deficient (Res⁻) Variant Strains of Staphylococcus aureus