Production of transgenic kiwifruit plants harboring the SbtCry1Ac gene

Zhang, Hanyao; Liu, H M; Liu, X Z

doi:10.4238/2015.july.28.16

Cited by 14 publications

(7 citation statements)

References 16 publications

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“…Natural as well as synthetic insect resistance genes had been transferred into a number of horticultural crops for imparting resistance against various insect-pests. Zhang et al (2015) transformed kiwifruit plant (Actinidia chinensis) with a synthetic chimeric gene SbtCry1Ac encoding for protein btCry1Ac, When the transgenic plants were screened for insect resistance in insect bioassays, an average of 75.2% Oraesia excavate inhibition rate was reported at 10 days of post-infection. This technology could be highly useful to protect yield losses of kiwifruit due to insect attack, which is an economically as well as nutritionally important fruit crop, offering a remarkably high vitamin C content.…”

Section: Biotic Stress Management Through Transgenic Approachmentioning

confidence: 99%

Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review

et al. 2017

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Genetic engineering technique offers myriads of applications in improvement of horticultural crops for biotic and abiotic stress tolerance, and produce quality enhancement. During last two decades, a large number of transgenic horticultural crops has been developed and more are underway. A number of genes including natural and synthetic Cry genes, protease inhibitors, trypsin inhibitors and cystatin genes have been used to incorporate insect and nematode resistance. For providing protection against fungal and bacterial diseases, various genes like chitinase, glucanase, osmotin, defensin and pathogenesis-related genes are being transferred to many horticultural crops world over. RNAi technique has been found quite successful in inducing virus resistance in horticultural crops in addition to coat protein genes. Abiotic stresses such as drought, heat and salinity adversely affect production and productivity of horticultural crops and a number of genes encoding for biosynthesis of stress protecting compounds including mannitol, glycine betaine and heat shock proteins have been employed for abiotic stress tolerance besides various transcription factors like DREB1, MAPK, WRKY, etc. Antisense gene and RNAi technologies have revolutionized the pace of improvement of horticultural crops, particularly ornamentals for color modification, increasing shelf-life and reducing post-harvest losses. Precise genome editing tools, particularly CRISPR/Cas9, have been efficiently applied in tomato, petunia, citrus, grape, potato and apple for gene mutation, repression, activation and epigenome editing. This review provides comprehensive overview to draw the attention of researchers for better understanding of genetic engineering advancements in imparting biotic and abiotic stress tolerance as well as on improving various traits related to quality, texture, plant architecture modification, increasing shelf-life, etc. in different horticultural crops.

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Section: Biotic Stress Management Through Transgenic Approachmentioning

confidence: 99%

Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review

et al. 2017

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“…Kiwifruit (Actinidia deliciosa), which is called 'the king of fruit' in some countries due to its high content of vitamin C, is native to the Yangtze River region in China, even though its improvement and commercial planting began in New Zealand (ZHANG et al, 2015). Kiwifruit has great social and economic importance in several countries, such as New Zealand, Italy and Chile, because they export part of their production, which employs much workforce (HUANG et al, 2013;ZHANG et al, 2015). The world's main kiwifruit producers are China (2,024,603 t/year), Italy (541,150 t/year) and New Zealand (411,783 t/year) (FAO, 2017).…”

Section: Introductionmentioning

confidence: 99%

Alternative budburst inducers for kiwifruit vines grown in an organic system

et al. 2021

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Lack of budburst inducers is a major problem in organic production of temperate fruits. This study aimed at evaluating the effect of alternative budburst inducers on kiwifruit vines of cultivar Bruno grown in an orchard in Pelotas, Rio Grande do Sul (RS), Brazil. Inducers efficiency was investigated in both 2016-2017 and 2017-2018 seasons. The first season comprised of ten treatments, which used garlic extract at different concentrations, both, associated with and without mineral oil; Welsh onion extract at different concentrations, both associated with and without mineral oil; just mineral oil; and control (no application). In the second season, evaluation was carried out ith treatments that performed well in the first season, garlic extract atdifferent concentrations associated with mineral oil, just mineral oil and control. The followingvariables were evaluated: budburst percentage, phenology (budburst and flowering), fruit set, number of fruit/plant, fruit mass/ plant, estimate production, mean mass, fruit length and diameter. Results showed that 10% garlic extract + 2% mineral oil induced the highest budburst percentages in both seasons; the position of buds on branches influences budburst; apical buds exhibit the highest budburst percentage; 15% garlic extract causes phytotoxicity to buds; and Welsh onion extract is inefficient to induce budburst.

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“…The fruit of yellow kiwifruit is very popular in markets (Yuan and Wang, 2018). The output of yellow kiwifruit in New Zealand, Italy, Greece, Chile and other countries increased year by year (Minchin et al, 2003;Zhang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Tetraploidy enhances the cold-resistance in yellow kiwifruit (Actinidia chinensis)

Liu

Zhou

et al. 2020

Preprint

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Yellow kiwifruit ( Actinidia chinensis ) is highly susceptible to severe weather, such as low temperature and frost, which may affect the production in the coming year. And the cold-resistant mechanism of kiwifruit associated with gene regulation is poorly investigated. To botain cold-resistant germplam, to provide insight into the causes of differences in low temperature tolerance due to ploidy and to better understand cold-adaptive mechanisms in tetraploid kiwifruit, the diploid yellow kiwifruit ‘SWFU03’ and its tetraploid plantlets were subjected to cold-tolerant screening with L-hydroxyproline (L-Hyp) and low temperature, the selected ones were then analyzed by transcriptome data and confirmed by RT-qPCR. The results showed that the survival rate of tetraploid plants was 62.22% when treated with 8 mmol/L L-Hyp for 30 days, while all the diploid ones died. After treated with 0°C for 12 h, then at room temperature for seven days, the survival rate of tetraploid plantlets was 42.22%, while all diploidy died. Hence, cold tolerance of the tetraploid plantlets was stronger than that of the diploid genotypes. Using these two screening systems, 126 cold-resistant tetraploid tissue culture plantlets were obtained. A total of 1630 differentially expressed genes (DEGs) were identified, of which 619 were up-regulated and 1011 were down-regulated in the low temperature treatment goup. The DEGs enriched in the cold-tolerance related pathways mainly included plant hormone signal transduction, and starch and sucrose metabolism pathway. RT-qPCR analysis confirmed the expression levels of eight up-regulated genes in these pathways in the cold-resistant mutants. In conclusion, this study has identified cold-resistant yellow kiwifruit plantlets and cold-tolerance related genes. Moreover, the dataset got in this study advances our knowledge of the cold-adaptive genes in the regulatory networks and leads to understand the cold tolerance mechanisms in the tetraploid yellow kiwifruit.

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Production of transgenic kiwifruit plants harboring the SbtCry1Ac gene

Cited by 14 publications

References 16 publications

Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review

Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review

Alternative budburst inducers for kiwifruit vines grown in an organic system

Tetraploidy enhances the cold-resistance in yellow kiwifruit (Actinidia chinensis)

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