Modified Method of Multilocus Sequence Typing (MLST) for Serotyping in<i>Salmonella</i>Species

Yun, Young Sun; Chae, Su‐Jin; Na, Hye Young; Chung, Gyung Tae; Yoo, Cheon-Kwon; Lee, Deog‐Yong

doi:10.4167/jbv.2015.45.4.314

Cited by 7 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Schemes targeting seven genes are typically considered the “classical” MLST approach; this typing approach was originally proposed for isolates of Neisseria meningitidis (Liu, 2010). In this review, we focus on the most widely used Salmonella scheme targeting seven housekeeping genes [ aroC , dnaN , hemD , hisD , thrA , sucA , and purE ; hereafter denoted as legacy MLST to distinguish newer approaches (described below)] (Li et al, 2009; Yun et al, 2015). It was first introduced for Salmonella Typhi in 2002 (Kidgell et al, 2002), and extended to all Salmonella serovars in 2012 (Achtman et al, 2012; Figure 1).…”

Section: Banding Pattern-based and Sequencing-based Characterization mentioning

confidence: 99%

“…Legacy MLST can deliver results more rapidly than PFGE (Shi et al, 2015; Yun et al, 2015; Table 1), and the publicly available databases and online query system enable legacy MLST results to be highly reproducible and exchangeable between laboratories. However, legacy MLST shows lower discriminatory power than PFGE and MLVA, which limits its application to further discriminate isolates within a given serovar (Torpdahl et al, 2005; Alcaine et al, 2006; Foley et al, 2006; Harbottle et al, 2006; Hauser et al, 2012; Ngoi et al, 2015), and for source attribution (Barco et al, 2013).…”

Section: Banding Pattern-based and Sequencing-based Characterization mentioning

confidence: 99%

See 1 more Smart Citation

Assessment and Comparison of Molecular Subtyping and Characterization Methods for Salmonella

et al. 2019

View full text Add to dashboard Cite

The food industry is facing a major transition regarding methods for confirmation, characterization, and subtyping of Salmonella . Whole-genome sequencing (WGS) is rapidly becoming both the method of choice and the gold standard for Salmonella subtyping; however, routine use of WGS by the food industry is often not feasible due to cost constraints or the need for rapid results. To facilitate selection of subtyping methods by the food industry, we present: (i) a comparison between classical serotyping and selected widely used molecular-based subtyping methods including pulsed-field gel electrophoresis, multilocus sequence typing, and WGS (including WGS-based serovar prediction) and (ii) a scoring system to evaluate and compare Salmonella subtyping assays. This literature-based assessment supports the superior discriminatory power of WGS for source tracking and root cause elimination in food safety incident; however, circumstances in which use of other subtyping methods may be warranted were also identified. This review provides practical guidance for the food industry and presents a starting point for further comparative evaluation of Salmonella characterization and subtyping methods.

show abstract

Section: Banding Pattern-based and Sequencing-based Characterization mentioning

confidence: 99%

Section: Banding Pattern-based and Sequencing-based Characterization mentioning

confidence: 99%

Assessment and Comparison of Molecular Subtyping and Characterization Methods for Salmonella

et al. 2019

View full text Add to dashboard Cite

show abstract

“…From each sample, one isolate representative of each a serogroup-antimicrobial susceptibility testing result pattern was investigated by Multi-Locus Sequence Typing (MLST). The MLST scheme followed is based on seven loci aro C, dna N, hem D, his D, pur E, suc A and thr A (Yun et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial residues, non-typhoidal Salmonella, Vibrio spp. and associated microbiological hazards in retail shrimps purchased in Ho Chi Minh city (Vietnam)

et al. 2020

View full text Add to dashboard Cite

We investigated antimicrobial residues, non-typhoidal Salmonella (NTS), Vibrio spp. and their associated antimicrobial resistance (AMR), in shrimps locally purchased in Ho Chi Minh City (Vietnam). In addition, we investigated the relationship between AMR in NTS, Vibrio spp. and antimicrobial residue in the same sample. A total of 40 samples of shrimp heads/shells from different retail sources was cultured using ISO 6579–1:2017 (NTS) and ISO/TS 21872–1:2007 (Vibrio spp.). Phenotypic antimicrobial susceptibility was investigated using Vitek (NTS, 34 antimicrobials) and disk diffusion (Vibrio spp., 12 antimicrobials). A total of 9 (22.5%) samples contained antimicrobial residue, including tetracyclines, fluoroquinolones, sulfonamides and macrolides (in 7.5%, 7.5%, 2.5% and 2.5% of samples, respectively). Shrimp samples from supermarkets had a higher prevalence of antimicrobial residue than those purchased in street markets (50% vs. 13.3%) (p = 0.049). A total of 30 (75%) samples were contaminated with NTS. All samples contained Vibrio spp., with V. parahaemolyticus being most common (87.5% samples). A total of 58.9% NTS isolates were multidrug resistant. With regards to the highest priority, critically important antimicrobials, the highest resistance corresponded to quinolones (14.4–47.8%), followed by 3rd and 4th generation cephalosporins (3.3–7.8%). Vibrio spp. isolates were characterised by their high resistance against ampicillin (82.7%) and 3rd generation cephalosporins (8.3–16.5%). Extended Spectrum Beta-Lactamase (ESBL) activity was detected in 28.1% V. parahaemolyticus isolates. Half of ESBL-positive V. parahaemolyticus strains harboured blaCTX-M1. We found an association between the presence of residues and the number of resistances for NTS (p = 0.075) and Vibrio spp. isolates (p = 0.093) from the same sample. These findings suggest that the presence of residues may contribute to the selection of AMR in foodborne pathogens in shrimps. Authorities should strengthen policies aiming at restricting inappropriate antimicrobial usage in shrimp farming, and step up monitoring of antimicrobial residues and food-borne pathogens at retail in Vietnam.

show abstract

“…The ST of five STMC strains was determined using multilocus sequence typing (MLST). The internal fragments of seven housekeeping genes ( aroC , dnaN , hemD , hisD , purE , sucA , and thrA ) were amplified using specific primers identified on the MLST website followed by DNA sequencing (Table S4) 30,31 . The sequence was compared with existing alleles in the PubMLST Salmonella genome database (https://pubmlst.org/salmonella/) to determine allele numbers and ST.…”

Section: Methodsmentioning

confidence: 99%

Pathogenicity of clinical Salmonella enterica serovar Typhimurium isolates from Thailand in a mouse colitis model

Sarichai

Buddhasiri

Walters

et al. 2020

Microbiology and Immunology

View full text Add to dashboard Cite

Salmonella enterica serovar Typhimurium (S. Typhimurium [STM]) is a leading cause of nontyphoidal salmonellosis (NTS) worldwide. The pathogenesis of NTS has been studied extensively using a streptomycinpretreated mouse colitis model with the limited numbers of laboratory STM strains. However, the pathogenicity of the clinically isolated STM (STMC) strains endemic in Thailand in mice has not been explored. The aim of this study was to compare the pathogenicity of STMC strains collected from Northern Thailand with the laboratory STM (IR715) in mice. Five STMC isolates were obtained from the stool cultures of patients with acute NTS admitted to Maharaj Nakorn Chiang Mai Hospital in 2016 and 2017. Detection of virulence genes and sequence type (ST) of the strains was performed. Female C57BL/6 mice were pretreated with streptomycin sulfate 1 day prior to oral infection with STM. On Day 4 postinfection, mice were euthanized, and tissues were collected to analyze the bacterial numbers, tissue inflammation, and cecal histopathological score. We found that all five STMC strains are ST34 and conferred the same or

show abstract

Modified Method of Multilocus Sequence Typing (MLST) for Serotyping inSalmonellaSpecies

Cited by 7 publications

References 8 publications

Assessment and Comparison of Molecular Subtyping and Characterization Methods for Salmonella

Assessment and Comparison of Molecular Subtyping and Characterization Methods for Salmonella

Antimicrobial residues, non-typhoidal Salmonella, Vibrio spp. and associated microbiological hazards in retail shrimps purchased in Ho Chi Minh city (Vietnam)

Pathogenicity of clinical Salmonella enterica serovar Typhimurium isolates from Thailand in a mouse colitis model

Contact Info

Product

Resources

About