Modified salting-out method for DNA isolation from newborn cord blood nucleated cells

Abstract

The present work describes modification of a widely used salting‐out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the β‐globin gene. The salting‐out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re‐extract the samples. J. Clin. Lab. Anal. 14:280–283, 2000. © 2000 Wiley‐Liss, Inc.

Pyrimido[1,2-a]-purin-10(3H)-one, M1G, is less prone to artifact than base oxidation

“…In this method, the hydrolyzed proteins were precipitated from the sample by salting out as described previously ( 25 , 26 ) with minor modifications. A 2 ml aliquot of 5 M NaCl was added to the tissue lysate followed by vigorous shaking for 1 min.…”

Association Between Cytochrome 1B1*3 Polymorphism and the Breast Cancer in a Group of Iranian Women

“…DNA was extracted from the blood samples using the saturated salt method (22) and the extracted DNA concentration was examined in all the samples. Specific primers complementary to the target area and previously used were selected to perform the polymerase chain reaction (23) in the following order:…”

Lack of association between ACE ID genetic polymorphism and diabetes or hypertension in Brazilians aged from 50 to 70 years old

“…10 The ACE I/D polymorphisms (rs 1799752) were amplified by the polymerase chain reaction (PCR) and the products from the amplification were genotyped by electrophoresis in agarose gel. The primers used were F-5'-CTGGAGACCACTCCCATCCTTTCT-3' and R-5'-GATCTGGCCATCACATTCGTCAGAT-3'.…”