Modified nucleoside triphosphates exist in mammals

Jiang, Han-Peng; Xiong, Jun; Liu, Fei-Long; Ma, Cheng-Jie; Tang, Xing-Lin; Yuan, Bi-Feng

doi:10.1039/c7sc05472f

Cited by 45 publications

(33 citation statements)

References 47 publications

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“…Whereas insufficient capacity for de novo dNTP synthesis triggers checkpoint activation and thus precocious cell cycle remodeling [5]. With the help of new assays, a higher sensitivity of dNTP measurement is reached [111,112]. These advances may help us in studying dynamic dNTP functions in development with higher spatiotemporal resolution.…”

Section: Resultsmentioning

confidence: 99%

The role of dNTP metabolites in control of the embryonic cell cycle

Liu

Großhans

2019

Cell Cycle

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Deoxyribonucleotide metabolites (dNTPs) are the substrates for DNA synthesis. It has been proposed that their availability influences the progression of the cell cycle during development and pathological situations such as tumor growth. The mechanism has remained unclear for the link between cell cycle and dNTP levels beyond their role as substrates. Here, we review recent studies concerned with the dynamics of dNTP levels in early embryos and the role of DNA replication checkpoint as a sensor of dNTP levels.

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Section: Resultsmentioning

confidence: 99%

The role of dNTP metabolites in control of the embryonic cell cycle

Liu

Großhans

2019

Cell Cycle

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“…While current workflows can detect the relatively low abundant dNs and dNTs in plants [2,11], reports about the detection of damaged or modified NTs which are probably even less abundant are still scarce [119,120]. Since such rare NTs are more frequently observed in non-plant systems [61,121], it is tempting to speculate that it is the complexity of the plant matrix which hampers their detection in plants. It will be necessary to boost the sensitivity to also detect these rare NTs, which may be achieved in part by further reducing the complexity of the matrix, for example, by eliminating the more abundant rNTs [11,41].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Nucleosides and Nucleotides in Plants: An Update on Sample Preparation and LC–MS Techniques

Straube

Witte

Herde

2021

Cells

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Nucleotides fulfill many essential functions in plants. Compared to non-plant systems, these hydrophilic metabolites have not been adequately investigated in plants, especially the less abundant nucleotide species such as deoxyribonucleotides and modified or damaged nucleotides. Until recently, this was mainly due to a lack of adequate methods for in-depth analysis of nucleotides and nucleosides in plants. In this review, we focus on the current state-of-the-art of nucleotide analysis in plants with liquid chromatography coupled to mass spectrometry and describe recent major advances. Tissue disruption, quenching, liquid–liquid and solid-phase extraction, chromatographic strategies, and peculiarities of nucleotides and nucleosides in mass spectrometry are covered. We describe how the different steps of the analytical workflow influence each other, highlight the specific challenges of nucleotide analysis, and outline promising future developments. The metabolite matrix of plants is particularly complex. Therefore, it is likely that nucleotide analysis methods that work for plants can be applied to other organisms as well. Although this review focuses on plants, we also discuss advances in nucleotide analysis from non-plant systems to provide an overview of the analytical techniques available for this challenging class of metabolites.

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“…In this respect, chemical labeling in combination with MS analysis has been proposed and proved to be an attractive strategy to increase the detection sensitivities of analytes ( Figure 5) [168,169]. Along this line, a variety of chemical labeling-MS-based methods have been developed for the sensitive and accurate analysis of nucleic acid modifications with the detection sensitivities of nucleosides being down to subfemtomole or even attomole levels [170][171][172][173][174][175].…”

Section: Mass Spectrometry-based Approachesmentioning

confidence: 99%

Nucleic Acids Analysis

et al. 2020

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In recent years, multi-modal entity linking (MEL) has garnered increasing attention in the research community due to its significance in numerous multi-modal applications. Video, as a popular means of information transmission, has become prevalent in people's daily lives. However, most existing MEL methods primarily focus on linking textual and visual mentions or offline videos's mentions to entities in multi-modal knowledge bases, with limited efforts devoted to linking mentions within online video content. In this paper, we propose a task called Online Video Entity Linking (OVEL), aiming to establish connections between mentions in online videos and a knowledge base with high accuracy and timeliness. To facilitate the research works of OVEL, we specifically concentrate on live delivery scenarios and construct a live delivery entity linking dataset called LIVE. Besides, we propose an evaluation metric that considers timelessness, robustness, and accuracy. Furthermore, to effectively handle OVEL task, we leverage a memory block managed by a Large Language Model and retrieve entity candidates from the knowledge base to augment LLM performance on memory management. The experimental results prove the effectiveness and efficiency of our method.

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Modified nucleoside triphosphates exist in mammals

Abstract: By establishing a chemical labeling method in combination with liquid chromatography-mass spectrometry analysis, we reported the widespread existence of various modified nucleoside triphosphates in eukaryotes.

Cited by 45 publications

References 47 publications

The role of dNTP metabolites in control of the embryonic cell cycle

The role of dNTP metabolites in control of the embryonic cell cycle

Analysis of Nucleosides and Nucleotides in Plants: An Update on Sample Preparation and LC–MS Techniques

Nucleic Acids Analysis

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