Modified <i>CAROTENOID CLEAVAGE DIOXYGENASE8</i> expression correlates with altered branching in kiwifruit (<i>Actinidia chinensis</i>)

Ledger, Susan E.; Janssen, Bart J.; Karunairetnam, Sakuntala; Wang, Tianchi; Snowden, Kimberley C.

doi:10.1111/j.1469-8137.2010.03394.x

Cited by 74 publications

(63 citation statements)

References 59 publications

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“…Mutants in SL biosynthesis and signaling increase branching in diverse plant species, including Arabidopsis, pea, and petunia (for review, see Goulet and Klee, 2010), as well as tomato (Solanum lycopersicum; Vogel et al, 2010), kiwifruit (Actinidia deliciosa; Ledger et al, 2010), and rice (Wang and Li, 2011). In contrast with the prevailing model, which places SLs upstream of Tb1 orthologs in a linear pathway of branching control, our results indicate that Tb1 mediates a branchingspecific subnetwork that has become independent of SL signaling in maize.…”

Section: Discussioncontrasting

confidence: 52%

Diverse Roles of Strigolactone Signaling in Maize Architecture and the Uncoupling of a Branching-Specific Subnetwork

et al. 2012

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Strigolactones (SLs) control lateral branching in diverse species by regulating transcription factors orthologous to Teosinte branched1 (Tb1). In maize (Zea mays), however, selection for a strong central stalk during domestication is attributed primarily to the Tb1 locus, leaving the architectural roles of SLs unclear. To determine how this signaling network is altered in maize, we first examined effects of a knockout mutation in an essential SL biosynthetic gene that encodes CAROTENOID CLEAVAGE DIOXYGENASE8 (CCD8), then tested interactions between SL signaling and Tb1. Comparative genome analysis revealed that maize depends on a single CCD8 gene (ZmCCD8), unlike other panicoid grasses that have multiple CCD8 paralogs. Function of ZmCCD8 was confirmed by transgenic complementation of Arabidopsis (Arabidopsis thaliana) max4 (ccd8) and by phenotypic rescue of the maize mutant (zmccd8::Ds) using a synthetic SL (GR24). Analysis of the zmccd8 mutant revealed a modest increase in branching that contrasted with prominent pleiotropic changes that include (1) marked reduction in stem diameter, (2) reduced elongation of internodes (independent of carbon supply), and (3) a pronounced delay in development of the centrally important, nodal system of adventitious roots. Analysis of the tb1 zmccd8 double mutant revealed that Tb1 functions in an SL-independent subnetwork that is not required for the other diverse roles of SL in development. Our findings indicate that in maize, uncoupling of the Tb1 subnetwork from SL signaling has profoundly altered the balance between conserved roles of SLs in branching and diverse aspects of plant architecture.

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Section: Discussioncontrasting

confidence: 52%

Diverse Roles of Strigolactone Signaling in Maize Architecture and the Uncoupling of a Branching-Specific Subnetwork

et al. 2012

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“…Although D27-like proteins are found throughout cyanobacteria and green algae, two clades are strictly specific to land plants. Notably, CCD7 (MAX3) and CCD8 (MAX4) form monophyletic clades in land plants, and putative orthologs of both CCD7 and CCD8 are present in Chlamydomonas reinhardtii and other photosynthetic eukaryotes (Ledger et al, 2010;Vallabhaneni et al, 2010;Proust et al, 2011). Thus, the enzymatic machinery necessary for the early steps of SL biosynthesis may have been present in the ancestors of the land plants.…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis Ortholog of Rice DWARF27 Acts Upstream of MAX1 in the Control of Plant Development by Strigolactones

et al. 2012

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Strigolactones (SLs) are carotenoid-derived plant hormones that regulate shoot branching, secondary growth, root development, and responses to soil phosphate. In Arabidopsis (Arabidopsis thaliana), SL biosynthesis requires the sequential action of two carotenoid cleavage dioxygenases, MORE AXILLARY GROWTH3 (MAX3) and MAX4, followed by a cytochrome P450, MAX1. In rice (Oryza sativa), the plastid-localized protein DWARF27 (OsD27) is also necessary for SL biosynthesis, but the equivalent gene in Arabidopsis has not been identified. Here, we use phylogenetic analysis of D27-like sequences from photosynthetic organisms to identify AtD27, the likely Arabidopsis ortholog of OsD27. Using reverse genetics, we show that AtD27 is required for the inhibition of secondary bud outgrowth and that exogenous application of the synthetic SL GR24 can rescue the increased branching phenotype of an Atd27 mutant. Furthermore, we use grafting to demonstrate that AtD27 operates on a nonmobile precursor upstream of MAX1 in the SL biosynthesis pathway. Consistent with the plastid localization of OsD27, we also show that AtD27 possesses a functional plastid transit peptide. We demonstrate that AtD27 transcripts are subject to both local feedback and auxin-dependent signals, albeit to a lesser extent than MAX3 and MAX4, suggesting that early steps in SL biosynthesis are coregulated at the transcriptional level. By identifying an additional component of the canonical SL biosynthesis pathway in Arabidopsis, we provide a new tool to investigate the regulation of shoot branching and other SL-dependent developmental processes.

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“…It has long been called "the king of fruits" because of its remarkably high vitamin C content and balanced nutritional composition of minerals, dietary fiber, and other beneficial metabolites (Huang et al, 2013). Extensive studies on the metabolic accumulation of vitamin C, carotenoids, and flavonoids have been conducted in kiwifruit (Ampomah-Dwamena et al, 2009;Ledger et al, 2010;Montefiori et al, 2011;Pilkington et al, 2012;Zuo et al, 2012;Park et al, 2013). Kiwifruits are now grown in Italy, Chile, France, and the United States, among other countries (Huang and Ferguson, 2001).…”

Section: Introductionmentioning

confidence: 99%

Production of transgenic kiwifruit plants harboring the SbtCry1Ac gene

Zhang

Liu

2015

Genet. Mol. Res.

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ABSTRACT. The kiwifruit (Actinidia chinensis Planch.) is an economically and nutritionally important fruit crop that has a remarkably high vitamin C content and is popular throughout the world. However, kiwifruit plants are vulnerable to attack from pests, and effective pest control is urgently required. Transgenic kiwifruit plants containing the synthetic chimeric gene SbtCry1Ac that encodes the insecticidal protein btCrylAc were obtained through an Agrobacterium-mediated transformation of kiwifruit leaf discs. The kanamycin resistance of the transgenic plants was then analyzed. Results from polymerase chain reactions and genomic DNA Southern blot analyses indicated that SbtCrylAc had been integrated into the genomes of these plants. The results of insect bioassays revealed that the average Oraesia excavate inhibition rate of plants tested at 10 days post-infestation was 75.2%. To our knowledge, this is the first study that has developed insect-resistant transgenic kiwifruit plants.

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Modified CAROTENOID CLEAVAGE DIOXYGENASE8 expression correlates with altered branching in kiwifruit (Actinidia chinensis)

Cited by 74 publications

References 59 publications

Diverse Roles of Strigolactone Signaling in Maize Architecture and the Uncoupling of a Branching-Specific Subnetwork

Diverse Roles of Strigolactone Signaling in Maize Architecture and the Uncoupling of a Branching-Specific Subnetwork

The Arabidopsis Ortholog of Rice DWARF27 Acts Upstream of MAX1 in the Control of Plant Development by Strigolactones

Production of transgenic kiwifruit plants harboring the SbtCry1Ac gene

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