Modified pulse polarographic determination of nicarbazin in chicken tissue at the 0.1-ppm level

Wood, James S.; Downing, George V.

doi:10.1021/jf60228a041

Cited by 9 publications

(4 citation statements)

References 4 publications

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“…Most papers describing a method for the detection of nicarbazin in different matrices, use LC with UV detection [50][51][52][53][54][55][56][57][58][59][60][61][62][63][64]. Also papers describing LC-MS [4,5,59,[65][66][67][68][69], electrochemical methods [70,71] and pulse polarographic methods [70,72,73] are available.…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatographic tandem mass spectrometric determination of five coccidiostats in poultry eggs and feed

Mortier

Daeseleire

Peteghem

2005

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Liquid chromatographic tandem mass spectrometric determination of five coccidiostats in poultry eggs and feed

Mortier

Daeseleire

Peteghem

2005

Journal of Chromatography B

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“…A number of analytical techniques have been used to quantify nicarbazin. Pulse polarographic determinations were used to quantify nicarbazin in chicken tissues ( , ). Liquid chromatographic methods have been used to quantify nicarbazin in feeds ( , ) and chicken tissues ( − ).…”

Section: Introductionmentioning

confidence: 99%

Production, Characterization, and Cross-Reactivity Studies of Monoclonal Antibodies against the Coccidiostat Nicarbazin

Beier

Ripley

Young

et al. 2001

J. Agric. Food Chem.

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A cELISA was developed for the coccidiostat nicarbazin. On the basis of previous computer-assisted molecular modeling studies, p-nitrosuccinanilic acid (PNA-S) was selected as a hapten to produce antibodies to 4,4'-dinitrocarbanilide (DNC), the active component of the coccidiostat nicarbazin. Synthesis is described for the hapten [p-nitro-cis-1,2-cyclohexanedicarboxanilic acid (PNA-C)] used in a BSA conjugate as a plate coating antigen. Monoclonal antibodies (Mabs) were isolated that compete with nicarbazin, having IgM(kappa) isotype. Because of the lack of water solubility of nicarbazin, N,N-dimethylformamide (DMF) (3%, v/v) and acetonitrile (ACN) (10%, v/v) were added to the assay buffer to achieve solubility of nicarbazin and related compounds. The Nic 6 Mabs had an IC(35) value for nicarbazin of 0.92 nmol/mL, with a limit of detection of 0.33 nmol/mL. Nic 6 exhibited high cross-reactivity for PNA-S and PNA-C, and 3-nitrophenol, 4-nitrophenol, and 1-(4-chlorophenyl)-3-(4-nitrophenyl) urea. However, Nic 6 had little or no cross-reactivity with 15 other related compounds.

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“…Other methods include voltametric analysis by the Osteryoung square-wave technique 18 and differential pulse polarography. 19,20 The AOAC method 21 which is only specific for feeds uses a 30 g alumina column (500 3 22 mm id) with dimethylformamide (DMF) and alcohol as solvents for extraction followed by a clean-up procedure and finally spectrophotometric determination at 430 nm. The method is not ideal as it takes a long time (6 h), using large volumes of a potentially harmful solvent (DMF) and can only detect niacarbazin in feed (not eggs or muscle tissue) at levels of 25-125 ppm.…”

Section: Introductionmentioning

confidence: 99%

A new and rapid method for the determination of nicarbazin residues in poultry feed, eggs and muscle tissue using supercritical fluid extraction and high performance liquid chromatography

Matabudul¹,

Crosby²,

Sumar³

1999

Analyst

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A simple and rapid method, using supercritical fluid extraction (SFE) with off-line high-performance liquid chromatography for the isolation and determination of nicarbazin, a popular broad-spectrum coccidiostatic drug used principally in poultry, is described. Results show good repeatability with a minimum quantification level of 0.4 microgram g-1 and mean 'spiked' recoveries of 98%, 100% and 99% using poultry feeds (n = 18), eggs (n = 28) and chicken tissue (n = 20), respectively. SFE using carbon dioxide is proposed as an alternative isolation method to the current Association of Official Analytical Chemists (AOAC) procedure which involves the use of large volumes of a harmful solvent (dimethylformamide) and requires a long tedious separation and clean-up regime (6 h) prior to its determination.

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Modified pulse polarographic determination of nicarbazin in chicken tissue at the 0.1-ppm level

Cited by 9 publications

References 4 publications

Liquid chromatographic tandem mass spectrometric determination of five coccidiostats in poultry eggs and feed

Liquid chromatographic tandem mass spectrometric determination of five coccidiostats in poultry eggs and feed

Production, Characterization, and Cross-Reactivity Studies of Monoclonal Antibodies against the Coccidiostat Nicarbazin

A new and rapid method for the determination of nicarbazin residues in poultry feed, eggs and muscle tissue using supercritical fluid extraction and high performance liquid chromatography

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