Modifying Effect of Diallyl Sulfide on Colon Carcinogenesis in C57BL/6J-Apc^Min/+Mice

Abstract

Diallyl sulfide (DAS), a flavoring compound derived from garlic, is considered to have cancer chemopreventive potential in experimental animals and humans. This study was designated to examine possible chemopreventive effects of DAS on colon carcinogenesis using genetically engineered transgenic Apc Min/+ mice, a well-established animal model for familial adenomatous polyposis (FAP) and sporadic colorectal cancer. Male C57BL/6J-Apc Min/+ mice were divided into three groups. Animals of group 1 were placed on the basal diet (AIN-76A) as non-treated controls. Animals of groups 2 and 3 were given DAScontaining diets (in doses of 100 and 300 ppm, respectively). All mice were sacrificed at the end of week 10 of the experiment. Histopathological investigation revealed that the incidence of colonic polyps was decreased dose-dependently by 19% (13/16) in group 2 and by 32% (13/20) in group 3 compared to the 100% incidence (10/10) in group 1. The multiplicity of colonic polyps per mouse was also slightly decreased by DAS treatment (1.88±0.35 in group 2 and 1.63±0.36 in group 3) compared to 2.00±0.39 in group 1. On the other hand, there were no significant differences in the numbers of total polyps per mouse in the small intestine between the groups. Taken together, we suggest that DAS may exert promising inhibitory effects on colon carcinogenesis in the transgenic Apc Min/+ mice.

Chronic diseases, inflammation, and spices: how are they linked?

“…DAS has also been demonstrated to have anticancer properties against different cancers such as colon cancer, prostate cancer, skin cancer, etc. via modulation of inflammatory pathways [ 82 – 84 ].…”

Diallyl Sulfide: Potential Use in Novel Therapeutic Interventions in Alcohol, Drugs, and Disease Mediated Cellular Toxicity by Targeting Cytochrome P450 2E1

“…DAS treatment demonstrated a dose dependent inhibition towards expression of MMP-2 and −7 in colo 205 cells indicating its potential anti-metastatic properties. In animal model for familial adenomatous polyposis and sporadic colorectal cancer, DAS significantly reduced the incidence of colonic polyps in a dose-dependent manner [28]. Furthermore, results from a meta-analysis demonstrated a clinical association between high garlic intake and decreased risk for stomach and colorectal cancers [29].…”

Organosulfur Compounds in Colorectal Cancer Prevention and Progression

“…A separate study testing the effects of dietary DAS supplementation in APC min mice found that supplementation with 100 to 300 ppm reduced the incidence of colonic polyps in a dose-dependent manner, although the inhibition was not statistically significant [ 109 ]. The authors claim that the lack of statistical significance is likely due to the relatively small number of mice used.…”